| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,795,229 | A→T | 18.6% | intergenic (‑77/+24) | btuC ← / ← ihfA | vitamin B12 ABC transporter membrane subunit/integration host factor subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,795,229 | 0 | A | T | 18.6% | 51.6 / 6.6 | 28 | intergenic (‑77/+24) | btuC/ihfA | vitamin B12 ABC transporter membrane subunit/integration host factor subunit alpha |
| Reads supporting (aligned to +/- strand): ref base A (11/10); new base T (3/2); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01 | |||||||||||
AACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTA > NC_000913/1795090‑1795364 | accTGCCAGGCATAATAACCAGCGAATCTTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCg < 1:113419/141‑1 (MQ=255) tGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGcc > 1:154650/1‑143 (MQ=255) aCAGGCATAATAACCAGCGAATATTGTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCtt < 1:457377/143‑1 (MQ=255) cAGGCATAACAACCGGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCAGGGTATCCGTTCGGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCttt < 1:165876/143‑1 (MQ=255) gggCATAGGAACCAGTGAAGTGTTGGTCGCTGTTGTTGGCGGGCACGTGTCAGCATGTTATCCGTTCTGCTGAAGTGTCAGGGCGTTGATTTTCCGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCTCTGCGGCCtttt < 2:466194/142‑1 (MQ=255) aagctgcAGCGGATAGTTTGTCGCGGTTGTTGGCGGGCAAGTGTCAGCAGGGTATCCGTTCTGCTGAAGTGTCAGGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTttagtta < 1:96477/137‑1 (MQ=255) cgatatTTTCTTGTTGTTGTTGGCAGTGAAGTGTCAGTATGTTATTCGTTCTGCTGTAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGATAGTTAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTa < 2:279383/141‑1 (MQ=255) tatTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTc > 2:73478/1‑143 (MQ=255) gatTTGGGCGCTGGTGGTGGGGGGCAAGTGTCAGCATGGGAGCGGTGCTGCTGAAGTGTGATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGTGCGGCCTTTTTAGTTAGATCAGATTACTc < 2:132087/142‑1 (MQ=255) cgggggtgttgGCGGGCAAGTGTCAGCATGGCATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTgg < 2:461637/137‑1 (MQ=255) cGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGtttt < 1:279798/143‑1 (MQ=255) gggCACGTGTCAGCCTGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAACAGAACAAAGGCCGCAGAGCGGCCTTTTTCGTTCGCTCCGATTACTCCTCTTTGGGCGAAGCGttata > 2:253782/1‑140 (MQ=255) gggCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGGAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGTGCGAAGCGTTTTc > 1:439067/1‑143 (MQ=255) aaGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGAcc > 1:483717/1‑143 (MQ=255) tCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGACACGTTTTCTACCCGGCt > 2:500442/1‑143 (MQ=255) tCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCt > 2:310581/1‑143 (MQ=255) cGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAAcctattt > 1:367293/1‑137 (MQ=255) ggggcATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAg < 2:374833/139‑1 (MQ=255) tCATGGCGTTGATTTTACGGTGACTCTTCGACAGTAAAAAGAAAAAAGGACGCAGCGCGGCCTTTTACGTTAGATCAGATTACTAGTCATTGGGCGAAGCATTTTCGAACCGGATCTTTAAGTTCTGACCGGGCCTGAATGTc > 2:473214/1‑143 (MQ=255) gggCGTTGATTTGACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTcacc < 1:419564/142‑1 (MQ=255) tttACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAAAGCGGACTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGACGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGACGGTCACCACCCGACGTg > 2:165832/1‑143 (MQ=255) tctTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCAGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGa > 2:82867/1‑143 (MQ=255) taaaaaGGCCGCTCTGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCTTGCGGTAAGAGGAATAGCCTGGCCCGattg > 2:66315/2‑142 (MQ=255) taaaaaGGCCGCTCTGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGGCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCGCGCCCgcact > 2:456494/2‑139 (MQ=255) aaaaaGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGTGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTc > 1:420729/1‑143 (MQ=255) aaaGGCCGCTCTGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCGTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGGTTTCgg > 1:491182/1‑143 (MQ=255) ggCCGCAGAGCGTCCTTTTTAGTTAGATCAGATTACTCTTCTGTGGGCGAAGCGTGTTCGACCCGGCTTTTTGCCTTCTGCCCGTGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGtt < 2:413338/143‑1 (MQ=255) cccGCAGGGCGGCCTTTCGAGTTAGATCCGATTACTCGTCTCTGGGCGAAGCGTTTTCGACCCGGCTTGTTAACTACTGCCCGGGGCTGAAGGCCACCACGCGCCGGGCTGTAATGGGAATATTCTCGCCCGTTTTCGGGTTa < 2:232690/142‑1 (MQ=255) | AACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTA > NC_000913/1795090‑1795364 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |