| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 96.5% | 75.9 / NA | 29 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (12/16); minor base T (0/1); total (12/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG > NC_000913/2132666‑2132924 | cAGCTGATATTTTTTGCTTGTGCAGTCGATTTTGTCTTTTTGTTTGTGGTGGACGAGCAAATACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt < 1:385364/143‑1 (MQ=255) gCTGATATTGTTTGCTGGTGCGGTCGAATTTGTCTTTGTGCTTGCGGGAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCCGCGTCGCCTCTTCTACTTTc < 1:120097/143‑1 (MQ=255) cgggggcgGTCGAGTTTGTCGTTGGGCTTGCGGTAAAAGTGGAAGTAGCCGGAGAATTTTTTCGGTGACGCGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa < 1:817796/138‑1 (MQ=255) cTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGCTCGCCggc > 2:753788/1‑141 (MQ=255) ggTCGAATTTGTCTGTGTGCTTGCGGTGAGAGTGGATGTGCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGTCTCTTCTACTTTCCACGGTTCGCCGTACTccacc < 2:318535/143‑1 (MQ=255) aaTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAAGCTCTGCATCACCTCAAATACTTTCCACGGTTCACCGTACTCCACCACCAt > 1:224920/1‑143 (MQ=255) tgttGTCTTGGTGCCTGCGGGACGAGTGGACGTCCCCGGAGAATTTTTTCGGTGACGATGTGATCTGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc < 2:595797/141‑1 (MQ=255) gTCTTTGTGCTTGCGGTAAAAGTGGAAGTATCCGGAGATTATTGGCATAAACGAGGTGATCTGCATATCACCGTGATTGATATGCAGGCTCTGCGTCGCCTTTTCTATTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa < 1:113841/143‑1 (MQ=255) gTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAACCCACCCCCATCCGCa > 2:808694/1‑143 (MQ=255) gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGTTTCGCCGTACTCCACCACCATCCGACAGAAGATGTCGTAAt > 1:606263/1‑143 (MQ=255) gtgtatgTATCCGGAGATTTTTTTCGGTGACGTGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg < 1:695743/137‑1 (MQ=255) tGGGAGCACCCGGAGAATTTTTGCGGTGACGAGTGGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGc < 2:774954/143‑1 (MQ=255) ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTGGTAATCCTGTGCCGCTGTCAg > 2:424012/1‑143 (MQ=255) gtgtgttttttCTGTGTTGATGTGATCTACATTTCACAGTGATTGATATGCACGCTATGAGTCGTCGCTACTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCACGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc < 1:429054/138‑1 (MQ=255) cGGTGACGCGGTGATCTGCATTTCTCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaaca < 2:603107/143‑1 (MQ=255) gACGGGGTGATCTGCATGTCACCGTGATTGAGATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGGAATCCTGTGCCGCTTTCAGTTCGATACCGaactgaaa < 1:808694/143‑6 (MQ=255) gACGAGGTTATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATACGCAGGAAAATGTCGTAATCCTGTGACGCGTTCAGTTCCGTAGCGaacaaaca > 2:18329/1‑143 (MQ=255) aGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCTGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGAATCGAGCAAACActct > 2:481261/1‑143 (MQ=255) ggTGCTCTGCATTTCACCGTGATTGATGTGGAGGCTCTGCGTCGCCTCTTCTACGTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt < 2:536529/143‑1 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt > 1:449209/1‑143 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGAAGACTCTGTGTCGCCTCTTCTACTTTCCACAGTTCGCCGTACTCCACCACCATCCCCAGGAAGATGTCGTGATCCTGTGGCGGTTTCAGTTCGGTAGCGACGAAACActctct > 2:297685/1‑141 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCAGCACCATCCGCAGGAGGATGTCGTACTCCTGTGCCTCTGTCTGTTCGGTATCGAACAAACACCCTTTGaa > 1:127024/1‑143 (MQ=255) gcTGCATTTCACCGTGATTGATATTCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa < 2:353445/142‑1 (MQ=255) gcGTGATTGATATGCAGGCTCTGCGTCGTCTCTTCTACTTTCCCCGGTTCGCCGGACTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTTAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCa < 2:608684/142‑1 (MQ=255) attgatATGCAGTTTCTGCGTTGCTTCTTCTACTTTTCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGGAATCCTGTGCCGCTTTCAGTTCGGTATCTAACAAACACTCTTTGAAACGCCATGCCCAGGTaa < 1:84659/143‑1 (MQ=255) ttgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCACGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGACCAAACACTCTCTGAAACGCCATGCCCAGGTaaa > 2:37080/1‑143 (MQ=255) tgagacgCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTTTGCCGCTTGCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAg < 2:178382/137‑1 (MQ=255) atGCAGGCTCTGGGGCTCCTCTTCTACTGTCCACGGTTCGTCGTACTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt < 2:164970/143‑1 (MQ=255) gCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCTCCATCCGCAGTCAGATGTCGTAATACTATGCTGCTTTCAGTTCGGTATCGAAAAAACACTCTTTGAAAAGCCATGCCCAGGTAAAGATCTgg > 1:82025/1‑143 (MQ=255) | CAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG > NC_000913/2132666‑2132924 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |