| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,368,101 | T→C | 100% | A21A (GCT→GCC) | arnA → | fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,368,101 | 0 | T | C | 97.1% | 38.2 / ‑6.4 | 18 | A21A (GCT→GCC) | arnA | fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (6/11); total (6/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCG > NC_000913/2367977‑2368233 | cgcgCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGCTATGGGATGCCTCGGTCTTGCAGCCCCGCTGGCCGCCGGTTACGaaagtcgc > 1:38975/1‑138 (MQ=255) ccttcTAGTTTTTTCGTCAAGTTTTCCGTCCATCCATCAAGGAAAATGAATAATGAAATCCGTCGTTTTTGCCTACCACGATATGGGATGCCTCTGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTa < 2:37515/139‑1 (MQ=255) ttctGTTCAGCAAGTTATCCGTCCATCCAGCAAGGATAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATAc < 2:126253/140‑1 (MQ=255) tgtgCAGCAGTTTATCCGTCTATTCAGCAAGGACACTGAATAATGATTACCGTCGTGTTTGCCTACCCCGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGa < 2:207818/143‑1 (MQ=255) gCAAGTTATCCCTCCATCCAGCAAGCAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGTTATGGGATGTCTCTGTATTGAATCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATcc < 1:382917/143‑1 (MQ=255) aGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGATTACGAAATTCGCGCCATTTTTACCCATACTGCTAATCCCGGTGAAAAAGCCTTTTAt > 2:142297/1‑143 (MQ=255) aacaTGAATAAGGAAAACCGGCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCAGTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCgg < 2:429625/140‑1 (MQ=255) aacttaatactGATAACCGTCGTTTTTGCGTCCCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGt < 2:339262/138‑1 (MQ=255) aaaTGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGATGCCCTGTTGGTCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGt < 1:500894/143‑1 (MQ=255) tctaatGGTAACCGTCGTTTTTGCCTACCACGATCGGGGATGCCTCGGTATTGAAGCTCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTTCCCATACTGATACTCCCGGTGAAAAAGTCTTTTATGGTTCGGTGGCTc < 2:212474/141‑1 (MQ=255) tttGCCCACCCCTATTTGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACTAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAgg < 1:362076/143‑1 (MQ=255) cTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGACAGAGGCGTTc > 1:143864/1‑143 (MQ=255) tACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAACAAGCCTTCTATGGTTCGGTGGCTCGTCTGACAGCGGAAACACGCATTcc > 1:241795/1‑143 (MQ=255) catatGGGAGGTCGCGGTATTGAACACGCGCTGTCTGCGGGTTAAGAGATTAGCTCGATTTTTACGCATATTGTTGATACTTGTGAAAAAGGTTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTa < 2:63609/142‑1 (MQ=255) cTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGTACTTAGCGCCATTTTTTCCCATACTGATGGTCCCGGTGAATAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATaa < 2:38975/143‑1 (MQ=255) aaGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAatcat > 1:516085/1‑143 (MQ=255) aGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGCTCGCTCCCTCCCCCGCCGGCGGAAAGAGGCATTTCGGTTTATGCGCCGGATAACGTTAatcatc > 1:419666/1‑143 (MQ=255) cccTGCTGGCCGCCGGTTACGAAATTAGCGCCTTTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCg < 1:535677/143‑1 (MQ=255) | CGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCG > NC_000913/2367977‑2368233 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |