Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	674,768	G→T	100%	R6S (CGC→AGC)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,768	0	G	T	100.0%	76.5 / NA	28	R6S (CGC→AGC)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/13);  total (15/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAG‑CTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAA  >  NC_000913/674634‑674905                                                                                                                                        |                                                                                                                                          cATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAG‑CTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGct                                                                                                                                   >  2:496432/1‑143 (MQ=255)       tAGTCGACCAGAAGGATAGGGAAGCATAGACATGCGGGGATACTTCTCTTTGCTCTCGTCTTCGGTTACTGCAAATGTGCGCTTCTCATCCCAATAAAG‑ATGTACTTTGGCTTCTATCTCTTCCGGGCTGTATTGCTCTTGcg                                                                                                                             >  2:193976/1‑142 (MQ=255)       tAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCTAATGGGCGCAAAT‑AACTCAAGCATGTCTGTACTTTGGATTCTATCTCTTCCGCGCTGTATTGCTATTGCa                                                                                                                             >  2:532213/1‑143 (MQ=255)       tAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAATTGTGCGCTTCTCATCCCAATGCAG‑CTGTACTCTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGcg                                                                                                                             >  2:261228/1‑142 (MQ=255)       tAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAG‑CCGAAGTATAGATTCTATCTCTTACGGGCTGTATTGCCCTCGcc                                                                                                                             >  2:239368/1‑142 (MQ=255)             aCCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAG‑CTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAg                                                                                                                       >  1:193812/1‑143 (MQ=255)                   aGGATAGGTAAGCATAGACAGGCAGTACTCCTCCACTTTGTTCGCGTCTGCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAG‑CTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTg                                                                                                                 <  2:357052/143‑1 (MQ=255)                                             actACTTCTCTTTGCCCTCGTCTTCTGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAT‑CTGTACTTTGGATTCTATCTCTTCCGTGCTGTATTGCTCTTGCATGGCAGCCATTGGTCCTGTTTTCAATACGGCTACAAAt                                                                                       <  2:338527/141‑1 (MQ=255)                                                       ttttCTCTCGTCTTCGGTTACGTCAAATGTGCGCTTCTCATCCCAATGAAG‑CTGTTCTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGa                                                                             <  2:182454/143‑1 (MQ=255)                                                       tttGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAG‑CTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGGGGTCCTGTTTTCAATACGGCCAAAAATGTAGGGCTGa                                                                             >  2:281199/1‑143 (MQ=255)                                                                 tCTGCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAG‑CTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGttttt                                                                   <  2:288427/143‑1 (MQ=255)                                                                  cTTCGGTTCCTTCTAACGTGCGCTTCTCCTCCCGATGTAG‑CTGTACTTTGGATTCTATCTCTTCCGGGCTGGATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTc                                                                  <  2:352893/143‑1 (MQ=255)                                                                     cGGTTACTTCAAATGTGCGCTTCTAATCCCAATGAAG‑CTGTACTTTGGATTCTATCTCTTCCGGGCTCTATTGCTCTTGCATGGCAGCCAGCGGACCTGTTTCCAATACGGATACAAATGTGACGTTGAGATGATGTTACAGa                                                               >  2:289345/1‑143 (MQ=255)                                                                            ttCAAATGTGCGCTTCTCATCCCAATGAAG‑CTGTACTTTGGCTTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGCGGTCCTGTTTTCAATACGGCTACAAATGTAGCGCTGAGGTCGTTTTTCAGCTCCccat                                                        >  1:430679/1‑143 (MQ=255)                                                                            ttCAAATGTGCGCTTCTCATCCCAATGAAG‑CTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCgcat                                                        <  1:368685/143‑1 (MQ=255)                                                                            ttCAAATGTGCGCTTCTCATCCCAATGAAG‑CTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCgcat                                                        >  1:40368/1‑143 (MQ=255)                                                                             tCAAATGTGCGCTTCTCATCCCAATGAAG‑CTGTACTTTGGATTCTATCGCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACACATGTAGCGTTGAGGTGGTTTTTCAGATCCgcata                                                       <  2:494191/143‑1 (MQ=255)                                                                                agtgtgGGCTTGTCAGCGCAATGAAG‑CTGTACTTTGGGTTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCGAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCgcatagca                                                    <  2:46748/141‑1 (MQ=255)                                                                                      cgcgTCTCATCCCAATGAAG‑CTGTACTGTGGATTCTATCTCTTCCGGGCTGTATTGCTCGGGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGccc                                              <  2:62608/143‑1 (MQ=255)                                                                                               tCCCATTGAAG‑CGCTACTTTGGATTCTCTCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTGTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCcg                                     <  1:535819/143‑1 (MQ=255)                                                                                                           cTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATCCACGACATTCCTCCTGTTTTCAATACGGCTACAAATGAACCGCCAAGGCGTTTTTTCAGACCCGCATCGCATGCCCCGAGCGTCCGCGACTCAACAg                          >  2:503381/1‑143 (MQ=255)                                                                                                                 tttGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGGTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCCAACGTCCGCGTCCAAACAGCCTTTc                    >  2:44798/1‑143 (MQ=255)                                                                                                                   ttgATTCTTGCTATTCCGTGTTGTATGGCTCTTGGATGGCAGGCGGTGTTCCTGTTTTCAATACGGCTACAATGGTAGCGTTGAGGTGGTTTTTCAGATCTGCATAGCATAGCTCAAGCGTCCGCGTCAAAACAGCGTTTcgc                  <  2:337067/141‑1 (MQ=255)                                                                                                                              tctTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTGTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTGTGGGGCACTGGACgg       >  1:155632/1‑142 (MQ=255)                                                                                                                                ttCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAACTGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCGAACGTCCGCGTCAAAACAGCCTTTCGTGCACTCGACGGTg     >  1:98058/1‑143 (MQ=255)                                                                                                                                  ccGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGGTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGACAAAACAGCCTTTCGAGCACTCGACGGTGaa   >  2:457557/1‑143 (MQ=255)                                                                                                                                   cGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGaaa  <  1:454208/143‑1 (MQ=255)                                                                                                                                   cGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGaaa  <  1:454481/143‑1 (MQ=255)                                                                                                                                        |                                                                                                                                          CATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAG‑CTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAA  >  NC_000913/674634‑674905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑