Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,634	A→C	T108P (ACC→CCC)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,634	0	A	C	100.0%	195.5 / NA	66	T108P (ACC→CCC)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (30/36);  total (30/36)

CCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATG  >  NC_000913/3041495‑3041770                                                                                                                                            |                                                                                                                                         cccttGCGACGCCACAGGTAAAATGCGTAGCAATTTACGGCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGGGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCa                                                                                                                                       <  2:721272/140‑1 (MQ=255)       agACGGCAAAGGGAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGACTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTAGTCTCTAAAGTGCCCATcgcgg                                                                                                                                 >  2:824891/2‑142 (MQ=255)          cGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAg                                                                                                                              <  1:26043/143‑1 (MQ=255)            ccAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGACGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGAATTCCCTAAAGTGCCCATCGCGCAAgac                                                                                                                            >  2:812696/1‑143 (MQ=255)             cAAAGGTAAAATGTGGAGCAATTTACGTCTGTGCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAACAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAgacg                                                                                                                           <  2:240398/143‑1 (MQ=255)              aaaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAgacga                                                                                                                          >  2:903190/1‑143 (MQ=255)                  gTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAg                                                                                                                      >  1:314189/1‑143 (MQ=255)                   tAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAACAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGc                                                                                                                     >  2:367398/1‑143 (MQ=255)                     agaTGTGGAGCAAGTTACGCCGGTGCCGCGGCGGCGATGGCTGTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCgt                                                                                                                   <  2:895583/141‑1 (MQ=255)                     aaaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGAGGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCgt                                                                                                                   <  1:586067/143‑1 (MQ=255)                          tgGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGtgct                                                                                                              >  1:578748/1‑143 (MQ=255)                           ggAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGtgctg                                                                                                             >  1:144749/1‑143 (MQ=255)                            gAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATCCTCAGCCGAGCCCATCGCGCCAGACGACGAGCGTGtgctgc                                                                                                            >  2:869918/1‑143 (MQ=255)                                  tttACGTCTGTTCCGCGACGTCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtg                                                                                                      <  2:62306/143‑1 (MQ=255)                                    tACGTCTGTTCCGCGACGGCGATCGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGtt                                                                                                    <  1:376082/143‑1 (MQ=255)                                     aCGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTg                                                                                                   <  2:1114211/143‑1 (MQ=255)                                       ggcggggccGAGACGGCGATGGCAGTGCAGGGAATGAACGGCGCAGCGTGCGTGAACCGAAGCGGACTGAACTGAAAAAATAGGCGGTATTCTCTAAAGCGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGGTGcc                                                                                                 <  1:264567/136‑1 (MQ=255)                                        tCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg                                                                                                <  1:903190/143‑1 (MQ=255)                                              tctcgACGGTTATGGCTTTGCATGGATTGAACGGCGCAGCGGGCGTGAACCGCAGCTGACTGAACTGAAAAAACATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                                                                                          <  1:66065/140‑1 (MQ=255)                                              ccGCGACGGGCATGGCGTTGCATGGATTGAGCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAACAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                                                                                          <  2:964255/143‑1 (MQ=255)                                                gcgagcgCGATGGCTTTGCATGGATTCAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAg                                                                                        <  1:492234/137‑1 (MQ=255)                                                gcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGAGAACCGCAGCTGACTGAACTGAAAAAATGTGCGGGATTCTCTAAAGGGCCCATCGCGCCAGCGGGCGGGGGTGGGCTGCTTGGGGATGCCGGTTGTCAg                                                                                        >  1:32185/1‑143 (MQ=255)                                                       tgATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcg                                                                                 <  1:930017/142‑1 (MQ=255)                                                            gCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGACAAAATATGCGGTATCCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCGGCTTGGTGCTGCCGGCTTTCAGGCGcgcgccccg                                                                            >  2:238632/1‑143 (MQ=255)                                                            gCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAACTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgcg                                                                            <  1:493603/143‑1 (MQ=255)                                                              tttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCt                                                                          >  1:18634/1‑143 (MQ=255)                                                                tGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTgg                                                                        <  1:990872/143‑1 (MQ=255)                                                                  cATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGGATTCGCTAAAGTGCCCATCGCGCCAGACGCAGAGAATATGATTCTTGGTGTTACCGGTTCTCAGGCGCGCGCCGCGCTGGCa                                                                      >  2:546116/1‑143 (MQ=255)                                                                   aTGGATTGAACGGCGCAGCGTACGTGAACCGCAGCTGACTGAACTGAAAAAACATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTCCCTGGTCTTGCCGGCTTCCAGGCGCGCGCCGCCCCGGCac                                                                     >  2:15774/1‑142 (MQ=255)                                                                       aTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct                                                                 >  1:289738/1‑143 (MQ=255)                                                                       aTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct                                                                 <  2:840809/143‑1 (MQ=255)                                                                        ttGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTACAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTGTCAGGCGCGCGCCGCGCTGGCCAAtcgc                                                                >  2:1136132/1‑141 (MQ=255)                                                                           aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt                                                             >  1:180029/1‑143 (MQ=255)                                                                            ccGGCGCACCGTCCCTTAACCGCCGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa                                                            <  1:786957/142‑1 (MQ=255)                                                                            aCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa                                                            <  1:776234/143‑1 (MQ=255)                                                                             cGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAg                                                           >  2:1071955/1‑143 (MQ=255)                                                                               gcgcATCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCg                                                         <  2:1021470/143‑1 (MQ=255)                                                                               gcgcAGCGGGCGGGAACCGCAGCTGACTGAACTGAGAAAATATGCGGTATTCTCTAAAGTGCCCAGCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCg                                                         <  2:233649/143‑1 (MQ=255)                                                                                 gcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCACCAGACGACGAGCGTGTGCTGATTGGTATTGCCGGAATTCAGGCGCAACCCGCGACGGCAAATCACATTAGCGaa                                                       >  2:13229/1‑143 (MQ=255)                                                                                  cAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAAc                                                      <  1:753585/143‑1 (MQ=255)                                                                                            gAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGCACTGCCTTCgaa                                            >  1:94657/1‑143 (MQ=255)                                                                                             taCCGCAGCTGGCTGGACTGAAAAAATATTCGGGATGCTCTGAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAGCTCTTTAGCGATCTGCCTTCgaaa                                           <  2:904753/142‑1 (MQ=255)                                                                                             aaCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGGCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa                                           <  2:314189/143‑1 (MQ=255)                                                                                                cGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaagaa                                        <  2:106274/143‑1 (MQ=255)                                                                                                    gCTGACTGAACTGAAAAAATATGCGGTATGCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAAc                                    <  2:349137/143‑1 (MQ=255)                                                                                                     ctcactgaactgaGAATATATGCGGTATTCCCTAATGTGCCCCGCCGGTGTGACGAAATACATGTGGTGCTTGGTGTTGCCGGTTCCCAGGCGGGTGCCGCTCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACa                                   <  2:927298/140‑1 (MQ=255)                                                                                                      tgactgaACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCTCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAg                                  <  1:1183303/143‑1 (MQ=255)                                                                                                          tgaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGGTTTCAGGCGCGCGCCGCGCCGGCAAGCCTCTGTCGCGAACTGCCTTCGAAAGAAAAACAGGTAg                              >  2:1066387/1‑143 (MQ=255)                                                                                                          tgaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGATTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCCATGCTCTTTAGCGGACTGCCTTCGCAAGACAAACAggcgg                              >  2:197677/1‑140 (MQ=255)                                                                                                             actgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGATGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCa                           <  2:144749/143‑1 (MQ=255)                                                                                                               tgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaaa                         <  2:1090611/143‑1 (MQ=255)                                                                                                                 aaaaaaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCGTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAAATGCCTTCGAAAGAAAAACAGGCCGTCAaaga                       >  2:804345/1‑143 (MQ=255)                                                                                                                  aaaaaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTAGTAAGCAAAACAGGTAGTCAaagca                      >  1:513079/1‑141 (MQ=255)                                                                                                                   aaaaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaag                     >  1:259897/1‑143 (MQ=255)                                                                                                                   aaaaTATGCGGTATGCTCTAAATTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTCAGCGAAATGCTTTCGAAAGAAAAACAGGTAGTCAaagaag                     <  2:751249/143‑1 (MQ=255)                                                                                                                     aaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgc                   <  1:1071955/143‑1 (MQ=255)                                                                                                                      atatGCGGTCTTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgcg                  <  1:262186/143‑1 (MQ=255)                                                                                                                         tGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGa               <  2:774048/143‑1 (MQ=255)                                                                                                                               aTTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACtt         >  1:359085/1‑143 (MQ=255)                                                                                                                                 tctcTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTg       <  2:359085/143‑1 (MQ=255)                                                                                                                                   tctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAACACAGGTAGTCAACGAAGGCCCGACCACTTTGCt     >  2:387720/1‑143 (MQ=255)                                                                                                                                   tctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAGGCAGGCGCGACCACTTTGCt     >  1:1157759/1‑143 (MQ=255)                                                                                                                                   tctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTCTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGCACTCCCCTCGCAAGACAAACAGGCAGTCCACGAAGCCGCCACCACTCTGCt     >  1:1154877/1‑143 (MQ=255)                                                                                                                                   tctAAAGTGCCCATCGCGCCAGACGACGAGCGTCTGCTGCTTGGTGTTGCCGGTCTTCAGGCGCGCACGGAGCTGGCAGATCTCTTCAGCGCCCCGCCCTCCAAAGAAACAGAGGACGTCCAAGAAGGCGCGACCACTTCGCt     >  2:204234/1‑143 (MQ=255)                                                                                                                                     tAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTAt   <  1:354458/143‑1 (MQ=255)                                                                                                                                      aaaGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAGCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGGTATg  >  1:180598/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                         CCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATG  >  NC_000913/3041495‑3041770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑