Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 124,241 C→T Q409* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913124,2410CT96.3% 65.8 / NA 27Q409* (CAG→TAG) aceEpyruvate dehydrogenase E1 component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (15/11);  minor base A (1/0);  total (16/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGC  >  NC_000913/124105‑124373
                                                                                                                                        |                                                                                                                                    
tggtCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTa                                                                                                                                >  1:755153/1‑143 (MQ=255)
tggtCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTa                                                                                                                                <  1:720803/143‑1 (MQ=255)
  gtCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTCAACACATCGCGGACTCGGTTAAg                                                                                                                              >  1:751717/1‑143 (MQ=255)
      cgctacGAAGAAAATCATCTCTGCATTCAAGAAAGCGCAGAAAACCAAAAGCAAAGCGACAGTAATCCTTACTCAAACCAGTAAAGGTTACGGCATGGGCGACTCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGaaaa                                                                                                                          <  1:55318/138‑1 (MQ=255)
      cgctCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATCCCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGaaaa                                                                                                                          <  1:323225/140‑1 (MQ=255)
           cGAAGAACATCTACGCTGCCTTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAAc                                                                                                                     >  2:554957/1‑143 (MQ=255)
                               ttCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGACCATGGACGGTGTGCGTCatat                                                                                                 >  1:840528/1‑143 (MQ=255)
                               ttCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCatat                                                                                                 >  2:192583/1‑143 (MQ=255)
                                 cAAGAAAGCGCAGTAAACCAATGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCATTAGTTTAAGAAAATGAACATGGACGGTGTGCGTCATATcc                                                                                               <  1:665892/143‑1 (MQ=255)
                                  aaGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAACACATCGCGCACTAGGTTAAGAACATGAACGTGGACGGTGTGCGTCATATCcg                                                                                              >  1:501150/1‑143 (MQ=255)
                                   aGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcgc                                                                                             >  2:562110/1‑143 (MQ=255)
                                        gcgcAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCAAACACTAGGATAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGCCCg                                                                                        >  1:294953/1‑143 (MQ=255)
                                               aaaCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAAt                                                                                 >  2:251546/1‑143 (MQ=255)
                                                    aaaGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCCACCGTCTCAATTTGcc                                                                            >  1:622570/1‑143 (MQ=255)
                                                     aaGGCAAAGCGAGACTAGTCCTTGATCACACCATAAAAGGTTACGGCATGGGCGACGCGGCGGAAGGTAAAAACATCGCGCACTAGGTTAAGAAGATGAACATGGTCGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCg                                                                           <  2:511071/143‑1 (MQ=255)
                                                     aaGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCg                                                                           <  2:755153/143‑1 (MQ=255)
                                                          aaaGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGCCCGCTTCAATGTGCCGGTGTc                                                                      >  1:235790/1‑143 (MQ=255)
                                                                   gTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTGGGTTAAAAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTGTCAATGTGCCGGTTTCCGTTGCAGa                                                             >  2:446501/1‑143 (MQ=255)
                                                                          ttGCTCATACATTCGAAGGTTACGGCAATCGCGACGCGGCTGAAGGTAAAGACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGTGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaa                                                      <  2:168966/143‑1 (MQ=255)
                                                                                      ttAAAGGTTACGGCATGGGCGACGCGGCTGAAGGAAAAAACATCGAGCACAAGGTTAAGAAAATGAACATGGTAGGTGTTCCTCATAATCAGGACATGTTCAATGTGCACATGACTGATGCCGATATCGAAACACTGCCGCAc                                          >  2:290887/1‑143 (MQ=255)
                                                                                           gtttACGGCATGACCTGCTCGTCTGAATGTAAAAACATCGCGTACTAGGTTAATAATATGAACATTGACGTTTTTCGTCATATCCTCGACCCTTTCAATGTTCCTTTGTCTTATACATATATCTAAAAACTGCCGTACATCAc                                     <  2:583004/141‑1 (MQ=255)
                                                                                            gTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGCCCGTTTCAATGTGCCGGTGTCTGATGAAGATATCGAAAAACAGCCGTACATCAcc                                    >  1:356524/1‑143 (MQ=255)
                                                                                                cGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTAAAGCCCATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAACACCGCCGTACATCACCTTcc                                >  1:120451/1‑143 (MQ=255)
                                                                                                     gggtcggcgcgGCTGAAGGGAACAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCCTATCCGCGCCCGTTTCAATCTGCCGGTGTCTGATTCAGATATCGATAAACTGTCGTACATCACCTTCCCGGaa                           <  2:791662/136‑1 (MQ=255)
                                                                                                          gACGCGGCGGAAGGTAAAAGCATCGGGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTc                      <  1:446501/143‑1 (MQ=255)
                                                                                                                 cTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAagag               <  1:251546/143‑1 (MQ=255)
                                                                                                                              cATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATACGCGACCATTTCAATGTGCCGGTGTCTGATGCAGATATCGAAACACTGCCGTACATCACCTTCCAGGAAGGTTCTGAAGAGCATACctctctcc  >  2:665904/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                    
TGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGC  >  NC_000913/124105‑124373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑