| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,661,821 | C→G | 100% | C104S (TGC→TCC) | iscR ← | DNA‑binding transcriptional dual regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,661,821 | 0 | C | G | 98.1% | 88.7 / ‑6.1 | 35 | C104S (TGC→TCC) | iscR | DNA‑binding transcriptional dual regulator IscR |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (14/19); minor base T (0/1); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTC > NC_000913/2661682‑2661953 | ggTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAAAGAGGTTGAGAAAACCGGTGAGGCGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGAtt > 1:74564/1‑143 (MQ=255) gTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGCGCGACGGTCGCTCAAATCCCGCCACGGCGCGTAGGTCAGGAATATATcg > 1:136543/1‑143 (MQ=255) tGGCCCGTCGTGAGTATGCTGACGACCAGCCACACCCAGCACTTCACGGTTATTAGCCTGTTAGCCGCACTGAAAACAGTTGGTAAAACCAGATAGACGGTCGCTCAAATCTCGCCACAGCGCGTGGGTCAGGGATTTATcgc < 2:532667/143‑1 (MQ=255) gcgcGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCCAATCACGCCCCCGCGCGTGGGTCACGGATTTATcgccg > 2:250302/1‑143 (MQ=255) tGAGTATGCTGACGACCAGACACATCCCGCACTTCCTGGTTATTACCCAGTTCGACAAAAGTAATGTTGTTGACAAAACCGGTGCGACGGTCGCTCAAATCACGCCACAGCGCGTGCGCCAGGGATTTATCGCCGCCCTGGcc > 1:178112/1‑142 (MQ=255) tGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGGCGCTCAAATCACGCCACAGCGCGTGGGGCAGGGATTTATCGCCGCGCTGGCa > 1:530237/1‑143 (MQ=255) aGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAgc > 1:285612/1‑143 (MQ=255) tATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAgccg > 2:50777/1‑143 (MQ=255) tATGCCGACGACCAGCCACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAgccg < 2:377212/143‑1 (MQ=255) cTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATATTGTTGAGAAAACCGGTGAGACGGTCCCTCAAATCACGCCACAGCGCGCGGGTCAGGGATTTACCGCCCCCCTGGCAGCCGCCtc > 2:360567/1‑142 (MQ=255) gacgacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATGTATCGCCGCCCTGGCAGCCGCCTTTa > 1:163996/1‑143 (MQ=255) gacgacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCATAATGTTATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTa < 1:250209/143‑1 (MQ=255) gacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGCTCTCTCGCCGCCCTGGCAGCCGCCCTTAccc > 1:160729/1‑143 (MQ=255) gacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAccc > 1:476366/1‑143 (MQ=255) ccAGACACATCCAGCACGTCCTAGTTATTAACCAGTTCGCCGAAAGTAATGTTGTTGAGAAAGCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTg < 1:214302/143‑1 (MQ=255) aTCCAGCACTTCGTGGTGATTAACCAGTGCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACggg < 1:120857/143‑1 (MQ=255) aTCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCCCAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCCGCCGCCTTTACCCTGACAACgcg > 1:190947/1‑141 (MQ=255) cAGCACTTCGTTGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTgg < 2:498155/143‑1 (MQ=255) aCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCGTCTAAAGATGCGTCCAc > 1:476880/1‑143 (MQ=255) cAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACgg < 2:102610/143‑1 (MQ=255) aactcgtgtggtCGAGAAGACCGTGGAGACGGTCGCTCAAATCACGCCCCCGCGCGTGGGTCACGGATTCATCGCCGCCTTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACt < 1:153669/137‑1 (MQ=255) tAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGAGCTTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACAGCTCGCCTCTACCGCTTCCTCCCCGGCGCTCATCACtccc > 2:458684/1‑140 (MQ=255) gaAATCCGGTGAGACGGTCGGTCAAATCACGCCGCAGCGCGTGGGTCAGGGATTTATCGCCGCGCTGGGAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAtg < 2:219689/143‑1 (MQ=255) aaCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAtgctg > 2:302027/1‑143 (MQ=255) aaCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAtgctg < 2:74564/143‑1 (MQ=255) ccGGTGACACGGCCGCGCAAATCCCGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTGTACCCTGGCAACGGGTGGCAGCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATgctgct < 1:219925/143‑1 (MQ=255) gagaCGCTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATCTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCAt < 1:457640/143‑1 (MQ=255) agaCGTTCGCTCATATTACGCCACAGCTCGTGTGTCATGGATTTATCGCCGCACTGGCAGCCGCGTTGACCCGGGCAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATc < 1:50770/143‑1 (MQ=255) agaCGTTCGCTCAAATCACGCCACAGCGCGTGGTTCTGTGATTTATCGCAGCACTGGCAGCCGCCTTTACCCGGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATc < 1:50777/143‑1 (MQ=255) gTCGCTCCAATCCCGGCACGGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGc < 1:105845/143‑1 (MQ=255) gTCGCTCACACCACGCCTCAGCGCAAAGGACACCTCTTCATCGCCGCCCTGGCAGCCGCCTTTACCCTCACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGc < 1:515385/143‑1 (MQ=255) cTCACATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTaa < 1:295547/143‑1 (MQ=255) taCGCCTCAGCGCGTGCGTCCGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGCCAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATaa < 1:289759/142‑1 (MQ=255) cgTGGGTCAGGGTTTTATCGCCCCCCTGTCAGCCGCCTTGACCTTGACAACGGGTGGTATCTACAGATTCGTCAACGGCGCTAATTCCTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGt < 2:463452/143‑1 (MQ=255) gTGGGTCAGGGATTTATCGCCGCCCTGGGAGCCGCGTTTTGCCCAATAACTGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTc < 2:530237/143‑1 (MQ=255) | GGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTC > NC_000913/2661682‑2661953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |