| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,285,190 | T→A | 13.4% | L18I (TTA→ATA) | narJ → | nitrate reductase 1 molybdenum cofactor assembly chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,285,190 | 0 | T | A | 13.4% | 70.5 / 6.0 | 30 | L18I (TTA→ATA) | narJ | nitrate reductase 1 molybdenum cofactor assembly chaperone |
| Reads supporting (aligned to +/- strand): ref base T (13/13); new base A (2/2); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.34e-01 | |||||||||||
ATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGCGA > NC_000913/1285048‑1285329 | aTGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCt > 1:448324/1‑143 (MQ=255)aTGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCt < 1:278775/143‑1 (MQ=255) cACGGTGCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGgcag < 2:365242/143‑1 (MQ=255) gcGGTTCCGCTACCAAGTCCAAGCTGTTCACCCGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCATAAGgcagc < 2:423073/142‑1 (MQ=255) aCGGTTCAGATACCAAACTCAATCTGTTCCACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGgcagc < 1:467424/143‑1 (MQ=255) tCAGATACTAAATTCAGTCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCATAAGGCAGCATccg < 1:159833/143‑3 (MQ=255) cAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATcaac < 1:381807/143‑1 (MQ=255) ttCAACAGCCGTCGTATCGATGCCCTCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGAtt < 2:256091/143‑1 (MQ=255) aTCGATGCGATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAGCTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAAt < 2:233078/143‑1 (MQ=255) tGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGcc > 1:456465/1‑143 (MQ=255) aTCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGACGTTTGAGGCGATTGCCGCGTCGAAAACTCTGCCaaca > 2:53189/1‑141 (MQ=255) aGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCCCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACGAGAGATGTTTGAGGCGATTGCCGCGCCGAAAAACCTGCCAAAAGAGGATCCCCAt > 2:344254/1‑143 (MQ=255) cAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAAACTCGACAAAGAGGGTTCCCGTgc > 2:463133/1‑143 (MQ=255) ccGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCAtg > 1:429571/1‑142 (MQ=255) catccatcATCGAACTCGTGATTGTATCGCGCCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTc < 2:488672/143‑1 (MQ=255) cATCCATGATCGGACTCGTGATTGGATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTc < 2:352172/143‑1 (MQ=255) cATCCATGATCGAACTCGTGCTTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTc < 2:352169/143‑1 (MQ=255) cATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAACAATCTGCCAAAGGGGGATGCCCAGGCGCTGGGCATTCTCCGgc > 2:392692/1‑143 (MQ=255) tcaaccTCGTGATTGTACCGCGCCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGAt < 1:45865/138‑1 (MQ=255) cTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAac < 2:456465/143‑1 (MQ=255) cgcgagtGTAGCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGCTGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAacga < 1:217159/137‑1 (MQ=255) gTGATTGTATCGCGTCTACATGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATCAGCTGGGCCTGTACCTGCGCGATTTCacgac > 2:169898/1‑143 (MQ=255) tGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCCACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAacgacg > 2:297328/1‑143 (MQ=255) gATTGGATCGCGTCTCCTTGAATATCCGGAGGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGATGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAAcgacga < 1:184270/143‑1 (MQ=255) tCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAACCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTGAACGACGATGGATcc > 2:58772/1‑143 (MQ=255) ccTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCATGCGCGATTTAACGACGATGGATCCGCTCGATg > 2:22579/1‑143 (MQ=255) ttGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTGCTGCGCGATTTAACGACGATGGATCCGCTCGATGcc > 1:236387/1‑143 (MQ=255) gttgATGCTGCCATAAGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCa > 2:198416/4‑143 (MQ=255) gATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTa < 1:239835/143‑1 (MQ=255) gATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCGGGGCATTTTCCTGCGCGATTTAACGACGATGGACCCGCTAGATGCCCAGGCGCAGTa > 2:523138/1‑143 (MQ=255) aTGCTGCCATAAGGCAGCATCAACAAGAGATATCTTAGTCGTTTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCACTTTCCTGCGCGATTTAACGACGATGGATACGCTCGATGCCCAGGCGCAGTAc > 2:296122/1‑143 (MQ=255) gcCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCTTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGCTTTGTCGACGGTGGATCAGCTCGATGCCTAGGCGCGGTACAGccg > 1:171702/1‑141 (MQ=255) | ATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGCGA > NC_000913/1285048‑1285329 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |