Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	760,485	G→A	100%	G594S (GGC→AGC)	sucA →	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	760,485	0	G	A	100.0%	40.7 / NA	15	G594S (GGC→AGC)	sucA	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/5);  total (10/5)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTACAC  >  NC_000913/760345‑760624                                                                                                                                             |                                                                                                                                            aCAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGc                                                                                                                                           >  2:77328/1‑143 (MQ=255)            acgacgcgcCCGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaaaa                                                                                                                                <  2:484187/138‑1 (MQ=255)                      tGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTGATGGCGATCGCCAGGCGATGGCTACCGGTGATAAACTGCTAGACTGGAGCGGTGCGGAAAACCTCGCTTAc                                                                                                                      >  2:133760/1‑143 (MQ=255)                                                 cGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAACCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCg                                                                                           <  1:102429/143‑1 (MQ=255)                                                           aGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGt                                                                                 >  1:364686/1‑143 (MQ=255)                                                                ttGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCGTTCCGGCTCGCCTGTCGGGt                                                                            >  1:143673/1‑143 (MQ=255)                                                                                cgTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTcgcg                                                            >  1:524004/1‑143 (MQ=255)                                                                                               tGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCCCGCTGTGTCATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAcc                                             <  1:77328/143‑1 (MQ=255)                                                                                                       gCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTGTTCCACCGCCAcgcg                                     >  1:64651/1‑143 (MQ=255)                                                                                                            gCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCCCGCGGTGAt                                >  1:428695/1‑143 (MQ=255)                                                                                                            gCGATGGCTGCCCGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGACGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGAt                                <  1:323168/143‑1 (MQ=255)                                                                                                                      caggTGGGAAGCGGATCGACTGGAGCGGTGGGGAAAACCGCGCTGACGCCACGCTGGTTGATGAAGGCATGCCGGTTCGCCTGTCGGGTGAAGACGCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAg                      <  2:397832/141‑1 (MQ=255)                                                                                                                              aaaCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGCTCCGCAACGAGTCTAACgg              >  1:491881/1‑143 (MQ=255)                                                                                                                                     tCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCAGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACAAGTCTAACCGTTCCACt       >  1:157268/1‑143 (MQ=255)                                                                                                                                          tGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCCGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTCCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGGTCGACTTacac  >  2:408863/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                            ACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTACAC  >  NC_000913/760345‑760624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑