Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,600,614 T→C 12.6% N121S (AAC→AGC)  dnaC ← DNA replication protein DnaC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,600,6140TC12.6% 62.2 / 2.9 32N121S (AAC→AGC) dnaCDNA replication protein DnaC
Reads supporting (aligned to +/- strand):  ref base T (11/17);  new base C (2/2);  total (13/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.09e-01

CAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAACGCATTCATCTGCCCTTCACACTCAACGCGAT  >  NC_000913/4600478‑4600755
                                                                                                                                        |                                                                                                                                             
cagcagATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCACGTTGCAGa                                                                                                                                         >  2:372649/1‑143 (MQ=255)
            gTTGCTCGGATCGTGACGCAGTGGTTCGTCGCTGGGACCGGTATTCCTGCAGGTATCTTCCATCGCCGACATAATATCGGCCACGGTGATGGTCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCa                                                                                                                             <  2:48739/143‑1 (MQ=255)
                        gTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTGATAGCCGACATAATATCGGCAACGGTGATGATCAATACGTGATTAGCGCGTAGCAGCAGATCGTTGCACATTACCGCGGCGAGATgggctgtcc                                                                                                                 >  2:53313/1‑136 (MQ=255)
                                tttgttCTTCGCTGGTACCGCTATTACTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCt                                                                                                         <  2:255771/142‑1 (MQ=255)
                                   gttCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGc                                                                                                      >  1:187700/1‑143 (MQ=255)
                                     gatctgCTGGTACCGCTATTTCTGAGGGGATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTACCTGGCtt                                                                                                    >  2:332800/6‑143 (MQ=255)
                                              gTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGCTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTCCCTGGATTACCAGacaa                                                                                           >  2:50379/1‑143 (MQ=255)
                                              gTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGCTGCAGATTGCCGCCGCCAGATGGTTTTTGCAGGTTCCTGGCTTACCAGaaaa                                                                                           >  2:50393/1‑143 (MQ=255)
                                                 ccGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGAt                                                                                        <  1:257719/143‑1 (MQ=255)
                                                     tATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGCTGAAg                                                                                    >  2:300681/1‑143 (MQ=255)
                                                            gAAGGTAGCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGa                                                                             <  1:486696/143‑1 (MQ=255)
                                                                 tATCTTTCATCGCCGACATAATATCGGCCACGGTGACGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTg                                                                        <  1:32121/143‑1 (MQ=255)
                                                                  aTCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGc                                                                       <  1:62519/143‑1 (MQ=255)
                                                                     tttCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGt                                                                    >  1:296701/1‑143 (MQ=255)
                                                                        cATCGCCGACATACGATCGGCCACGGTGATGCTCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCaa                                                                 <  1:431783/143‑1 (MQ=255)
                                                                        cATCGCCGACATAATATCGGCCACGGTGATGATCAATCCGGCTTGACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCaa                                                                 <  2:187700/143‑1 (MQ=255)
                                                                             ccGACATAATGTCGTCCACGGTGATGATCAATACGGCTTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATTAAGCTGGCGATGTTGCCGTCAAActct                                                            <  2:276194/143‑1 (MQ=255)
                                                                                  ataataTCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGAc                                                       >  1:264908/1‑143 (MQ=255)
                                                                                    natatCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATGGCTGCGTGCAGATGGTTTTTGCCGGTGCATTGCTTCGGAAGAAAGGTGGGGCTGGCGCGGGGGCGGTCAACCTCTTGGACag                                                     >  1:66849/2‑142 (MQ=255)
                                                                                                 gTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTAGTTGCAGATTGCAGCCGCCAGATGGTTTTTGAAGGTTCCTGGCTTACAAGAAAAGATGAAGCTGGCGATGTTGCCGTGAAAATCTTCGATATACTGgcgcgcgtg                                        >  2:398921/1‑140 (MQ=255)
                                                                                                  tgatgcTCAATACGGAGTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTCCAGTCAAACTCTTCGACATACTGGCGCGCTTTg                                       <  2:194068/143‑1 (MQ=255)
                                                                                                        ttagtgcGGATTTTACGCGTAGCATGGGGTCGTTGCAGCGGGCCGCCGACAGATGGTTTTTGCCTGTTCCTGGCTTACCAGAAAATATGAAGCTGGCGATGTTGTCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAAc                                 <  2:441177/137‑1 (MQ=255)
                                                                                                             aCGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGTCGTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAACGCAtt                            >  2:264735/1‑143 (MQ=255)
                                                                                                                   ttACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACAAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAACGCATTCATCTg                      >  2:180118/1‑143 (MQ=255)
                                                                                                                   gtACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCCGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAACGCATTCATCTg                      <  1:521563/142‑1 (MQ=255)
                                                                                                                            agcagcagcTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAACGCATTCATCTGCCCTTcaca             <  1:246534/143‑1 (MQ=255)
                                                                                                                             gcagcagcTCGCTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAGGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAACGCATTCATCTGCCCTTCacac            <  1:50393/143‑1 (MQ=255)
                                                                                                                             gcagcagcTCGCTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAGGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAACGCATTCATCTGCCCTTCacac            <  1:50379/143‑1 (MQ=255)
                                                                                                                              cagcagcTCGTTGCAGATTTCCGCCGCCAGATGGTTAATGACGGTTTCTGGCTTACCAGAAAAGATGAATCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAACGCATTCATCTGCCCTTCACACt           <  1:455394/143‑1 (MQ=255)
                                                                                                                               agcagcTCGTGGCAGATTTGCGCCGCCGGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAACGCATTCATCTGCCCTTCACACTc          <  2:48669/143‑1 (MQ=255)
                                                                                                                                      cGTTGCTGGTTGTCGCCGCCACATGGTTTTTGCCGGTTCCTTGCTGACCACAAAAGATGAAGCTTGCGATGTTGCCTTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAACGCATTCATCTGCCCTTCACACTCAACGCGa   <  2:441618/143‑1 (MQ=255)
                                                                                                                                       gTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAACGCATTCATCTGCCCTTCACACTCAACGCGAt  <  1:538614/143‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                             
CAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAACGCATTCATCTGCCCTTCACACTCAACGCGAT  >  NC_000913/4600478‑4600755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑