Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5430CA100.0% 124.8 / NA 40P1100Q (CCG→CAG) rpoBRNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/21);  total (19/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCA  >  NC_000913/4184413‑4184677
                                                                                                                                   |                                                                                                                                      
aaaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAAATAGGGCGTAATGCCGACCAACCAATTTTAATG‑ACATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAcc                                                                                                                            >  1:401292/1‑143 (MQ=255)
     ccggacccAGCCGGGTGACAAGATGGTAGGTCGTCACGGTAACGAGGGTGTAATTTCTA‑AGATCTACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc                                                                                                                       <  2:442153/137‑1 (MQ=255)
     ccGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGACCCAGCTGGGCGTACCGtctc                                                                                                                       >  2:340273/1‑143 (MQ=255)
         aTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGATATTCTAGGTTAGTTTGTACTGCACCATCTGCGCGTACCGTCTCGTAt                                                                                                                   >  1:396216/1‑143 (MQ=255)
          tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                  <  2:33896/143‑1 (MQ=255)
          acaaggcTGGTGACAAGATCGCGGGTCGGCACGGTAACAAGGGTGTAAGTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGACAACGGTACGCCGGTAGACATCCTACTGAACCAGCCGGGCGTACCGTCTCGTATg                                                                                                                  <  2:543743/137‑1 (MQ=255)
           ccAGCCTGCTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGa                                                                                                                 <  1:311381/143‑1 (MQ=255)
               ccTGTTGACAAGATTGCAGGTCGTCTCGGTGACAAGCGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTGACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAt                                                                                                             <  1:67684/143‑1 (MQ=255)
                  ggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCgg                                                                                                          >  1:31822/1‑143 (MQ=255)
                      aCAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGGACTGAACCAGCTGGGCGTAGCGTGTCGTATGAACATCGGTGAg                                                                                                      >  1:216446/1‑143 (MQ=255)
                        aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt                                                                                                    >  1:108977/1‑143 (MQ=255)
                         agagGGCAGGCCGTCACGGTAACAGGGGTGTAATTTCCA‑AGACCACCCCCATCGAATATATGCCTTACGATGCAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATc                                                                                                   <  1:310897/143‑1 (MQ=255)
                         aGATCGCAGGTCGTCACGGTAACAAGGGTGGAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGATATCGTACTGAACCAGCTGCGCGTACCGTCTCGTATGAACATCGGTCAGATc                                                                                                   <  2:286315/143‑1 (MQ=255)
                           aTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAGCCGGTACGCAGATAGTCTTGGTATTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt                                                                                                 <  2:388806/143‑1 (MQ=255)
                                 ggTCGGCACGGTAACAAGGGTGTAATTTCTA‑AGAAGAGTGCGATCGAAGCTATGCCTTACGATGAAAACGGTACTCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAc                                                                                           <  2:112155/143‑1 (MQ=255)
                                     gtcACGGTAACAAGGGGTTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAATGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAc                                                                                       <  2:69243/143‑1 (MQ=255)
                                       cACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCt                                                                                     <  1:428759/143‑1 (MQ=255)
                                                aaGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGc                                                                            <  1:473048/143‑1 (MQ=255)
                                                  gggTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTg                                                                          >  1:272620/1‑143 (MQ=255)
                                                        aaTTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTGGTATGAACATCGGTCAGATGCTCGAAACCGACGGGGGGATGGCCGCGAAAg                                                                    >  1:29349/1‑143 (MQ=255)
                                                              tA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCg                                                              >  1:171815/1‑143 (MQ=255)
                                                                   aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGACACCCACCTGGGTATGGCTGCGAAAGGTATCGGCgc                                                          >  1:547716/1‑142 (MQ=255)
                                                                   aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCCGGGTGCGTCTGCGAATGGTATCGGCGa                                                          >  2:212758/1‑143 (MQ=255)
                                                                    tCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGAGTATGGCTGCGAAAGGTATCGGCGAc                                                         <  1:79193/143‑1 (MQ=255)
                                                                      aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                       >  1:102806/1‑143 (MQ=255)
                                                                        cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGACAGGTATCGGCGACACGa                                                     >  1:153200/1‑143 (MQ=255)
                                                                             tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                >  1:153802/1‑143 (MQ=255)
                                                                               gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGACTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTCTGGCTGCGAACGGTATCGGCGCCCAGAGCAACga                                              >  2:153085/1‑142 (MQ=255)
                                                                                aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACACACCTGGGTATGGCTGCGAAAGGTATCGGCGACGAGATCAACGcc                                             >  1:359957/1‑143 (MQ=255)
                                                                                aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGGCTCGTATGCACATCGGTCAGATCCGCGAAACCCACCTGGGAATGGCTGCGAAAGGAATCGGAGACAAGACCCACGcc                                             >  2:55967/1‑143 (MQ=255)
                                                                                         cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTAAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                    <  2:445520/143‑1 (MQ=255)
                                                                                          ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaacc                                   >  2:400693/1‑141 (MQ=255)
                                                                                              gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                               <  2:31822/143‑1 (MQ=255)
                                                                                              gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                               <  1:169902/143‑1 (MQ=255)
                                                                                                  aaaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                           <  1:83775/143‑1 (MQ=255)
                                                                                                        gtctgCCGGTAGCCCTCGTACTGAACCAGCTTGCCGTACTGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGTTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                     <  2:225132/139‑1 (MQ=255)
                                                                                                                   gACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg          <  2:401292/143‑1 (MQ=255)
                                                                                                                       tggTACTGGACCAGCTGGGCCTACCGTCTCGTATGTACATCGCTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGaa      <  2:142984/141‑1 (MQ=255)
                                                                                                                       tcttACTGTACCAGCCAGGCGTACCGTCTCAAGTTTAGATCGGTCAGAGCCTCGAAACCCACATGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGaa      <  2:357104/140‑1 (MQ=255)
                                                                                                                           aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa  >  1:474748/1‑143 (MQ=255)
                                                                                                                                   |                                                                                                                                      
AAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCA  >  NC_000913/4184413‑4184677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑