Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,365,452 G→T 12.0% intergenic (‑96/+20) cutA ← / ← dcuA copper binding protein CutA/C4‑dicarboxylate transporter DcuA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,365,4520GT12.0% 65.2 / 4.1 34intergenic (‑96/+20)cutA/dcuAcopper binding protein CutA/C4‑dicarboxylate transporter DcuA
Reads supporting (aligned to +/- strand):  ref base G (16/14);  new base T (2/2);  total (18/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01

ACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGT  >  NC_000913/4365319‑4365588
                                                                                                                                     |                                                                                                                                        
acGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTTCACGCCCcg                                                                                                                                 <  2:519638/143‑1 (MQ=255)
acGACAGACGCGGGATTCGAACTTTTTTCATCAAGCATAAACACCGCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTTCACGCCCcg                                                                                                                                 <  1:130955/143‑1 (MQ=255)
        cgcgGTATGCGAACTTTTTTCATCAAGCAGAAACACCTCGCTAGGACAGATGGAACCGCAAGAGAGAGGGCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGtgattgat                                                                                                                         <  2:536265/143‑1 (MQ=255)
         gcgGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAGGAGAGAGGTCACTATACAACGGACGGGGGGAGGATGTTAGTAGGCGAGAAAGAAAGCGGGGCGTGGAAGCCTAGCGagcgagc                                                                                                                        >  2:98155/1‑137 (MQ=255)
                      ttttttCATCAAGCGTAAGCACCTCATCAGCACAGATGGAACCGCAAGAGCGTGGTCACTATACAACGGACGGGGGAAGGATGTGAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGaa                                                                                                           <  2:403063/143‑1 (MQ=255)
                              tcaAGCATGAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCa                                                                                                   <  2:529517/143‑1 (MQ=255)
                                  gCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGccc                                                                                               >  1:168997/1‑141 (MQ=255)
                                     tAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCAcgaa                                                                                            >  1:158407/1‑143 (MQ=255)
                                     tAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGACGTTAGTCGGCGGCAAAGAAAGCGGGGCGTGAACGCACCGAGATAGATCACTCCAGCACGACGCTACCCAacaccca                                                                                            >  2:485220/1‑140 (MQ=255)
                                      aaaCACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGACGCTACCCAGCGcgacg                                                                                           >  1:34820/1‑141 (MQ=255)
                                          acCTCGCGAGAGCGGATGGAACCGCAGGAGAGAGGGCACGATGCAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAGGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCAcgaagccga                                                                                       <  2:344367/143‑1 (MQ=255)
                                            cTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATGGATCATTACAGCATGAAGCTACCCAGCAcgaagccgaag                                                                                     >  1:330424/1‑143 (MQ=255)
                                                  ataaCGGATGGAATCGCAAGAGAGAGGTCACTATACAAGGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGCCAGCGTGTCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACg                                                                               <  2:496718/141‑1 (MQ=255)
                                                     aCAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGAGTGATCATTACAGCATGAAGCGACCCAGCACGAAGCCGAAGCAAACGGcc                                                                            >  2:519129/1‑143 (MQ=255)
                                                              aCCGCAAGGGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAaca                                                                   <  1:264235/143‑1 (MQ=255)
                                                                        agagGTCACTATACACCGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACAACCAGGGCAACACCCAGAGTAc                                                         >  2:453259/1‑143 (MQ=255)
                                                                           ggTCACTATAGAACGGACGGGGGAAGGCTGTTAGTCGGCCGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCg                                                      <  2:78491/143‑1 (MQ=255)
                                                                              catgaaaCAGCGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGa                                                   <  1:69952/137‑1 (MQ=255)
                                                                              cagtataAAACGGGGGGGGGAAGGGTGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGa                                                   <  2:168997/140‑1 (MQ=255)
                                                                              cACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGa                                                   <  1:116008/143‑1 (MQ=255)
                                                                                      gacggacggGGGAAGGATGCTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAAc                                           <  2:130886/142‑1 (MQ=255)
                                                                                           acggGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATg                                      >  1:257862/1‑143 (MQ=255)
                                                                                             gggggAAGGACGTGCGTCAGCGGGAAAGAAAGCGGTGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGt                                    <  2:255076/143‑1 (MQ=255)
                                                                                               gggAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTg                                  >  1:252473/1‑143 (MQ=255)
                                                                                                             tCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTAcc                    >  1:165522/1‑143 (MQ=255)
                                                                                                               ggcggGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGa                  >  1:472326/1‑143 (MQ=255)
                                                                                                                gcggGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTCACCGAt                 >  1:230885/1‑143 (MQ=255)
                                                                                                                gcggGAAAGAAAGCGGGGCGTGAACGACCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCCAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGACGAACGGATGGTTCACGACGAACTCAcccaa                 >  2:405030/1‑140 (MQ=255)
                                                                                                                 cggGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCGGGGCTGAAGAACGGAGAGTTGAACACGAAATTACGGATa                >  2:97675/1‑143 (MQ=255)
                                                                                                                  gggTAAGAAATCGGGGCGTGAACGCCCCGCGATTGAACATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATAc               <  1:480151/143‑1 (MQ=255)
                                                                                                                         atcgCGGGGCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGACGCAAACGGCCAGGGCAACGCCCAGAGTACCCGGGATGAAGAGCGGGTGGCTGAAGACGAATTTACCGGTACggggggt        >  2:346257/4‑139 (MQ=255)
                                                                                                                         aaagCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTCCCGATACGGgcagt        >  2:467096/1‑143 (MQ=255)
                                                                                                                             cGGGGCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCAAGAGAACCCCGCATGAAGAGCGGATGGTTGAGGACAAAACTAACGAGACTGGTAGCAccc    >  2:108519/1‑143 (MQ=255)
                                                                                                                               gggCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCgt  <  1:283980/143‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
ACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGT  >  NC_000913/4365319‑4365588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑