| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,365,453 | A→C | 12.1% | intergenic (‑97/+19) | cutA ← / ← dcuA | copper binding protein CutA/C4‑dicarboxylate transporter DcuA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,365,453 | 0 | A | C | 12.1% | 68.0 / 3.8 | 34 | intergenic (‑97/+19) | cutA/dcuA | copper binding protein CutA/C4‑dicarboxylate transporter DcuA |
| Reads supporting (aligned to +/- strand): ref base A (15/14); new base C (2/2); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-01 | |||||||||||
ACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCC > NC_000913/4365319‑4365595 | acGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTTCACGCCCcg < 2:519638/143‑1 (MQ=255)acGACAGACGCGGGATTCGAACTTTTTTCATCAAGCATAAACACCGCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTTCACGCCCcg < 1:130955/143‑1 (MQ=255) cgcgGTATGCGAACTTTTTTCATCAAGCAGAAACACCTCGCTAGGACAGATGGAACCGCAAGAGAGAGGGCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGtgattgat < 2:536265/143‑1 (MQ=255) gcgGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAGGAGAGAGGTCACTATACAACGGACGGGGGGAGGATGTTAGTAGGCGAGAAAGAAAGCGGGGCGTGGAAGCCTAGCGagcgagc > 2:98155/1‑137 (MQ=255) ttttttCATCAAGCGTAAGCACCTCATCAGCACAGATGGAACCGCAAGAGCGTGGTCACTATACAACGGACGGGGGAAGGATGTGAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGaa < 2:403063/143‑1 (MQ=255) tcaAGCATGAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCa < 2:529517/143‑1 (MQ=255) gCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGccc > 1:168997/1‑141 (MQ=255) tAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCAcgaa > 1:158407/1‑143 (MQ=255) tAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGACGTTAGTCGGCGGCAAAGAAAGCGGGGCGTGAACGCACCGAGATAGATCACTCCAGCACGACGCTACCCAacaccca > 2:485220/1‑140 (MQ=255) aaaCACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGACGCTACCCAGCGcgacg > 1:34820/1‑141 (MQ=255) acCTCGCGAGAGCGGATGGAACCGCAGGAGAGAGGGCACGATGCAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAGGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCAcgaagccga < 2:344367/143‑1 (MQ=255) cTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATGGATCATTACAGCATGAAGCTACCCAGCAcgaagccgaag > 1:330424/1‑143 (MQ=255) ataaCGGATGGAATCGCAAGAGAGAGGTCACTATACAAGGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGCCAGCGTGTCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACg < 2:496718/141‑1 (MQ=255) aCAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGAGTGATCATTACAGCATGAAGCGACCCAGCACGAAGCCGAAGCAAACGGcc > 2:519129/1‑143 (MQ=255) aCCGCAAGGGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAaca < 1:264235/143‑1 (MQ=255) agagGTCACTATACACCGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACAACCAGGGCAACACCCAGAGTAc > 2:453259/1‑143 (MQ=255) ggTCACTATAGAACGGACGGGGGAAGGCTGTTAGTCGGCCGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCg < 2:78491/143‑1 (MQ=255) catgaaaCAGCGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGa < 1:69952/137‑1 (MQ=255) cagtataAAACGGGGGGGGGAAGGGTGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGa < 2:168997/140‑1 (MQ=255) cACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGa < 1:116008/143‑1 (MQ=255) gacggacggGGGAAGGATGCTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAAc < 2:130886/142‑1 (MQ=255) acggGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATg > 1:257862/1‑143 (MQ=255) gggggAAGGACGTGCGTCAGCGGGAAAGAAAGCGGTGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGt < 2:255076/143‑1 (MQ=255) gggAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTg > 1:252473/1‑143 (MQ=255) tCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTAcc > 1:165522/1‑143 (MQ=255) ggcggGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGa > 1:472326/1‑143 (MQ=255) gcggGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTCACCGAt > 1:230885/1‑143 (MQ=255) gcggGAAAGAAAGCGGGGCGTGAACGACCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCCAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGACGAACGGATGGTTCACGACGAACTCAcccaa > 2:405030/1‑140 (MQ=255) cggGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCGGGGCTGAAGAACGGAGAGTTGAACACGAAATTACGGATa > 2:97675/1‑143 (MQ=255) gggTAAGAAATCGGGGCGTGAACGCCCCGCGATTGAACATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATAc < 1:480151/143‑1 (MQ=255) atcgCGGGGCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGACGCAAACGGCCAGGGCAACGCCCAGAGTACCCGGGATGAAGAGCGGGTGGCTGAAGACGAATTTACCGGTACggggggt > 2:346257/4‑139 (MQ=255) aaagCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTCCCGATACGGgcagt > 2:467096/1‑143 (MQ=255) cGGGGCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCAAGAGAACCCCGCATGAAGAGCGGATGGTTGAGGACAAAACTAACGAGACTGGTAGCAccc > 2:108519/1‑143 (MQ=255) gggCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCgt < 1:283980/143‑1 (MQ=255) aaCGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatcc < 2:184850/143‑1 (MQ=255) | ACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCC > NC_000913/4365319‑4365595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |