| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 696,470 | C→T | 100% | noncoding (35/75 nt) | glnX ← | tRNA‑Gln |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 696,470 | 0 | C | T | 100.0% | 49.2 / ‑6.7 | 21 | noncoding (35/75 nt) | glnX | tRNA‑Gln |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (11/9); total (12/9) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCG > NC_000913/696339‑696601 | cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTg > 1:389061/1‑143 (MQ=255) gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGc > 1:27534/1‑143 (MQ=255) aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCg > 2:191775/1‑143 (MQ=255) ggtAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCtt < 2:8574/141‑1 (MQ=255) tAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg > 1:432135/1‑143 (MQ=255) aaGACGCGTCAATATCTCATTCTTTATTGAATTAACTCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGc < 1:199508/143‑1 (MQ=21) aTCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTg > 1:225439/1‑143 (MQ=255) cactCGACATTGGATGAACGCAGAAAGGCGAAAAGTCCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGCGGCTTCGCACCTCGGAATGCGGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTg < 2:84417/140‑1 (MQ=21) gACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGAACGAGGATGCGAACATCGGAATGCCGGACACAGACTCCAGTACCTAACCGCTTGGCGCTAACCCAACAGGATggaagt > 2:136032/1‑138 (MQ=255) aTTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCCACTGGGTGCACTTACa > 2:318316/1‑143 (MQ=255) aTTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCACTTACa > 2:458856/1‑143 (MQ=255) gCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCGATTACAAGGTAAGCGTCTTGAATaa > 1:527648/1‑143 (MQ=255) cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATaaa < 2:432135/143‑1 (MQ=255) cagcctgtttAATGTGGCTGGGGTAGGAGGATTCGAGCCTCGGCATTCCGGAATTAGAATCCGGTGCCTTAGCGCTTGGCGATACCCGAACTGGGTGCACTTACAAGGTAAGTGTCTTGAATAAATTGGCTGGGGTACGAGGa < 2:34251/136‑1 (MQ=255) tgtttttttAATGTTGTTGTGGTGCGACGATTTGATCTTTGGTTTGGCGAACTTAGGATCCGATGACTTTCGGCTTGGCGTTTCCCTAACTGGGTGTCCTTTCTCGTTAAGCCTCTTGAATATATTGGCTGGGGTACGAGGAt < 2:267097/140‑1 (MQ=255) aaTGTGGCTGGGGTACGAGTATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTc < 2:335681/143‑1 (MQ=255) ggggTACGGGGCTTCGAACCACGGAATGCCGGAATTAGAACCCGGTGCCTTACCGCGTGGCGAACCTCCAACTACGTCCTACCACGGCAATATCGTCTTGAATAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCg < 2:115191/143‑1 (MQ=16) ggATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa < 1:419456/143‑1 (MQ=255) ggATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGATGCATTTGCATGGTCCGCGTCTTGAAGAAACTGGCAGGGGTACGAGGCTACGACCCGCGGAGAGCCGGAATCacca > 2:307387/1‑140 (MQ=17) gAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGAGTGCAATTACAAAGGAGGCAGCTCGAATATCTTGGATGCGGTGCGAGGCTTCGAACCGCGGAAGGCCAGAACCAGAATccaga > 2:322739/1‑140 (MQ=12) aaTGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGATGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCAGGAGTACGAGGAGACGAACAGCGGAATGGCGGAAACAGCATCCGGAGCCCAACCg > 2:50247/1‑143 (MQ=21) | CGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCG > NC_000913/696339‑696601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |