| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,661,821 | C→G | 100% | C104S (TGC→TCC) | iscR ← | DNA‑binding transcriptional dual regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,661,821 | 0 | C | G | 99.5% | 67.9 / ‑7.1 | 26 | C104S (TGC→TCC) | iscR | DNA‑binding transcriptional dual regulator IscR |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (12/13); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCA > NC_000913/2661694‑2661955 | gtcgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCt > 1:307258/1‑143 (MQ=255) gCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTCCGCCTAAAGTAACGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGCCAGGGCTCTATCGCCGCCCCGgccgccgccc > 1:226789/1‑142 (MQ=255) tCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGt < 1:325995/143‑1 (MQ=255) ccAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTg > 1:151436/1‑143 (MQ=255) ccAGCACTACCTGGTTATTATCCAGTTCGCCTAAAGGAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTg < 1:660/143‑1 (MQ=255) cAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTCCCCTGACAACGGGTgc > 2:20684/1‑142 (MQ=255) ttCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTa > 1:61558/1‑143 (MQ=255) cTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCCCCCTGGCCGCCGCCTTTGCCCTGCCAACGTGAGGCACCTCCAg > 2:393220/1‑143 (MQ=255) taACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCCGCGCTTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGACATCTACAGATTCGTCa > 1:42025/1‑143 (MQ=255) aaCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTCGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATCCGTCaa > 2:367424/1‑143 (MQ=255) cggTTCTCCGAAAGTAATGTTGTTGAGAGAGCCGGTGAGACGGTCGTTCAAATTACGCCCCAGCGCGTGGGCCAGGGATTTATCGCCGCCCCGGCAGCAGCTTTTGCCCTGACAACGGGTGGCATCTACAGATTCGTCAACgg < 2:164478/141‑1 (MQ=255) ttCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGgcgc > 2:422179/1‑143 (MQ=255) gagaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTACTTACTTCGCCAACGGCGa > 1:146497/1‑143 (MQ=255) aaaCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATAACCGCCCTGGCAGCCGCCTTTACCCTGACCACGAGCGGCATCTACAGATTCGTCAACGGCGCGAATTGCTTCCCCAAAAGCGAtagg > 2:176860/1‑140 (MQ=255) cGGTCGCGCACATCACGCCGCGGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCttt < 2:265974/143‑1 (MQ=255) ggcgggcAAATGACGCCAGAGCGCGTGGGGCAGGGATGTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGc < 1:180563/137‑1 (MQ=255) ctctcgtaaaTCACGCCGCGGCGCTGGGGTCAGGGTTTTATCGCCGCCCTGGTAGCCGCCGTTACCGTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGc < 2:54861/136‑1 (MQ=255) cTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTaa < 1:510362/143‑1 (MQ=255) aaTCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGa < 1:339226/143‑1 (MQ=255) aaTCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGa < 1:74938/143‑1 (MQ=255) tCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATa < 2:151436/143‑1 (MQ=255) cgcGCCCCAGCGCGTGGGTCAGGCATTTCTCGCCGCCCTGGCAGCCGCCTGTACCCTGACAACGGGTGGAATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATaa < 2:425989/141‑1 (MQ=255) cACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATaa < 1:354651/143‑1 (MQ=255) gTGGGTCGGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCAGCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAGGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTc < 2:39269/143‑1 (MQ=255) gTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTc < 1:168565/143‑1 (MQ=255) gggTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCa > 1:400767/1‑143 (MQ=255) | GTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCA > NC_000913/2661694‑2661955 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |