| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,074,945 | T→G | 18.1% | N1313T (AAC→ACC) | putA ← | fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,074,945 | 0 | T | G | 18.1% | 32.6 / 2.5 | 20 | N1313T (AAC→ACC) | putA | fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA |
| Reads supporting (aligned to +/- strand): ref base T (10/6); new base G (2/2); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
TTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCG > NC_000913/1074803‑1075087 | ttGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGCGGTTGTCACATCCTCCAGCTACCTGTTTAAACTATAGTCATTAAGCTGGCGt > 1:311052/1‑143 (MQ=255) gcggTATGTGTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcag < 2:15784/141‑1 (MQ=255) gAGGTATTTTTGGATCGGTCGGTTGGAATCGTGCGGCGGTTTTTTATTGAGGGGGTTCGCCCACGTTAAAGATGCCGGTGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcag < 2:189377/143‑1 (MQ=255) gggTATTTTTCAAACAAACAGAAAATAACGTGCCGGGGGTTTTCCCTTACTGCATTCGACCACGTTAAAGAATCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGGTAACGCcagc < 1:389577/142‑1 (MQ=255) gaTTGTTCATTCAATGGGTTGAAAACGTGCAGCGGATTATTAGGGAGGGGATTCGACCACGTTAAAGGTGGCGGAGGAGGTTGTAACGTCCTCCGGCTACCTGTTTAACCTATAGTCATTCAGCTGGCGGTAACGCcagctta < 2:90559/142‑4 (MQ=255) tcaaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCAGGAGGAGGTTGTAACGCGCTCCGGCTACCTGTTTAACCTATAGTCATTCAGCTGGCGGTACCGCCAGGGGCAGCGGTATTc > 2:62582/1‑143 (MQ=255) caaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGGTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTca > 2:17492/1‑143 (MQ=255) ttGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAg < 1:218844/143‑1 (MQ=255) cAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCAATAGTCATTAAACTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGa > 1:468155/1‑143 (MQ=255) attattGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTCTAGTCATTAAGCTGGCGTTACCGCCAGCGGCCGCGGTGTTCACACTCAGCGAACGCTCGATATACAGcc > 2:313758/1‑143 (MQ=255) gggATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCGTTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGCCCGATCGGGGCACGGCCGCTCCAGaa > 1:119404/1‑143 (MQ=255) gggATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGaa < 1:240893/143‑1 (MQ=255) cGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTACGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACACCCGTTCCCGCAGGATATTGCTTTCGCCACGGGCaa > 2:98183/1‑143 (MQ=255) cGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCACTCACACTCAGCGAACGCTCGACCTACCGCCGCTCCCGCAGGATCTTGCATCCGCCACGGGcca > 2:154070/1‑141 (MQ=255) tccGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACCCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGCTATTGCTTTCGCCACGCGCAACACCCTGCCCCGCAACAAtt > 2:484633/1‑143 (MQ=255) ccGGCTACCTGTTTCACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATtg < 1:398837/143‑1 (MQ=255) cGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATtgt < 1:515738/143‑1 (MQ=255) tttAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCGGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCcgc > 2:70519/1‑143 (MQ=255) aTTAAGCTGGCGTTACCGCCAGCGGCAGCGGTTTTCACACTCAGCGAACGCACGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTc > 2:281440/1‑143 (MQ=255) aTTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTCCGCCACAGGCAAGCCCCTGCAACGAACCTAGTGTTCCATCCCGCGCAGCAACttcctc > 2:494643/1‑138 (MQ=255) cTGGCGGTAACGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACaa > 1:515525/1‑143 (MQ=255) ttACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCACCTGCTTCACACAAGgcgcc > 2:419942/1‑142 (MQ=255) | TTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCG > NC_000913/1074803‑1075087 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |