Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,446	A→G	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	96.8%	181.1 / NA	64	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (30/31);  minor base C (2/0);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGA  >  NC_000913/754310‑754583                                                                                                                                         |                                                                                                                                          gCAGGATGCGGTTGCAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTGGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTg                                                                                                                                     <  1:963360/143‑1 (MQ=255)  cAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACGCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGc                                                                                                                                    >  1:317856/1‑143 (MQ=255)     gATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGATCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCttt                                                                                                                                 >  1:346986/1‑143 (MQ=255)     gATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGGTTTGCttt                                                                                                                                 >  2:653229/1‑143 (MQ=255)      aTGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtttt                                                                                                                                <  1:505749/143‑1 (MQ=255)      aTGCGGTTGAAGTGAAGCCGGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtttt                                                                                                                                <  2:480208/143‑1 (MQ=255)        gCGGTTCCAGTGAAGCCTGGGTCAAAGGTGAACACTCCTTTTGAACCGAGAGTACGGATATCAATAACGTCTTGACCCACCGGGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGt                                                                                                                              <  2:813175/143‑1 (MQ=255)         cGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTa                                                                                                                             >  2:465698/1‑143 (MQ=255)          ggTTGAAGTGAAGCCTGGCTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTAt                                                                                                                            <  1:740162/143‑1 (MQ=255)            ttGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCa                                                                                                                          <  2:777923/143‑1 (MQ=255)                ggtgaagCGTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCa                                                                                                                      <  2:272164/142‑1 (MQ=255)                    aagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTa                                                                                                                  >  1:879342/1‑143 (MQ=255)                      ggctaggTCAAATGTGAACATCTCTTTTGCACCGTGAGTACGGATATCAATAACATCGTGACGCAGCGTGCCTTTCAGCACATCCAGGTCAACAGCTGTATCCACGTTGAGGGTGTGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                                <  1:158360/138‑1 (MQ=255)                       ccTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGCGGGTGCGTTTTGCTTTTGTATCAGCCATTTAAgg                                                                                                               >  2:691459/1‑143 (MQ=255)                         tGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGtc                                                                                                             >  1:820906/1‑143 (MQ=255)                         tGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGtc                                                                                                             >  1:754474/1‑143 (MQ=255)                           ggTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGtctc                                                                                                           <  2:887614/143‑1 (MQ=255)                             tCAAAGGTGAATACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCt                                                                                                         <  2:769206/143‑1 (MQ=255)                             ccAAAGGTGCACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCt                                                                                                         <  2:474093/142‑1 (MQ=255)                                      aaCTCACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTTCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCtt                                                                                                <  1:272669/143‑1 (MQ=255)                                        cacacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTAt                                                                                              >  1:578361/1‑143 (MQ=255)                                         acacCTTTTGAACCGAGAGTACGGATCTCAATAACATCTTGACCACGCGTGCCTTTCAGGATATCCAGTTCAACTGCTGTCTCCGCGTTGAGGGCGGGTTCTGCTTTCGTATCAGCCTTCTAAGGTCTCCGTCGCGCCtactt                                                                                             >  2:387070/1‑139 (MQ=255)                                           gcttttgGAACTGTGAGTGCTGATATCAATAACAGCTTGGCCCAGCGTGCCTTTCAGCACATCCAGGTCAACAGCTGTATCCCCGTTGAGGGTGGGTTGTGCTTGTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGc                                                                                           <  2:902158/140‑1 (MQ=255)                                           acCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGc                                                                                           >  1:543650/1‑143 (MQ=255)                                              ttttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTCAGGTCTCCTTAGCGCCTTATTGCGTa                                                                                        >  1:1104005/1‑143 (MQ=255)                                               tttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTaa                                                                                       <  1:660576/143‑1 (MQ=255)                                               tttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTaa                                                                                       <  2:169532/143‑1 (MQ=255)                                                 tGAACCGAGAGTACGGATATCAATAACATCTTGGCCCAGCGTGCCTTTCAGAACATCCAGTTCATCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGa                                                                                     <  2:511991/143‑1 (MQ=255)                                                    aCCGAGAGTACGGATATCAATAACATCTTGACCCAGAGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTCTCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGCCTg                                                                                  >  1:781192/1‑143 (MQ=255)                                                      cGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCGCCGTGGAGGGTGGGTTTTGCTTTTGTATCACCCATTTAAGGTCTCCTTAGCGCCTGATTGCGTAAGACTGcc                                                                                >  2:652035/1‑143 (MQ=255)                                                          cgTACGGATATCAATAACATCGTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGaa                                                                            <  2:836253/142‑1 (MQ=255)                                                          agTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCCCATCCCGTACAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGaa                                                                            <  1:108087/143‑1 (MQ=255)                                                               ggATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTCGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGCCTGCCGGAACTTaa                                                                       >  2:1225229/1‑143 (MQ=255)                                                                 atatCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGATTATGCATTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAt                                                                     >  2:433776/1‑143 (MQ=255)                                                                   atCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAttt                                                                   <  2:447676/143‑1 (MQ=255)                                                                     cAATAACATCTTGACCCAGCGTGCCTTTCGGCTCTGCCACCCCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGc                                                                 <  2:543650/143‑1 (MQ=255)                                                                     cAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTTCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGc                                                                 <  1:422329/143‑1 (MQ=255)                                                                       aTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCGCGGTGAGGGTGGGTGTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTCGCGCCTTGTTTCGTAAGACTTACGGAACTTCACtttttct                                                               >  2:854904/1‑139 (MQ=255)                                                                         aaCATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTATGTATCAGCCTTTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTc                                                             <  1:465698/143‑1 (MQ=255)                                                                          aCATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCCTTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAGCTGCCTTCg                                                            >  2:93391/1‑143 (MQ=255)                                                                          aCATCCTGACCCAGCGTCCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCg                                                            <  1:918384/143‑1 (MQ=255)                                                                           cATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCCTTTGTATCAGCCCTTTAAGGTCTCCTTCGCGCCTTCTTGCGTAAGGCTGCCGGACCTTAACTTTGCCTTCGc                                                           >  1:302844/1‑143 (MQ=255)                                                                                tGATCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATc                                                      <  2:346986/143‑1 (MQ=255)                                                                                   cccAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAAc                                                   >  1:652943/1‑143 (MQ=255)                                                                                     cAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCt                                                 >  1:149894/1‑143 (MQ=255)                                                                                     cAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAGTTGCCTTCGCACATCAACCt                                                 >  1:835008/1‑143 (MQ=255)                                                                                     cAGCGTGCCTTTCAGCACATCCAGTTCAACAGCGGGAGCCCCGTTGAGCGTGCGTTGTGCGACTGTACCAACCATTCAGGGTCTCCTTAGCGCCTTATTGCGTACGACTGCCGGGACTTGAATTTGCGTTCGCACATCAACCt                                                 >  2:261793/1‑143 (MQ=255)                                                                                            ccTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCGGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCCTCGCACATCAACCTGGCTTTa                                          >  2:1086491/1‑143 (MQ=255)                                                                                            ccTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCAGGATCTTGAAGGTGACTTCGCACATCAACCTGGCTTTa                                          <  2:1212130/143‑1 (MQ=255)                                                                                             cTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTTAGACTCCCGGAACTTAAATTTACCTTCGCACATCAACCTGGCATTaa                                         >  1:105099/1‑142 (MQ=255)                                                                                             cTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCTCTCATCAACCTGGCTTTAc                                         <  2:754474/143‑1 (MQ=255)                                                                                              tttCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCGTATTGCGTAAGTCTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAcc                                        >  2:332791/1‑143 (MQ=255)                                                                                                   gCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGttt                                   <  1:215421/143‑1 (MQ=255)                                                                                                    cacaTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtttt                                  >  1:841662/1‑143 (MQ=255)                                                                                                        tCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTAt                              >  1:687649/1‑143 (MQ=255)                                                                                                         ccAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTAtt                             >  2:46888/1‑143 (MQ=255)                                                                                                          cAGTTCCACAGCTGTATCCCCGTTGAGGGTTGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTAttt                            <  2:1112618/143‑1 (MQ=255)                                                                                                          cAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAGGACTGCCGGCACTTAAATTTGCCTTCGCAAATTAACCTCGCATTACCCGTTTTTTGttt                            >  2:539687/1‑143 (MQ=255)                                                                                                          cAGTTCAACAGCTGTACCCCCGTTGAGGGTGGGTTTTGATAATATTTCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTAttt                            <  1:697129/143‑1 (MQ=255)                                                                                                           aGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAACGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGGTTTACCGGAGTTTTATTTg                           >  1:438004/1‑143 (MQ=255)                                                                                                            gtctaaCAGCTGTATCTCCTTTGAGGGTGGGTTGTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTgg                          <  2:541423/139‑1 (MQ=255)                                                                                                                   aGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgccg                   >  1:812638/1‑143 (MQ=255)                                                                                                                             ccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCCGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGaaa         <  2:1104005/143‑1 (MQ=255)                                                                                                                                 tGAGGGTGGGTTGGGCTTTTGTATCAGCCCTTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCGACCTGGCTTTGCGCGTTTTTTATTTGACTCGCCGCTCTGTGAAAGAgg     <  2:60164/143‑1 (MQ=255)                                                                                                                                    gggtgggtTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGa  >  2:132834/1‑143 (MQ=255)                                                                                                                                         |                                                                                                                                          GCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGA  >  NC_000913/754310‑754583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑