| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,634 | A→C | 100% | T108P (ACC→CCC) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,634 | 0 | A | C | 100.0% | 121.1 / ‑6.7 | 41 | T108P (ACC→CCC) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); major base C (16/22); minor base T (0/1); total (17/24) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTAT > NC_000913/3041500‑3041769 | gCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATcgcg < 1:331520/143‑1 (MQ=255) cGACGCGAAAGGTAAAATGTGGAGCAATGTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCgcgc < 1:473938/143‑1 (MQ=255) gACGCCACAGGTACAACGCAGAGCAAAATACGTCTGCCCCACGACACACATTGCTTAGCAAAGATTGAAGAGAAGAGCATGCATGAACCGCAACCCACGAAACCGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGcc < 2:253822/143‑1 (MQ=255) gCCAAAGGTAAAATGTAGAGCAATTTACGTCTGTTACGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGCGAACCGCAGCTGACAGAACTGAAAAAATATGCGGTGATATCTAAAGTGGCAATCGGGccgca > 2:207388/1‑140 (MQ=255) aaaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAgacga < 2:139436/143‑1 (MQ=255) tAAAATGGGGAGCAATTTAGGTCTGTTCCGCGACGGCGATGGCGTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTACAGTGTCCATCGCGCCAGACGACGAGc < 2:170211/143‑1 (MQ=255) aaaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGCCGACGAGCgt > 1:539882/1‑143 (MQ=255) aaaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTAGTCTCCACAGTGCCCATCGCGCGAGACCACGACCgc > 2:625/1‑142 (MQ=255) gCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCtt > 2:73102/1‑143 (MQ=255) gCAATTTACGTCTGTTCCGCGACGGCGATGGCTCTGCATGGACTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCtt < 1:87232/143‑1 (MQ=255) cAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGCCTGAACTGAAAAAATATGCGGTATTCTCTAAACTGCCCATCGCGCCAGCCGACGAGCGTGCGCTCCTTg > 1:407246/1‑143 (MQ=255) aaTTTAGGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTgg < 1:285458/143‑1 (MQ=255) atgtACGGCTGTGCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgt < 2:435297/140‑1 (MQ=255) tttACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtg > 1:407548/1‑143 (MQ=255) gtgaCGTCTGTTCCGGGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtg < 2:317039/140‑1 (MQ=255) cGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGc < 2:454025/143‑1 (MQ=255) tccgtcccGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAAGATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg < 1:196069/137‑1 (MQ=255) tGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGt > 2:328203/1‑143 (MQ=255) gcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAg < 2:128441/143‑1 (MQ=255) gACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgc > 1:497123/1‑143 (MQ=255) ggCTTTGAATGGATTGATCGGCGCTGCGTGCGTGAACCGGTGCTGGCTGTGGTGGGCTTGTGTGCGGTTTTCTCTAAAGTGCCCATCGCGCCAGACGTCGTGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgc < 2:10160/143‑1 (MQ=255) aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaa > 1:254722/1‑143 (MQ=255) ggATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAt < 1:377529/143‑1 (MQ=255) tGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct < 1:186580/143‑1 (MQ=255) cgaccGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct < 2:367860/139‑1 (MQ=255) ggaCGGCGCAGCGTGCGTGAACCGCAGCTGACAGAAAAGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCtt < 2:165007/141‑1 (MQ=255) cgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCtcc > 2:125946/1‑141 (MQ=255) cgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTc > 2:396511/1‑143 (MQ=255) gtaCCGCAGCTGACTGAGCGCAGCACATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaa < 2:462526/141‑1 (MQ=255) gCAGCTGACTGAACTGAAAAAATATGCGGTATTCTATAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGGTTTCAGGCGCGCGCCGCGCTGGCAAAACTCTTTAGCGCACTGCCTGCgcaagaaa > 2:476381/1‑143 (MQ=255) agatgactaaAGAGAACAAAGATGCGGTAGTCTCTAAAGTGCCCGTCGCGCCAGAGGGCGAGCGTGTGCTGCTTGGTGTTGAGGGTTTTCAGGCACGGGGCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGaaaaa < 2:89423/140‑1 (MQ=255) aGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGaaaaa < 1:497678/143‑1 (MQ=255) gCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAAc > 1:468316/1‑143 (MQ=255) tgaaAAAATATGCAGTATTATCTAAAGTGACCATCGAGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGACGGTTTTCAGGCGCGCGACACGCTGGAAAATATCATTAGCGAACTACCTTAAAAAGAAAAACAGGTAGTCaaa < 2:147892/143‑1 (MQ=255) aaaaaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaa < 1:328203/143‑1 (MQ=255) aaaaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaag > 1:429015/1‑143 (MQ=255) tGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTGGTCAAAGAAGGCGCGa > 2:438084/1‑143 (MQ=255) gCGGTAGTCTCTAGAGTGCCCATCGCGGCAGACGACGAGCGGGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGAc < 2:252005/143‑1 (MQ=255) cGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGATTTCCGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGCAAGCAAAACAGGTAGTCAAAGAAGGCGCGAcc > 1:524243/1‑143 (MQ=255) tctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCATTAGCGAACTGCCTTCGAAAGGAATAGAGGGAGTCAAAGACCGCGCGACTACTTTgat > 1:273136/1‑141 (MQ=255) tAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTAt < 1:412774/143‑1 (MQ=255) | GCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTAT > NC_000913/3041500‑3041769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |