Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,576,033 T→C 9.8% V529V (GTA→GTG)  yddB ← putative TonB‑dependent receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,576,0330TC 9.8% 97.2 / 3.4 41V529V (GTA→GTG) yddBputative TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base T (21/16);  new base C (2/2);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

TGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAG  >  NC_000913/1575891‑1576171
                                                                                                                                              |                                                                                                                                          
tGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGAt                                                                                                                                            >  1:107371/1‑143 (MQ=255)
   tttGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCACCGTTCTCGGGCGCCTTCAGGTCCTGATAACGCGTCAGAGTTTTATTACCTCATAcc                                                                                                                                         >  2:530546/1‑143 (MQ=255)
    gtGATCGTGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCg                                                                                                                                        <  1:69640/142‑1 (MQ=255)
      ggtCATGCGCTTCACGGTAGGCATAGTGTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGAt                                                                                                                                      <  2:131939/141‑1 (MQ=255)
      gATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGAt                                                                                                                                      <  1:144504/143‑1 (MQ=255)
                   aCGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGACACCGATTCCGTCCAGCTAt                                                                                                                         >  1:52212/1‑143 (MQ=255)
                   aCGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGACACCGATTCCGTCCAGCTAt                                                                                                                         >  1:436719/1‑143 (MQ=255)
                     ggTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTCTTg                                                                                                                       >  2:68507/1‑143 (MQ=255)
                       tAAACATCGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCg                                                                                                                     <  2:171939/143‑1 (MQ=255)
                           cATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGata                                                                                                                 >  2:367869/1‑143 (MQ=255)
                             tAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATCGCCAGTACATCGTTATAACGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCCGCTATAGGGGttatg                                                                                                               >  2:522533/1‑142 (MQ=255)
                               gTTTGTACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCAc                                                                                                             <  2:146434/143‑1 (MQ=255)
                               gTTTGCACGCGCAGTGACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCAc                                                                                                             <  2:353308/143‑1 (MQ=255)
                                    cACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATCCCGATTCCGTCCAGCTATTGCGGACATCACGTAAt                                                                                                        >  2:495393/1‑143 (MQ=255)
                                                         cGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGGGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCACATATGAAGAATAGTcgcc                                                                                   >  1:243450/1‑140 (MQ=255)
                                                          gATTTTCTTCTTCAATCCCATTGCCAGTTCATCGTTATAAGGTGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGc                                                                                  <  2:154520/143‑1 (MQ=255)
                                                                  gctgcAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGCTAACGCGTCAGAGTTTTATTAACTGATACAAATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATACCCGCCATAGTaa                                                                          >  1:464195/1‑143 (MQ=255)
                                                                  gctgcAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTAAGAGTTTTATTACCTAATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTaa                                                                          >  1:467170/1‑143 (MQ=255)
                                                                     gccaTCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACgg                                                                       <  1:15179/140‑1 (MQ=255)
                                                                              ttGCCAGTTCATCGTTATAAAGAGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATAAAGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCAAAAGTAACAATTATCAcaa                                                              >  2:103415/1‑141 (MQ=255)
                                                                               tGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCAAGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACctg                                                             <  1:23246/143‑1 (MQ=255)
                                                                                 ccAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAGGGCGTCCGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATGTCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACTGTTATACCctgct                                                           >  1:105655/1‑143 (MQ=255)
                                                                                 ccAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATCACctgcg                                                           >  1:363093/1‑142 (MQ=255)
                                                                                 ccAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACctgct                                                           >  1:247467/1‑143 (MQ=255)
                                                                                   aGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACGTGCTGt                                                         >  2:127428/1‑143 (MQ=255)
                                                                                                aaGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGtt                                            >  1:321636/1‑143 (MQ=255)
                                                                                                  ggCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTCCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTg                                          <  1:94387/143‑1 (MQ=255)
                                                                                                      ttttCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTa                                      >  1:239084/1‑143 (MQ=255)
                                                                                                      ttttCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTa                                      <  1:160775/143‑1 (MQ=255)
                                                                                                            aaTCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCaaaa                                >  2:290529/1‑143 (MQ=255)
                                                                                                             aTCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCATCATTGCTTTCACCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCaaaaa                               >  1:251368/1‑143 (MQ=255)
                                                                                                             aTCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCaaaaa                               <  1:367869/143‑1 (MQ=255)
                                                                                                              tCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCCTAGTAACGGTTATAACCTGCTCTAATCCTTGAGGTATGCTTAGCAAAAat                              >  2:366681/1‑143 (MQ=255)
                                                                                                               ccTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCCTAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAata                             >  2:103032/1‑143 (MQ=255)
                                                                                                                cTGATTACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAatat                            <  2:429972/143‑1 (MQ=255)
                                                                                                                  gATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATcc                          <  2:369907/143‑1 (MQ=255)
                                                                                                                       cgcgTTAGTGTTTTATTACCTGATACCGTTTCTGTCTAGCTATTGCGGATATCACGTAAGCCTATATGAAGAATATTCTCGCCATAGTAACGGTTATAACCTGCTGTACTAATTGAGGTTTGATTAGCAAAAATATCCCATTc                     <  2:267940/143‑1 (MQ=255)
                                                                                                                          gTCAGAGTTTTATTACCTGACACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGt                  <  2:436719/143‑1 (MQ=255)
                                                                                                                          gTCAGAGTTTTATTACCTGACACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGt                  <  2:52212/143‑1 (MQ=255)
                                                                                                                                      gtaCCTGATACCGAGTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCgg      <  1:489059/142‑1 (MQ=255)
                                                                                                                                          cTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCggggcg  >  1:305772/1‑141 (MQ=255)
                                                                                                                                          cTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGgag  >  1:141566/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                          
TGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAG  >  NC_000913/1575891‑1576171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑