| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,263,834 | G→A | 27.7% | intergenic (‑339/‑29) | fruB ← / → setB | fructose‑specific PTS multiphosphoryl transfer protein FruB/sugar exporter SetB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,263,834 | 0 | G | A | 27.7% | 36.8 / 15.3 | 30 | intergenic (‑339/‑29) | fruB/setB | fructose‑specific PTS multiphosphoryl transfer protein FruB/sugar exporter SetB |
| Reads supporting (aligned to +/- strand): ref base G (7/14); new base A (4/4); total (11/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
CGTTATCAGCAATTTCATTTCAGCAGCGTCAGCAGCCAATCAGCAACAGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCG > NC_000913/2263709‑2263970 | cGTTATCAGCAATCTTATTTCAGCAGCGTCAGCAGCCAATCAGCAACAGTGAAATGGCATTTTGACTATTCGTTGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGt < 2:30075/143‑1 (MQ=255) tATCAGCAATTTCATTTCAGCAGCGTCAGCAGCCAATCAGCAACAGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGAt < 1:172243/143‑1 (MQ=255) tATCAGCAATTTCATTTCAGCAGCGGCAGCAGCCAAGCAGCAACAGTGAAATGGCATGTTGACTATTCGTGGCGCTGTTGAGTTCGCTGGCTGTTTATTTTCTGACAAGCAGCGTG‑AACTCCGCGTCTTCCTCTTCCAGTGAt < 2:26024/143‑1 (MQ=255) aaTTTCATTTCAGCAGCGTCAGCAGCCAATCAGCAACAGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAg < 2:429913/143‑1 (MQ=255) gttttatttcaGCAGCGTCAGCAGCCAATCAGCAACAGTGGAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAgc < 2:517668/142‑1 (MQ=255) tttcattttaGTAGCGTAAGCAGCCAATGAGCAACAGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGTAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAgca < 1:23683/143‑1 (MQ=255) tttcatttcaGCAGCGTCAGCAGCCAATCAGCAACAGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAgca > 1:497360/1‑143 (MQ=255) tttcaGCAGCGTCAGCAGCCAATCAGCAACAGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAgcatgcat < 1:309818/143‑1 (MQ=255) tttcaGCAGCGGGAGGAGCGAAGCGGCAACAGTGGAATGGCATGTTGACGATTCGTGGCGCTTTTGATTTCGCTGTCGGTTTATTTTCTGACAAGCAGCGTACCAATGC‑CTTCTTCCTCTTCCAGTGATCGTCCAgcatgcat < 2:118254/143‑1 (MQ=255) agcagcCAATCAGCAACAGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCACGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAg > 1:392549/1‑143 (MQ=255) agcagcCAATCAGCAACAGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCACGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAg > 1:390781/1‑143 (MQ=255) agcagcCAATCAGCAACAGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCACGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAg > 1:390778/1‑143 (MQ=255) agcagcCAATCAGCAACAGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCACGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAg > 1:390774/1‑143 (MQ=255) ccAATCAGCAACAGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTcc > 1:195529/1‑143 (MQ=255) tCAGCAACAGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAgcg < 1:531167/143‑1 (MQ=255) tCAGCAACAGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAgcg < 2:289251/143‑1 (MQ=255) aGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCg < 1:339100/143‑1 (MQ=255) gggAAGTGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGt < 2:371596/141‑1 (MQ=255) tGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGGCTGCGGCGCGAACTAGtt > 1:72248/1‑143 (MQ=255) tGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCCCAGTCCCCCGCCCGCAATCGttccacc > 2:535632/1‑138 (MQ=255) tGACGATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCg < 1:518815/143‑1 (MQ=255) aCTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCACGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGAc < 2:390774/143‑1 (MQ=255) aCTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCACGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGAc < 2:390778/143‑1 (MQ=255) aCTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCACGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGAc < 2:390781/143‑1 (MQ=255) aCTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCACGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGAc < 2:392549/143‑1 (MQ=255) gcTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAAt < 1:239602/143‑1 (MQ=255) ttGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACATGACCTCGACGGCGTGTTTAATCGtt > 1:500653/1‑143 (MQ=255) tgtctgtTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCa < 2:497360/143‑1 (MQ=255) cTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTCTCTCACCGATATGGCGGgc > 2:2235/1‑143 (MQ=255) aaCTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTCTTAATCGTTGCCTCTCTCACCGGTATGACGGGCGCTCCGCCAACCCCg > 1:471089/1‑143 (MQ=255) | CGTTATCAGCAATTTCATTTCAGCAGCGTCAGCAGCCAATCAGCAACAGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTA‑AACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCG > NC_000913/2263709‑2263970 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |