| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,733,502 | G→A | 12.7% | R434R (AGG→AGA) | xylG → | xylose ABC transporter ATP binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,733,502 | 0 | G | A | 12.7% | 62.4 / 3.7 | 35 | R434R (AGG→AGA) | xylG | xylose ABC transporter ATP binding subunit |
| Reads supporting (aligned to +/- strand): ref base G (15/13); new base A (2/2); total (19/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.76e-01 | |||||||||||
ATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCCAGCAGGGTATTGCCG‑TTATTGTCATCTCTTCCGAATTACCTGAAGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAG > NC_000913/3733361‑3733641 | atcactcCAGCAACTCACAGTTGAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCATCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAggg < 2:452466/143‑1 (MQ=255) cAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCGATCACAAACACATCG‑TCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATCCTTGATGAACCCACCAGGGGTATCGa > 2:190319/1‑143 (MQ=255) aCTCAAAGTTAAACCGGCGTCGCCCCACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAA‑AAAGCGATCCGCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTGATTCTTGATGAACCCACCAGGGGTATCGATATTg < 2:478192/143‑1 (MQ=255) aCTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTg < 1:46199/143‑1 (MQ=255) tCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTCACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTGTCGATATTGgc > 1:209879/1‑143 (MQ=255) cAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGgcg > 2:317557/1‑143 (MQ=255) aGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTACCGATATTGCCGCGa > 2:349406/1‑143 (MQ=255) ggtAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGaa < 1:490525/141‑1 (MQ=255) ttAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGaaa > 1:454828/1‑143 (MQ=255) tcCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTa < 2:204302/143‑1 (MQ=255) tttGCTGTTGGGCGGTTGAGGGGCGGCAATCAGCAC‑AAAGCGATGCGCGCTCGCTGTCTGTGACTTAAGCCGCGCATTCTCATTCTTGATGAACCCACGAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAaattaat < 1:511291/142‑1 (MQ=255) gCTATTGGCCGTTTGCGCGGCCGCAATCAGCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGTGTATCGATATTGGCGCGAAATACGAGATCTACAAAttaattaa < 2:483834/143‑1 (MQ=255) ctgtttgACGTTTGGCCGGCTGCCATCAGCAA‑CAAGCGTTCATCGCCCGCTGTCCGTTACTTAACCCGCGCATTCTCATTCCTGTGGAACCCACCATGTGTATCGATATTGGCGCGAACTACGAGATCTACAAATTAATTAAc < 2:421429/137‑1 (MQ=255) cTATTGGACGTTTGAGCGGCGGCAATCAGCAA‑AAAGCGATCCGCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGCGGTATCGATATTGGCGCGAAATACGAGATCTAACaattaagtcac > 2:417805/1‑138 (MQ=255) tATTGGACGTTTGAGCGGCGACAATCAGCAA‑AAAGCGATCCTCACTCGCTGTCTGTTACTTAACCAGCACATTCTCATTCTTGATAAACACACAAGTAGTATCGATATTAACACGAAACACCAGATCAACAAAaaaattaaaa > 2:516097/1‑141 (MQ=255) ttGGACGTTTGAGCGGCGGCAATCAGCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGAGCAACCCACCAGGGGGATTGATATTGGCGCGCGATACGAGAGCCAAAaattaatgtaccca > 2:264666/1‑136 (MQ=255) aCGTTTGAGCGGCGGCAATCAGCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGAGGTATCGATATTGGCGCGAAATACGAGATCTACGAATTAATTAACCAACTCg > 2:2592/1‑143 (MQ=255) aCGTTTGAGCGGCGGCAATCAGCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATGCTCATTCTTGATGAACCCACCAGAGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCg > 2:8236/1‑143 (MQ=255) gTTTGAGCGGCGGCAATCAGCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATCCTTGATGCACCCACCAGGGGCATCGATATCGGCGCGCAATACGAGACCCCCAAATTCATTAACCAACCCgcc > 2:13329/1‑141 (MQ=255) cAATCAGCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACGCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAGAGTAATTAACCAACTCGTGAAGCAGGGTCTTg > 2:327924/1‑143 (MQ=255) aGCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATAAATTAACCAACTCCTCCAGCAGAATAATGCCgca > 2:336201/1‑141 (MQ=255) ataccgCGTTCCACGTTCGCTTTCTCTCCCTTAACCCGCGCATCCGCATTCTTCATTGACTCACCCAGCGTATCGATATTGCCGCTAAATACGAGATCTACAAATTAACTACCCAACTCGTCCAGCAGGGTATTGCCG‑TTAtt < 2:87532/138‑1 (MQ=255) tcGATCCTCGCTCGCTGTGTGTTACATAACCCGCGCATTCTCATTCTTGATCAACCCACCAGTGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCCAGCAGGGTATTGCCG‑TTATTGTCAt < 2:31434/142‑1 (MQ=255) tgtTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCCAGCAGGGTATTGCCG‑TTATTGTCATCTCTTCCGAATTACCTGaa < 1:317557/143‑1 (MQ=255) tgtTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGAGTAATACGAGGTCTACAAATCAATTAACCAACTCGTCCCGTGGGGTA‑GGCCGCGTATTGGCATCACTTCCGACTTAcatagc > 2:520749/1‑138 (MQ=255) cTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCCAGCCGGGTATTGCGG‑TTATTGTCGTCTCTTACGGATTAACTAAAGTggg > 1:324813/1‑141 (MQ=255) cTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCCAGCAGGGTATTGACG‑GTCTTGTCATATCATGCGAATTACCCGAAGtaca > 1:485677/1‑140 (MQ=255) ttAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTCATTAACCAACCCGTCCAGCAGGGTATTGCCG‑TGATTGTCACCCCATCCGACGTACCAGAAGCGCta > 2:81697/1‑142 (MQ=255) ttAACCCGCGCATTCTCATTCTTGATGAACCCACCAGAGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCCAGCAGGGTATTGCCG‑TTATTGTCATCTCTTCCGAATTACCTGAAGTGCTc < 1:2592/143‑1 (MQ=255) ttAACCCGCGCATTCTCATTCTTGATGAACCCACCAGAGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCCAGCAGGGTATTGCCG‑TTATTGTCATCTCTTCCGAATTACCTGAAGTGCTc < 1:8236/143‑1 (MQ=255) gcATTCTCATTCTTGATGAACCCACCGGGTGTATGGTGCTGCGTGCGTAATACGAGATCTACAAATTAATTAACCAACTCGTCCAGCAGGGTATTGCCG‑TTATTGTCATCTCTTCCGAATTACCTGAAGTGCTCGGCCTTAGc < 2:521190/143‑1 (MQ=255) gcATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCACCTCGTCCAGCAGGGTATTGGCG‑TTAGTGTCATCTCTTCCGAATTCCCTCCAGTCCTCTGCCttgta > 1:523906/1‑140 (MQ=255) cattctcattctTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTAAAAAGTAATTAACCAACTCGTCCAGCAGGGTATTGCAG‑TTATAGTCATCACATCCGACGTACCTGAAGTGCTCCGctctaccg > 2:162960/1‑136 (MQ=255) ctTGACGAACCCACCAGGGGTAGCGATATTGCCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCCAGCAGTGTATTGCCG‑TTATTGTCATCTCTTCCGAATTACCTGAAGTGCTCGGCCTTAGCGATCGTGTACt < 1:378169/143‑1 (MQ=255) aCCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCCAGCAGGGTATTGCCG‑TTATTGTAATATCTACTGATGTCCTTGAAGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGc < 1:368820/143‑1 (MQ=255) accccgggggTATCGGTATGGGCGCGAAATACGAGATCTACAAATTACTTACCCAACTCGTCCAGCAGGGTATTGCCG‑TTATCGTCATCTCTTCCGAATTACCTGAAGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGCAt < 1:190319/137‑1 (MQ=255) caGGGGTATCGATATTGGCGCGAACTACGAGATCTACAAATTAATTAACCAACTCGTCCAGCAGGGTATTGCCG‑TTATTGTCATCTCTTCCGAATTACCTGAAGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAg < 1:43518/143‑1 (MQ=255) | ATCAATCCAGCAACTCAAAGTTAAAACGTCGTCCCCCGACCTTGCTATTGGACGTTTGAGCGGCGGCAATCAGCAA‑AAAGCGATCCTCGCTCGCTGTCTGTTACTTAACCCGCGCATTCTCATTCTTGATGAACCCACCAGGGGTATCGATATTGGCGCGAAATACGAGATCTACAAATTAATTAACCAACTCGTCCAGCAGGGTATTGCCG‑TTATTGTCATCTCTTCCGAATTACCTGAAGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAG > NC_000913/3733361‑3733641 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |