Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	754,446	A→G	100%	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	100.0%	80.1 / NA	26	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/18);  total (8/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGG  >  NC_000913/754308‑754580                                                                                                                                           |                                                                                                                                       tCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGttt                                                                                                                                    <  1:84673/143‑1 (MQ=255)   gCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTg                                                                                                                                  <  2:418439/143‑1 (MQ=255)             gctgaagtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGATTTTGCTTTTGTATc                                                                                                                        <  2:454959/141‑1 (MQ=255)              ttGAAGTGGAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCa                                                                                                                       <  2:331761/143‑1 (MQ=255)                        gCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                             <  1:272859/143‑1 (MQ=255)                                   aGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAgc                                                                                                  >  1:489847/1‑143 (MQ=255)                                        aaCACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCtt                                                                                             <  1:309727/143‑1 (MQ=255)                                        aaCACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCAGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCtt                                                                                             <  2:253228/143‑1 (MQ=255)                                               cTTTTGAACCGAGAGTACGGCTATCAATAACATCTGGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGt                                                                                      <  2:409488/143‑1 (MQ=255)                                                 tttGAACCGAGAGTACGGATATCAATAACATCTTGATTCAGCGACCCAACCAGAACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGCGGGTTTTGCTTTTGTATCATCCATTTAAGGTCTCCCTACCGCCTTCTTACTTaa                                                                                    >  2:526085/1‑143 (MQ=255)                                                     aaCCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTCGCGCCTTATTGCGTAAGACt                                                                                >  1:279069/1‑143 (MQ=255)                                                       ccGAGAGTACGGAAAACAATAACCTCTTGTCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGc                                                                              <  1:244879/143‑1 (MQ=255)                                                            agTACGGATATCAATAACATCTTGCCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGaa                                                                         <  1:405787/143‑1 (MQ=255)                                                                   atatCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAt                                                                  <  1:70805/143‑1 (MQ=255)                                                                        aaTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGCGGGTGGGTTTTGCTTTTGTATCAGCCATTTATGGTTTCGTTTGCGCCTTATTGCGTAAGACTGCCGGCACTTCCATTTGcc                                                             >  1:509051/1‑143 (MQ=255)                                                                                       cAGCGTGCCTGTCAGCTCATCCAGTTGAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCt                                              <  2:336937/143‑1 (MQ=255)                                                                                         gCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTCCGTAAGACTGCCGGAACTTATATTCGCCTTCCCGCATCAACCTgg                                            >  1:72839/1‑143 (MQ=255)                                                                                                     gCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGttt                                >  1:165053/1‑143 (MQ=255)                                                                                                     gCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGttt                                <  2:489847/143‑1 (MQ=255)                                                                                                           ccAGTTCAACAGCTGTAGCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTAtt                          <  2:6191/143‑1 (MQ=255)                                                                                                            cAGTTCGACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTAttt                         <  2:271051/143‑1 (MQ=255)                                                                                                              gTTCAACAGCTGTATCCCCGGTGGGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTgg                       <  2:426252/143‑1 (MQ=255)                                                                                                                             ccccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGa        >  1:39557/1‑143 (MQ=255)                                                                                                                               ccGTTGAGGGTGGGTTTTGCTTTTGTATCAGTCATTTAAGGTCTGTTTAGCGCCTTATTGCGTAAGACTGCCGGTACTTAAATTTGCCTTCGCACATCAACCTGGCTTCACCCGTTTTTTATTTGGCTCGCCCctcttcgatt      >  2:64752/1‑137 (MQ=255)                                                                                                                                cggtGAGGGTGGGTTTGGCTTTGGTATCAGCCATTTAAGGTCGCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAag     <  2:232663/140‑1 (MQ=255)                                                                                                                                   tGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAgg  <  1:326306/143‑1 (MQ=255)                                                                                                                                           |                                                                                                                                       TCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGG  >  NC_000913/754308‑754580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑