| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 124,241 | C→T | Q409* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 124,241 | 0 | C | T | 94.7% | 140.9 / ‑5.4 | 59 | Q409* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (34/20); total (36/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGC > NC_000913/124104‑124373 | gtggtCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAgggt > 1:217069/1‑141 (MQ=255) tCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAATGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGa < 1:651100/143‑1 (MQ=255) aCGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTATTCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGGTAAGaaa > 2:1206780/1‑143 (MQ=255) aCGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGaaa > 2:721433/1‑143 (MQ=255) cgttCCGAAAAAAATCTACGCTGCATCCAATAAATCGCTGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGATTACGGCATGGGCGACGCGGCTGAATGTAAAAACATCGCGCACTAGGTTAAGaaaa < 2:375197/140‑1 (MQ=255) cGATCCGAAGAAAATCTACGCTGCAGTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGaaaa < 1:1121539/143‑1 (MQ=255) agaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATg > 1:424741/1‑143 (MQ=255) aaaTCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTACGTTAAGAAAATGAACATGGAc > 2:323208/1‑143 (MQ=255) aTCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCGCTAGGTTAAGAAAATGAACATGGACgg > 1:330817/1‑143 (MQ=255) tGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGta > 2:352668/1‑142 (MQ=255) tGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTc > 1:538842/1‑143 (MQ=255) tGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTc > 2:399315/1‑143 (MQ=255) tGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAACGAACATGGACGGTGTGCGTc > 2:353308/1‑143 (MQ=255) ttCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCatat > 1:1217497/1‑143 (MQ=255) aaGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAGGAAAATGAACATGGAGGGTGTGCGTCATATcgg > 1:530749/1‑141 (MQ=255) aGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGCCataacagc > 2:676648/1‑138 (MQ=255) cgcAGGAAACCAAAGGCAAAGCGACAGTAATGCGAGCGCAGACCATTAAGGGTTACAGCACGAGCGCCTACGCTGAAGGTCAAAACATCGTGCACCAGGGAAAGAAGATGAAAAAGCACGACGTGCGGAATATCGGCGCCCga > 2:1193693/1‑142 (MQ=255) gcAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTCGGTTTCGAACTTGAACATGGACGGTGTGCTTCATATCCGCGACCGtt > 1:44220/1‑143 (MQ=255) taGGTAACCAAAGGAAAGGCGACAGTAATCCGTGCTCAGACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGCCACATGCAGCCACTTTGACGGTGTGCGTCATATCCGCGACCGttt < 1:1177524/142‑1 (MQ=255) cAGGGAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGttt < 2:486569/143‑1 (MQ=255) ggAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGCACATGGACGGTGTGCGTCATATCCGCGACCGTTTCa > 1:483285/1‑143 (MQ=255) gAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGCCGACGCGACTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTcca > 1:840699/1‑141 (MQ=255) aaaCCAACGGCAAATCGACAGTAATCCTGGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAAt < 2:760547/143‑1 (MQ=255) ccAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATAGCCGCGACCGGTTCAAtgtg > 1:1073207/1‑143 (MQ=255) cAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGACGGTAAAAGCATCGCGCACTAGGGTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGc > 1:1106474/1‑143 (MQ=255) cAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGc > 1:908497/1‑143 (MQ=255) cAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCAGCGACCGCTTCAATGTGc > 1:1183410/1‑143 (MQ=255) aaaGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGcc > 1:784690/1‑143 (MQ=255) ggCAAAGCGACACTAATCCTTGCTCATACCATTCAAGGCTACGGCATGCGCGACGCGGCTCAAGGTAAAAACACCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGTCCGTTTCAATGTGCCGgt < 1:678243/143‑1 (MQ=255) aaaGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCACATCAATGTTCCAGTGTc > 1:766188/1‑143 (MQ=255) aaaGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGGTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTc > 1:939236/1‑143 (MQ=255) gaccGACAGCGATCCTTGCTCAGACCGTTAAGGGTTCCGGCATGGGCGACGCGGCTGAAGGTAAGAACATCGCGCACTAGGTTAAGAGAATGAACATGGCCGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCt < 2:534064/140‑1 (MQ=255) cAGTAATCCTTGCTCATACCCTTAAACGTCACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCCCTAGGTTACGAAAATGAACATGGACGGTTTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCa < 1:198583/143‑1 (MQ=255) tCATACCATTAAAGGTTACGGCCTGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGATAATGAACATGGACGGTGGGCGTCCTACCCGAGACCTCGTCCAGGTCCCGGGAGCTGCTGCAGAGATCGCAAAAc > 2:405851/1‑143 (MQ=255) tCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGCAGGTAAAAACATCGCGCACTAGGTTAAGAAAAGGAACATCGACGGTGTGAGTCATATCCGCGACCGTATCAATGTGCCGGGGTGTGATGCAGATATCGAAAGAc > 2:410987/1‑143 (MQ=255) tCATACCATGAAAGGTTACGGCATGGGGGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAAc < 2:222853/143‑1 (MQ=255) aCCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATCTCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGcc > 1:809930/1‑143 (MQ=255) aCCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAGACTccc > 1:218821/1‑143 (MQ=255) aCCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGACGATATCCAAAAACTGcc > 2:521751/1‑143 (MQ=255) ccATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCg < 2:45943/143‑1 (MQ=255) ccATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCg < 2:424741/143‑1 (MQ=255) ttAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTAc < 1:1120685/143‑1 (MQ=255) gTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGGTATCGAGAAACTGCCGTCCATCAcc > 1:228121/1‑143 (MQ=255) ttACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGGTAAGAAAAGGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATTTGCCGGTGTCTGATGCAGATGTCGAAAACCTGCCGTACCTCCCCt > 1:775789/1‑143 (MQ=255) gCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCg > 1:1205573/1‑143 (MQ=255) tGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGaa < 1:399315/143‑1 (MQ=255) cgggCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGaa < 2:1153902/142‑1 (MQ=255) gACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTc > 1:865378/1‑143 (MQ=255) aCGCGGTTGAAGGTAGAAACGTGGCGCACGAGGTTAAGAAAATGAACATGGACGGGGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAACACTGCCGTACATCACCTTCCCGGAAGGTTCt < 2:330856/143‑1 (MQ=255) ggcggcgGAAGGGAACAAAGTCGCGCAGCAGGGGAAGAAAATGACCATGGACGGTGTGCGTCATATCCGCGACCGTTTCAAGGGGCCGGTGTCGGATGCAGATATCGAAAAACCGCCGTACATCACCTTCCCGGAAGGTTCTg < 1:151836/142‑1 (MQ=255) cgGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGGAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGaa > 2:846326/1‑143 (MQ=255) gCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAaga < 2:330817/143‑1 (MQ=255) tGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTTCCGTACATCACCTTCCCGGATGGTTCTGacgtgc > 1:24710/1‑138 (MQ=255) aGGTAAACACATCGCGCACTAGGTTAAGAAAATGAACCTGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATa < 1:472074/143‑1 (MQ=255) cttAAAAGCATCGCGAACTAGGTTAAGAAAAGGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATAc < 1:1089302/141‑1 (MQ=255) tAAAAACATCGCGAACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCTGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCt < 1:173052/143‑1 (MQ=255) aaaaaCATCGCGCACAAGGTTAAGAAAATGAACATGGACGGTGTGCGTAATATCCGCGACCGTTTCACTGTGCCGGTGTCTGATGCAGATATCGGAAAACTGCCGTGCATCACCTTCCCGCAAGGCTATGACGAGCagcgagc > 2:20976/1‑137 (MQ=255) aaCATCGCGCACTCGGTTAACAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTAAATCTCCGTCCCGGAAGGTTCTGAAGAGCATACCTATCt < 2:380660/143‑1 (MQ=255) ccgcgcgCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGGCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGc < 2:851780/140‑1 (MQ=255) | GTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGC > NC_000913/124104‑124373 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |