| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,236,011 | T→C | 18.2% | S36P (TCG→CCG) | yjbE → | uncharacterized protein YjbE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,236,011 | 0 | T | C | 18.2% | 32.6 / 2.2 | 22 | S36P (TCG→CCG) | yjbE | uncharacterized protein YjbE |
| Reads supporting (aligned to +/- strand): ref base T (11/6); new base C (2/2); total (13/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01 | |||||||||||
CTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAA > NC_000913/4235877‑4236152 | ctcCATTCATTCAATGAAGGGAGATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGCCGGTTTcg > 2:366793/1‑143 (MQ=255)ctcCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGCCGGTTTcg > 1:51126/1‑143 (MQ=255) ttcaATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGTGCCGCAGCAGTACCCGTGCGCGACGCCGCAGGGTCCGCAGCAACGTCGGTTTCGGCCGGGAGt > 2:468138/1‑143 (MQ=255) ttcaATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGt > 1:291986/1‑143 (MQ=255) aaTGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGCGGGCTGCACCTGTACAGGTGGGCGCAGCGGCCGGGTCGGCCGCAACGTCGGTTTCGGCGGGGGGCtcc > 2:171607/1‑143 (MQ=255) ggAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGTGCGAAGCGGCAGGCTCGGCAGCAACGTCGGTTTCGGCGGGCAGTTCGTCCGCGa > 1:450220/1‑143 (MQ=255) aaaaGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCCGCGTCAGCCCCg > 2:366762/1‑143 (MQ=255) gTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGATCCTCCGCGACCAGCGTCAGCACCGTGAGCGcgg > 2:466811/1‑143 (MQ=255) ggCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCggt > 1:382190/1‑143 (MQ=255) ttCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGCTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACcggcggcgggg > 2:76897/1‑141 (MQ=255) tGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATg < 2:269728/143‑1 (MQ=255) tGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAGCGTCGGTTTCGGCGGGGAGTTCCTCCGCGAACAGCGCCAGCACCGTACGCTCGCCGGTGGGCGCCGCGCTTGCGCCAACCGGAAGCGGCGAAGGTTccgaca > 2:280866/1‑138 (MQ=255) tACAGGTGGGCGAAGCGGCAGGGGCGGCAGCGACGTCGGTTTCGGCGGGGAGTTCCTGCGCGACCGGCGTCAGCACCGTAAGCTCGGCGGGGGGTGTCGCGCTTGCGGCAACCGGTGGCGGAGATGGTTCTAATACCGGGacc > 1:294553/1‑143 (MQ=255) cAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGaccac < 1:466811/143‑1 (MQ=255) cgCGGCATCGTCGGCAGCAACGGCGGTCTCGGCTGGGCGTTCCGCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGCTATCGCGCTTGCGCCTACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGa < 2:159306/142‑1 (MQ=255) aGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGa < 1:31261/143‑1 (MQ=255) aGGGTCGGCAGCAACGCCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCTGCGGTGGGTGTCGTGCTTGCGTCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGAccacca < 1:366793/143‑1 (MQ=255) aGGGTCGGCAGCAACGCCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGAccacca < 2:51126/143‑1 (MQ=255) gTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCCGTa > 1:525749/1‑143 (MQ=255) gcagcaACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCa < 1:163622/143‑1 (MQ=255) gcaCCGTCGCTTTCGGCGGGGGGTTCCTCCGCGCCCGGCGTCAGCACCGTAAGCTCGGCGGTGGGTTTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGta < 2:479840/143‑1 (MQ=255) gTCGGTTTCAGCGGGGAGTCCCCCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATaaa < 1:284596/143‑1 (MQ=255) | CTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAA > NC_000913/4235877‑4236152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |