Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	pseudogene (151/758 nt)	glpR ←	DNA‑binding transcriptional repressor GlpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	83.4 / NA	29	pseudogene (151/758 nt)	glpR	DNA‑binding transcriptional repressor GlpR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (13/16);  total (13/16)

GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTT  >  NC_000913/3560316‑3560584                                                                                                                                             |                                                                                                                                  gATAAACAGCGTCGATCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGTTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgca                                                                                                                                 <  1:410265/143‑4 (MQ=255) gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGTGTGGCCTTGCGATCGTGCCACGGCATGTTAACCAAACTGGAAGGAAGCGCCGCACCTCCATGATGGcgca                                                                                                                                 >  1:406690/1‑140 (MQ=255)      gctgtgTCGAGGCGTGGGGGATTTGGTCGGCCACTTGGCGGGTGGTGCGCGCTTTTGCGTCGGTCTGTGTGGCGTTGCGATCGTTACACGGCGTGTTAACCGGACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATc                                                                                                                            <  2:853820/138‑1 (MQ=255)       cAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCa                                                                                                                           <  1:383803/143‑1 (MQ=255)            tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGttt                                                                                                                      >  1:42306/1‑143 (MQ=255)                 ccATTGGGTGTTTGCGCCGCCACTTTGCGGGCGATGCGCTCTTTTGCCGCGGTCCGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTc                                                                                                                 <  1:493006/143‑1 (MQ=255)                               cTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGa                                                                                                   <  1:674835/143‑1 (MQ=255)                                          tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTCAACAAACTGCAAGGAAGCGCCGCACCGACACGAAGGCGAAGGATAAGGTTCTGCTCCGCCAGCGCATCGAAGAcgcgcgga                                                                                        >  2:786426/1‑139 (MQ=255)                                                     cgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCACCAGCTCATTGACGTCGCGGCGAATAATCTGCgg                                                                             >  1:19515/1‑143 (MQ=255)                                                      tctgtgtttCTTCGGCCTGCGTGGCCCTGCGACCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg                                                                            <  2:488945/137‑1 (MQ=255)                                                              tcttcGGTCTTGGTGGCCTTGCGTTCGTGCCAGGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGga                                                                    <  1:67274/143‑1 (MQ=255)                                                              tctcgggTCTCTGTGGCGTTGTGACCGTGCCACGGCGTGTTTGCCGAACTGGAGGGCAGCGCCGCACGGCCAGGATGGCGCAGGATCAGCTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGga                                                                    <  2:444662/138‑1 (MQ=255)                                                               cgtcGGTCTGGGGGGCCTTGCGATCGTGCCACGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgag                                                                   <  2:503002/141‑1 (MQ=255)                                                                ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGCATAGTCTGCGGGCTGACGtaga                                                                  >  1:703960/1‑143 (MQ=255)                                                                     tCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAAAACGCCGCGAATAGTATGCGGGTTGACGGAGAAATga                                                             >  1:70010/1‑142 (MQ=255)                                                                           tGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                       >  1:540009/1‑143 (MQ=255)                                                                           tGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                       <  1:671638/143‑1 (MQ=255)                                                                                                     ttatgcGATCTGGACAGCAGCGCCGCGCCGCCATGCTGGCGCAGGCGCAGGTTCTGCCCCGCCAGCTCACTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg                             <  1:533247/138‑1 (MQ=255)                                                                                                     ttAACCGACCTTGTAGGCGGCGCCGCGCCGCCATGCGGGCGCGGGATCAGGTTTTGCTCCGCCAGCTCATCGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg                             <  1:343850/143‑1 (MQ=255)                                                                                                     ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg                             >  1:848106/1‑143 (MQ=255)                                                                                                       aaCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTGCTGACATAACCctgct                           >  1:171731/1‑143 (MQ=255)                                                                                                       aaCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCCCCTTGAGGTCGCTGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct                           <  2:638322/143‑1 (MQ=255)                                                                                                          cGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt                        >  1:568679/1‑143 (MQ=255)                                                                                                           caaCTGGAAGGCCGCGCCGCACCGCTATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt                       <  1:708232/142‑1 (MQ=255)                                                                                                            agttGGAATGCTGTGCCGGTCCGCCACGATGGCGTAGGATCGGGGTTTGCTCCGCCAGCTTATTGAGGTCGCGGCGAATAGGTTGTGGGCTTTCGGAGAAATGCTCTACCAGCTCTTCGGTACTGTCATAACCCTGCTGTTTa                      <  2:441801/140‑1 (MQ=255)                                                                                                               tGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc                   >  1:466440/1‑143 (MQ=255)                                                                                                                    ggCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCGGCTCATTGAGGTCGCGGCGAATCGTCTGCGGGCTGACGGCGAAGTGCTCTACCCGCTCTTCGGTACTGCCATGCCCCTGCTGTTTAACCGGttt              >  1:261687/1‑142 (MQ=255)                                                                                                                    ggCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTc              >  1:565501/1‑143 (MQ=255)                                                                                                                      cAGCGCCCCTCCGCCATGTGGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGCATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATACCCCTGCTGTTTAACCAGTTCGa            <  2:88161/143‑1 (MQ=255)                                                                                                                          gatcctgcGCCATCATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataat        >  2:747378/8‑143 (MQ=255)                                                                                                                                ccGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt  <  2:676321/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                  GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTT  >  NC_000913/3560316‑3560584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑