Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,661,821 C→G C104S (TGC→TCC)  iscR ← DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,661,8210CG100.0% 151.9 / NA 49C104S (TGC→TCC) iscRDNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (26/23);  total (26/23)

GTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACG  >  NC_000913/2661687‑2661957
                                                                                                                                      |                                                                                                                                        
gTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATcg                                                                                                                                  <  2:180460/143‑1 (MQ=255)
 tGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGATCGCCTAACGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGCTCAAGGATATAgcgc                                                                                                                                 >  2:189713/1‑143 (MQ=255)
  cgcgcGTCCGAAGGATCCTGACGACCAGACACATCCAGCACTTCCCGGTTATTAACCAGTTCGCCTAAGTTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATcgcc                                                                                                                                <  1:484856/142‑1 (MQ=255)
   gcgcGTCGTGAGTATGCTGACGACCCGACACATCCAGCACCTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATcgccg                                                                                                                               <  1:1112495/143‑1 (MQ=255)
     gcgTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAGAACCGGTGAGACGGTCACTTAACTCACGCCACCATGCCTCGGCCCGGGATTTATcggcaca                                                                                                                             >  2:69928/1‑138 (MQ=255)
               tATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAgctt                                                                                                                   >  1:759259/1‑141 (MQ=255)
               tATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAgccg                                                                                                                   >  1:790222/1‑143 (MQ=255)
                gtGCTGACTACCAGCGACATCCCGCCCTTCCTGTTTATTACCCAGTTCGCCTAAAGTAATGTTGTTGAGAAATCCGGTGCGACGGGCGCGCAACTCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAgccgc                                                                                                                  <  2:836424/142‑1 (MQ=255)
                       cgacCAGACACATCCAGCACTTCCTGGTTATTACCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAcc                                                                                                           <  1:112753/143‑1 (MQ=255)
                        gacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTACAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGGAGGGATTTATCGCCCCCCTGGCAGCCGCCTTTAccc                                                                                                          >  2:1095185/1‑143 (MQ=255)
                            aGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGAc                                                                                                      >  1:761074/1‑143 (MQ=255)
                             gACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACa                                                                                                     >  1:878655/1‑143 (MQ=255)
                                 caTCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACgg                                                                                                 <  1:63780/143‑1 (MQ=255)
                                  aTCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACggg                                                                                                >  2:662268/1‑143 (MQ=255)
                                   tCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACCGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATGTATCGACGCCCTGGCAGCCCACTGCAACCTGACAACggga                                                                                               >  2:166569/1‑142 (MQ=255)
                                      aGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGc                                                                                            >  2:597889/1‑143 (MQ=255)
                                           ttCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTCTCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGCGTGGCATCTa                                                                                       >  2:553086/1‑143 (MQ=255)
                                             ccTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACa                                                                                     >  1:803582/1‑143 (MQ=255)
                                                      taACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGGATTTATCGCCGCCCTCGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCa                                                                            >  1:754049/1‑143 (MQ=255)
                                                       aaCCAGTTCGCCTAAAGTAATGTTGTGGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCaa                                                                           <  1:56187/143‑1 (MQ=255)
                                                        aCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAAc                                                                          >  2:308968/1‑143 (MQ=255)
                                                         cgaGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGGGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCGACAGCTCCGTCAACg                                                                         >  2:214394/3‑143 (MQ=255)
                                                          cAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCCGGGCTTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATACGTCACCgg                                                                        >  2:637071/1‑143 (MQ=255)
                                                          cAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACgg                                                                        >  2:125328/1‑143 (MQ=255)
                                                          cAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACgg                                                                        >  1:855632/1‑143 (MQ=255)
                                                           aGTTCGCATAACGTAATGTTCTTGCGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGgc                                                                       <  2:989563/143‑1 (MQ=255)
                                                                gCCTAAAGT‑ATGTTGTTGAGAAAACCGGGGAGACGGTCGCCCAAATCACCCCACCGCGCGTTGGGCAGGGGATTATCGCCGCCCCGGCCGCCGCCCTTACCCTGACCACCGGTGGCCTCCACCGGTTCGTCCACGgcgccgat                                                                 >  1:1005599/1‑139 (MQ=255)
                                                                  cTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGACAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTACtt                                                                >  1:204935/1‑143 (MQ=255)
                                                                   tAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACCGATTCGTCAACGGCGCTAATTa                                                               >  1:943548/1‑143 (MQ=255)
                                                                   tAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGCTTCGTCAACGGCGCTAATTa                                                               >  1:439743/1‑143 (MQ=255)
                                                                      aGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACtt                                                            <  1:662503/143‑1 (MQ=255)
                                                                       gTAATGTTGTTGAGAAAGCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTc                                                           <  2:219653/143‑1 (MQ=255)
                                                                       gTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTc                                                           <  2:761074/143‑1 (MQ=255)
                                                                              tgtctataaatCCGTTGAGACGCTCGCTCATATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGTGTGGCATCTACAGATTCGTCAACTGCGCTAATTACTTCGCCAACg                                                    <  2:463314/136‑1 (MQ=255)
                                                                                   agaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGGCGAGGGCGAt                                               >  1:686936/1‑143 (MQ=255)
                                                                                       aaCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAtgctg                                           <  1:597889/143‑1 (MQ=255)
                                                                                          cGGTGAGCCGGTCGCTCAGATCACGCCACAGCGCGTGGGTCAGCGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGctgctg                                        <  1:1105759/143‑1 (MQ=255)
                                                                                           ggTGCGCCGGTCGCTCCGACCACGCCTCGTCGCGTGGGTCAGGGGTTTATCGCCGCCCTGGCAGCCGCCGTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTgg                                       <  2:642321/143‑1 (MQ=255)
                                                                                            gTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCTGCCTTTACCCTGACCTTGGGTGGCAGCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGc                                      <  2:878655/143‑1 (MQ=255)
                                                                                             tGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTCCGCCAACGGCGATGCTGCTGGCa                                     >  1:573697/1‑143 (MQ=255)
                                                                                                    gTCGCTCAAATCACGACACAGCGCGTGGGTCAGGGATTCATCGCCGCCATGGCAGACGCCTTTCGCCTGCCAACGGGTGGCATCTACAGATTCGTCCACGGTACGTTTTACGTCGCCAACGGCGATGCTGCTGGCATCTTTGc                              <  2:606100/143‑1 (MQ=255)
                                                                                                     tCGCTAAAATCACGCCACAGCGCGTGGCTCACGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGcc                             <  2:555455/143‑1 (MQ=255)
                                                                                                        cgaacaacACGGCACAGCGCGTGGGTCAGGGATTTAGCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGCGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTaa                          <  2:795899/136‑1 (MQ=255)
                                                                                                         tCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAAc                         <  1:610509/143‑1 (MQ=255)
                                                                                                                     acaGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCg             <  1:812725/143‑1 (MQ=255)
                                                                                                                          gcgTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTgg        <  1:1225155/143‑1 (MQ=255)
                                                                                                                            gTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCGTTTACACTGACAACGGGAGCCATCTACAGATTCGGCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTCGCATCGTTGCCTAACAGATAACCCGCGccccggc      >  2:1095819/1‑138 (MQ=255)
                                                                                                                               ggTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCAc   >  1:1184976/1‑143 (MQ=255)
                                                                                                                                gtgaGGGATTTATCGCCGCGCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACg  <  1:355922/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                        
GTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACG  >  NC_000913/2661687‑2661957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑