| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,441,060 | T→C | 14.9% | K206E (AAG→GAG) | rpsD ← | 30S ribosomal subunit protein S4 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,441,060 | 0 | T | C | 14.9% | 54.5 / 4.5 | 27 | K206E (AAG→GAG) | rpsD | 30S ribosomal subunit protein S4 |
| Reads supporting (aligned to +/- strand): ref base T (11/12); new base C (2/2); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-02 | |||||||||||
ACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGA > NC_000913/3440931‑3441202 | aCGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTCCAAGTAAAGCtta < 2:32007/143‑3 (MQ=255) aGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAgg > 2:371575/1‑143 (MQ=255) gtcgtaaGCGTCACCTTGGCGTGCGTCGAACTCACTTGTTCGATATCAACCAGGCGCGGTTTTAGAAACGCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGgt < 2:340804/138‑1 (MQ=255) ttGGGGTGCGTCGAACTCGCTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCg < 2:151063/143‑1 (MQ=255) gcgtgcgtCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGcag < 1:51442/143‑1 (MQ=255) aCTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGTTCAGGTCTTCTTTAATGTCCGGAGACAGATCAGaa > 2:153251/1‑143 (MQ=255) aCTCACTTGCGCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGaa < 2:450235/143‑1 (MQ=255) tCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACg < 1:168720/143‑1 (MQ=255) aCTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATGGTGTCCTCTCTTTGGTACTAATCTTTACTTGGCGCCCAGTTCGAAGAGCAGCTGCTCGTTAATGTACGCCGACAGCACAGAACGCt > 2:213309/1‑143 (MQ=255) ttGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTcc > 1:376023/1‑143 (MQ=255) tCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCGGGCt > 1:218448/1‑143 (MQ=255) tCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTCCAAGTAAAGCtt > 1:522695/1‑92 (MQ=255) tCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTCCAAGTAAAGCtt < 2:522695/93‑2 (MQ=255) ggTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTCAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCtt > 2:285326/1‑143 (MQ=255) aaCTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGc < 1:27049/143‑1 (MQ=255) ctctGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCa < 2:376023/143‑1 (MQ=255) gTCACAGCACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCGCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCAt < 2:196527/143‑1 (MQ=255) gcTGCATTGTGTCGGCTCTTTGGTAGTAGGCTTTACTTGTGGTAGAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCGCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTccag < 2:235298/142‑1 (MQ=255) ccTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCTAGCATCAACCTccag > 2:122279/1‑143 (MQ=255) ccTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTccag > 1:490941/1‑143 (MQ=255) cTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTccagc > 1:497060/1‑143 (MQ=255) tGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTccagcc > 1:498466/1‑143 (MQ=255) tGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCACCTTGCCAGCACCAACTTccagcc > 2:290016/1‑143 (MQ=255) tCCTCTCTTTGGTGCTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGGTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCt < 2:107077/143‑1 (MQ=255) tttGGTACTAAGCTTTACGTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACg < 1:371575/143‑1 (MQ=255) aaGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTGCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTcagc < 2:349016/143‑1 (MQ=255) aGCTTTACTCCAAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGCCTCTGCACGCTGCTcagcc > 2:270693/1‑143 (MQ=255) tGGAGTAAAGCTCGACGATCAAGAGTTCGGTAATGTCCGCAGACAGATCAGAACGCTCCGACTTACGATTAAACGTACCTTCCCTCTTCCCACCATCAACTTCCCGCCAGGCTGCGTTTCTACACTCCACAGCAAGCTccccg > 2:69437/1‑140 (MQ=255) | ACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGA > NC_000913/3440931‑3441202 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |