Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,657,599 T→A 10.6% intergenic (+32/+387) hdeD → / ← arrS acid‑resistance membrane protein/small regulatory RNA ArrS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,657,5990TA10.6% 109.1 / 2.3 43intergenic (+32/+387)hdeD/arrSacid‑resistance membrane protein/small regulatory RNA ArrS
Reads supporting (aligned to +/- strand):  ref base T (19/18);  new base A (2/3);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATT  >  NC_000913/3657465‑3657741
                                                                                                                                      |                                                                                                                                              
gATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGATCTGATATTTAGCGCCGCCAACCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGa                                                                                                                                        <  1:288126/143‑1 (MQ=255)
gATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAACATAGTCACTTGATGTGa                                                                                                                                        >  2:381266/1‑143 (MQ=255)
   ggTGTCTGTAACGCTGGGTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTa                                                                                                                                     <  1:205083/143‑1 (MQ=255)
    gtgtCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCGCCGGGAGCATAGTCAGTTGAGGCGACTAt                                                                                                                                    >  1:199272/1‑143 (MQ=255)
    gtgtCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTAt                                                                                                                                    <  1:514844/143‑1 (MQ=255)
       tcggTAGCGGTGGTTTCCACACGGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGAt                                                                                                                                 <  1:206724/140‑1 (MQ=255)
           tAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATGATTACGCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGa                                                                                                                             >  1:175446/1‑143 (MQ=255)
            aaCGCTGGTTTCCACACTGGTGGGAATTGAACTGGTGTTTAGCGCCGCCAGCCTGTTCAGCTTCGGCAGTTTGTGCGGTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGAt                                                                                                                            <  2:359176/143‑1 (MQ=255)
                       gcacgcgggtggGAATTGAACTGGTGTTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATActctct                                                                                                                 <  2:214678/136‑1 (MQ=255)
                               gtggGGACTGAGCTGATATTTAGCGCCGCCAGCCGGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACg                                                                                                         <  2:437208/143‑1 (MQ=255)
                                  ggAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCCGTTGTCACCCGGACCATAGCCACATGATGTCCCTATGATCCCCTTAATACCCCCTCCACTACCCac                                                                                                      >  2:539683/1‑142 (MQ=255)
                                    aaTTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGGGCATAGTCACTTGAGGTGACTATGATACGAGTACTACTCTCTCCGCTACGCAgtg                                                                                                    >  2:387066/1‑143 (MQ=255)
                                      ttGACCTGATATTTAGCGCCGCCAGCCTGGTCAGCTTCGCCAGTTTGTTCGTTAAGCACCAATAAGCACCCCGGCTGTCACCCGGACCATATTTACTTCATGCGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgtt                                                                                                  <  1:92500/143‑1 (MQ=255)
                                      ttGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgtt                                                                                                  >  2:436416/1‑143 (MQ=255)
                                        gcaCTGATAGTTAGCGCCCCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgttgt                                                                                                <  1:268178/141‑1 (MQ=255)
                                         aaCTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTa                                                                                               <  1:58503/143‑1 (MQ=255)
                                            ttttatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGAt                                                                                            <  2:153508/140‑1 (MQ=255)
                                            tGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGAt                                                                                            <  1:546989/143‑1 (MQ=255)
                                            tGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGAt                                                                                            >  2:513793/1‑143 (MQ=255)
                                                atTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGGCACTTGATGTGCCTATGATCCGATTACTCCTCTCTCCGCTACGCAGGGTTGTCGATCaag                                                                                        >  2:538653/1‑142 (MQ=255)
                                                   tAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACCACGTCTTGTAGACCACTTgc                                                                                     >  2:32095/1‑143 (MQ=255)
                                                      cgccgcCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATCTTCTTTCCGCTACGCAGTGTTGTAGATCAATTGCGCa                                                                                  >  1:369829/1‑143 (MQ=255)
                                                       gccgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCTCCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCAc                                                                                 >  2:368727/1‑143 (MQ=255)
                                                       gccgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTAAAATGAGGTTACTATGGTTCGATTGATCCTCTCTCCGCTTCGCCGTGTTGTAACTCATTTgcgccc                                                                                 >  1:488070/1‑141 (MQ=255)
                                                             aGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGGTCAATTGCGCACTATCAt                                                                           >  2:339072/1‑143 (MQ=255)
                                                              gCCTGTTCGGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCact                                                                          <  1:462275/143‑3 (MQ=255)
                                                              gCCTGTTCCGCTTCGCCGGTTTGTGCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCAtt                                                                          <  2:405812/143‑1 (MQ=255)
                                                                      aGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAaataat                                                                  <  1:446848/143‑1 (MQ=255)
                                                                        cTTCGCCAGTTTGTTCGTTGAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTGACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTa                                                                <  2:269264/143‑1 (MQ=255)
                                                                             ccATTTTGTTCGTTAATCAGCAATACTTTCCCCGGTTGTCACCCGGAGCATAGTCACGTGATGTGTCTATGATCCGATTACTACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGc                                                           <  1:30581/143‑1 (MQ=255)
                                                                                   ggTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCTGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGa                                                     <  2:414200/142‑1 (MQ=255)
                                                                                     ttCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTCTCATTGAAATAATTACCTGCTAGTGatt                                                   >  2:172475/1‑143 (MQ=255)
                                                                                     ttCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGatt                                                   >  2:298936/1‑143 (MQ=255)
                                                                                      tCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGGTCAATTGCGCACTATCATTGCAATAATTACCTGCTCGtatttt                                                  >  1:421339/1‑138 (MQ=255)
                                                                                       cGTTAAGCAGCAATAGTTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGattat                                                 <  2:280770/143‑1 (MQ=255)
                                                                                            agcagcAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCaa                                            <  2:70353/143‑1 (MQ=255)
                                                                                            agcagcAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGCGACTCTGATCCGATTACTACTCTCTGCCCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATCACATGCTAGTGATTCTTTGaa                                            >  2:477148/1‑143 (MQ=255)
                                                                                             gcagcaATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATAGTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAAc                                           >  2:273418/1‑143 (MQ=255)
                                                                                                 cgataatTGCGCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTAc                                       <  2:287173/141‑1 (MQ=255)
                                                                                                   ataatTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATACGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAACTCATTACCTCCTAGTCATTCTTTCAACCTACTg                                     >  1:486465/1‑143 (MQ=255)
                                                                                                             cGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCCTTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTCCATCt                           >  2:363417/1‑143 (MQ=255)
                                                                                                                          gATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCAct              >  2:405520/1‑143 (MQ=255)
                                                                                                                             cATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTa           <  2:369829/143‑1 (MQ=255)
                                                                                                                                      ttGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAAtt  <  2:546717/143‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                              
GATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATT  >  NC_000913/3657465‑3657741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑