| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 798,039 | C→T | 13.9% | L131L (CTG→CTA) | ybhA ← | pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 798,039 | 0 | C | T | 13.9% | 61.1 / 3.4 | 29 | L131L (CTG→CTA) | ybhA | pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (12/13); new base T (2/2); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01 | |||||||||||
ACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAA > NC_000913/797908‑798172 | aCATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCGGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGCAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTGTgcgc > 1:225093/1‑143 (MQ=255) ttACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTGACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCAGACGCTGTTCCGGAGGCAGGGGTTGCGCCCAggt > 2:156836/1‑141 (MQ=255) cAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAGCTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGCGTGAAAGGCCGACGCTGTTCCGGCGGCCCGGTTTGCGCCCAGCTCGAt > 2:515770/1‑143 (MQ=255) cAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGAt < 1:170465/143‑1 (MQ=255) cAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGAt > 1:544525/1‑143 (MQ=255) cactggCGGCAGGTCATCGGGCGTCAGGGCGAACGTGCATACGGCGTGAACTTGTTGCGCCGTTTCAGCCAGCGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGa < 2:35380/138‑1 (MQ=255) cAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGTAGGGTTTGCGCCCAGTTAGATGTGCGAATGa > 1:169313/1‑143 (MQ=255) cAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGTAGGGTTTGCGCCCAGTTAGATGTGCGAATGa > 1:337750/1‑143 (MQ=255) cAATTGCGGCAGGTCATCGTGCGGCAGGGCGACCTACCATACAGGTTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGa < 1:173146/143‑1 (MQ=255) aTTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACa < 1:501498/143‑1 (MQ=255) cATCGTGCGTCAGGGCGAACTTCCACTCGGCGTCAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCCGTTAGATGTGCGAATGACATGCCCGGTCggg < 1:149677/143‑1 (MQ=255) gTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGCCATGCCCGGTCGGGTGCTCATa > 1:230713/1‑143 (MQ=255) gattgtCCATGTGGCGTTAGCTTTGTGCGCCGTTTGAGATATGGATTCGGCGTTTGTGAAAGGCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTg < 2:240418/138‑1 (MQ=255) cTTCCATCCGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGcat < 1:61147/143‑1 (MQ=255) cTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGcat < 1:332751/143‑1 (MQ=255) cATCCGGCGTTAACTTGTTGCGCCGTGTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCatcatc < 2:342312/143‑1 (MQ=255) tACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCCTCgc > 1:277639/1‑142 (MQ=255) ttgttgCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGAc > 1:207813/1‑143 (MQ=255) gttgCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGTAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGACCg < 2:169313/143‑1 (MQ=255) gttgCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGTAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGACCg < 2:337750/143‑1 (MQ=255) gTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGACCGTGAAtgtg < 1:222702/143‑1 (MQ=255) gTTGCAGCCAGAGAGGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGACCGTGAAtgtg < 2:396478/143‑1 (MQ=255) aGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACCTGCCCGGTCCGGTGCTCATAGACCATTGCATCCTCGACCTCCATCAGCCCGTGAATGTGGTGtt > 1:209396/1‑143 (MQ=255) cAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATGGACATACCTGGGACCGTGGATGGGgcgtgaat > 2:152256/1‑139 (MQ=255) tgAAAGTCGGACGCTGTTCCCGCGGCAGGGTTAGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGt > 2:285466/1‑143 (MQ=255) gAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACCTACATCAGACCGTGAATGTGGTGCTCATTCAGCCTCTCAATGAgct > 2:345527/1‑141 (MQ=255) ggACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGACCGTGAATGTGGTGTTCATTCAGCATATAAAAAAGATGCAGGGc > 1:942/1‑143 (MQ=255) cagcggTTCCTGCGGCTGGGTTTCCCCCAACTTAGATGTGCGGATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCAGCGACATACATCAGACCGTGAATGTTGTGTTCATTCAGCTCCTCAATGAGTTGCAGGGCtt < 2:432294/138‑1 (MQ=255) ttCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTtaataa < 2:230713/143‑1 (MQ=255) | ACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAA > NC_000913/797908‑798172 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |