| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,368,101 | T→C | 100% | A21A (GCT→GCC) | arnA → | fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,368,101 | 0 | T | C | 98.4% | 59.5 / ‑6.8 | 25 | A21A (GCT→GCC) | arnA | fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (13/10); minor base G (1/1); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGC‑AAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTG > NC_000913/2367968‑2368236 | acCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTCTGCGTCAATCCAGC‑AAGGAACATGCATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATCCCCCGGTACTGATGCCTCGCCGGCCGCCGGCTAc > 2:298888/1‑143 (MQ=255) caTGTCCGCGCCCCCCCCCGCTATTTGGTTCGGCAAGTTATCCGTCCATCCAGC‑AAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGaa < 2:368809/142‑1 (MQ=255) cgTCCGCGCCCACCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGC‑AAGCAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAAt < 2:83076/142‑1 (MQ=255) cgcgcCCGCTAGTGTGTTCAGCAAGTTATCCGTTCATCCAGC‑AAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTCCCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCAtt < 2:436893/143‑1 (MQ=255) cgcccCCGCTATTTTGTTGAGGACATCGTTCCCCCATCCTGC‑ATGGGTGGTGAATATTGAAAATCGTTGTTTTTGTCTATCTCGTTATGGGATGCGTCGGTATGGAAGCCCTGCGGGCCGCCGGTTACGAAATTAGCGCCAtt < 1:487860/143‑1 (MQ=255) cgcccCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGC‑AAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCAtt < 2:541612/143‑1 (MQ=255) cGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGC‑AAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTAcc > 2:252897/1‑143 (MQ=255) gttgttttCATCAAGTTACGCGTCC‑TTCAGCGCAGGAAAAGGAATAATGAAAGCTGTCGTTTTTGCTTACCACGATACGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACTAAATTAGCGCCATTTTTACCCATa < 2:414871/137‑1 (MQ=255) ttttGTTCAGCAAGTTATCCGTCCATCCAGC‑AAGGAAAATGAATACTGAAAACCATCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTGACGACATTACCGCCATTCTTACTCATAc > 2:18808/1‑143 (MQ=255) cAAGTTATCCGTCCATCCAGC‑AAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTCCGTATTTAGCGCCATTATTACCCATAATGATAATacc > 1:430230/1‑143 (MQ=255) cAAGTTATCCGTCCATCCAGC‑AAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGAACTGGGATAGCTCGGTATTGAAGACCTGCTGGCGGCCGATTCGGAGATTAGCGCCATTTTTGCCCATCATGATattgca > 2:57520/1‑138 (MQ=255) caGC‑AAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGACGGTTACGAAATTAGTGGTGTGACAACCCATAATCAAAATCACGGTGAAAAAGACTTCTa > 2:529650/1‑143 (MQ=255) aaaTGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGt < 1:376516/143‑1 (MQ=255) aaaTGAACAATGAAAACCGTCGTTTTTCCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGTTCTCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGt < 1:105277/143‑1 (MQ=255) gtcgtTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTCTTATGGTTCGAAAGTTCTTCCGGCGGCGGa > 2:353623/1‑143 (MQ=255) cgtTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGTGCCATTTTTACCTATTCTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGaaa < 1:303039/143‑1 (MQ=255) ttttGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAagag > 1:492362/1‑143 (MQ=255) cTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTc > 1:256301/1‑143 (MQ=255) aCGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGtt > 1:371906/1‑143 (MQ=255) atatGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGACAGAGGCATTCCGGTTTAt > 1:357600/1‑143 (MQ=255) tCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATTCTTTTCTTTTTCATAAAAATTTTTTTAATCAATCAATGACACGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAAc < 1:252897/143‑1 (MQ=255) gCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACACATACTGATAATCCCGCTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGACAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATcc > 2:269945/1‑143 (MQ=255) cccTGCTGGCGGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCTGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCAGTCCGGTTTATGCGCCGGATAACGTTAATCATCCg < 1:225664/143‑1 (MQ=255) ctgctgGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCt > 2:308702/1‑143 (MQ=255) tgctgGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCtg > 1:250009/1‑143 (MQ=255) | ACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGC‑AAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTG > NC_000913/2367968‑2368236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |