| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,708,718 | G→A | 25.2% | A600A (GCG→GCA) | rsxC → | SoxR [2Fe‑2S] reducing system protein RsxC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,708,718 | 0 | G | A | 25.2% | 25.6 / 4.7 | 23 | A600A (GCG→GCA) | rsxC | SoxR [2Fe‑2S] reducing system protein RsxC |
| Reads supporting (aligned to +/- strand): ref base G (5/12); new base A (2/4); total (7/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
ATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAG > NC_000913/1708576‑1708859 | aTCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcg > 1:4718/1‑143 (MQ=255) tCCACGTACAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcgc < 1:42491/143‑1 (MQ=255) tCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGCCGCAGCCGCTATTGCCCGTGCCAAAgcgc > 1:468992/1‑143 (MQ=255) gcGTAAAACTGCCGCTGACGCAGCTATCGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCa < 2:488043/142‑1 (MQ=11) cctAAACCTGCCCTTCAAGCAGCTCTCGCCCGCGCCAACGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCaa < 1:136302/141‑1 (MQ=17) cGTAAAACTGCCGTTGAAGCAGCTATCGCGCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGAGCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCaa < 1:414566/143‑1 (MQ=255) aaaaCTGCCGTGGAAGCAGCTATCGCCCGCGCCAAAACGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCCATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCt < 1:169203/143‑1 (MQ=255) taaCCGCCGGTGAGGCAGCTGTCGCCCGCGCCAGAGCGCGCATGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTg < 2:318506/142‑1 (MQ=14) cTGCGGTCGAAGCAGCTAACGCCCGCGCAAAAGCGCGTGAGCTGGAACAACAACAGGCAAATGCGGAACCAGAAGAACAGGTCGATCCACGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa < 2:530583/143‑1 (MQ=11) cGTTGAAGCAGCTATCGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCACAGCGGCCGTCGAGGCCGCTATTGCACGAGCCAAAGCACGCAAGCTGGAACCGc > 2:457633/1‑143 (MQ=14) tATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAGGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGACCAGCCACAGGCGAATg > 2:343868/1‑143 (MQ=11) tATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATg < 1:526028/143‑1 (MQ=18) ccggcgcCACGGCGCGCAACCCGGAACAGCGCCAGGCAAATGCGGAACCAGCACACCTGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGaa < 2:458093/140‑1 (MQ=14) cccGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGaa < 1:169992/143‑1 (MQ=40) cagcaGCGCGCAAACTGGAACAGCCACAGCCAACTGCCGACCCAGCACCCCTGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCagaa < 2:337418/139‑1 (MQ=14) gCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCTGAACCAGGAGAACCGGTCGAta > 1:289250/1‑142 (MQ=255) cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCGAAGCGCGCAAGCAGGAACAGCAACGGGCTAATGCGGAGCCAGAAGAACAGGTCGAACCCCGCGAAgc > 2:525361/1‑143 (MQ=11) cTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCAAACGCCGCACCAGAGGAACAGGGCGATCCGCGCAGAGcggccggc > 2:229885/1‑141 (MQ=11) ccAGAACACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAt < 1:238890/143‑1 (MQ=255) ccgccgTCCAAGCCGCTATTGCCCGTGCCAAAGCGCGCACGCTGGTACGTCAACAGCCTAATGAGGATCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa < 1:229080/143‑1 (MQ=14) cgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAAc > 1:60888/1‑143 (MQ=255) cGCTATTGCCCGTGTCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCGGCTATTGCCCGCGCCCAAGCACGAAAAGTGGCACAGCAACAGGcga > 2:105204/1‑141 (MQ=14) ccGTGCCAAAGCGGGCACGCTGGAACAGCAACCTGCTGATGCGGCGCCACAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcggtac < 2:60888/143‑6 (MQ=11) ccAAGGCGCGCAAGCGGGAACAGCAGCAGGCGAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa < 2:18090/143‑1 (MQ=255) cAAAGGACGCAAGCCGCAAAAGCAAGAGGCTAACGAGGCAACAGAAGAACGGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 1:221241/143‑1 (MQ=14) cAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 1:323044/143‑1 (MQ=255) cgcgcAAGGTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAg < 1:343868/143‑1 (MQ=255) | ATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAG > NC_000913/1708576‑1708859 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |