Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,397,940	G→C	N742K (AAC→AAG)	nuoG ←	NADH:quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,397,940	0	G	C	100.0%	54.4 / NA	23	N742K (AAC→AAG)	nuoG	NADH:quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (5/18);  total (5/18)

CTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCT  >  NC_000913/2397800‑2398067                                                                                                                                             |                                                                                                                                cTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAAATTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGctt                                                                                                                               <  1:510105/143‑1 (MQ=255)  tGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGCGTTCCACCCCGGCGCCCCGGCAATCGGCACTTGCGAACGGTATGCACCCGGCTGcttc                                                                                                                              >  2:537835/1‑142 (MQ=255)   gcttgcAAACAGACGCACGCCCGGATCGCCAAAGCGCAGGTTGCCGCCCACCTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCACAGTCGGCTGcttgt                                                                                                                             <  1:586464/138‑1 (MQ=255)            cAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAGCGGCACTTGCGAACGGTGCGGAGTCGGCGGCTTGTTACGTTCCa                                                                                                                    >  1:578196/1‑143 (MQ=255)                                 taatcccAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCTGCTGCTTGTTACCTTCCATCGAGAAGGTGAACATGgtgt                                                                                               <  2:260032/137‑1 (MQ=255)                                 ctaGCGCCCTTTGCCGCCCACTTCGTCCTCTAATGTGTTCCCCTCCTGCGGGGAGTTCCAGCCCGGCGTCCATTCTAACTGCACTTGCGAACGGTTCGCAGTCGGCTGCTTGTTACCTTCCATCGAGAAGGTGAACATGgtgt                                                                                               <  2:265062/141‑1 (MQ=255)                                   aGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCCGCCCCGCGCCCAGGCACACGGCACTTGTGAACGGTGCACAGTCGGCTGCTTGTTACCTTCAATAGAGAAGGTGAACATGGTGTCa                                                                                             >  1:343990/1‑143 (MQ=255)                                   aGCGCAGTTTGCCGCCCACTGCGTCCTGGAATTTGTTCCTCGACGGAGGGTATGTCCATCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGCTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCa                                                                                             <  1:577025/143‑1 (MQ=255)                                           ttGCCGCCCACTGCGTCCTGGGATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGCTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTg                                                                                     <  2:427158/143‑1 (MQ=255)                                            ggccgccCACTTCGTCCTGGAATTTGTTCCGCGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGCTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGc                                                                                    <  2:754149/142‑1 (MQ=255)                                                gccCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGACACTTGCGAACGGTGCGCAGCCGGCTCCTTGTTACCTTCCACCGAGCAGGGGACCGTCGTGCCACTATCCtcctcgc                                                                                >  2:121077/1‑137 (MQ=255)                                                       tCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGGGCGTAGTCGGCTGCTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCgg                                                                         <  2:651110/143‑1 (MQ=255)                                                       gcGTCCTGGAATTTGCTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGCTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCgg                                                                         <  1:737688/142‑1 (MQ=255)                                                               gAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGCTTGTTACCTTCCATCGAAAAGGTGAACATGGTGGAAATATCCTGCGGCTGACGCGGCTCATgca                                                                 >  1:518866/1‑141 (MQ=255)                                                                  tttGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGGCGGCTGCTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGc                                                              <  1:234203/143‑1 (MQ=255)                                                                    tGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACGTGCGAACGGTGCGCAGTCGGCTGCTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTg                                                            <  1:797926/143‑1 (MQ=255)                                                                          aCGCCTGCGGGGAGTTCCAGCCTTGCGCTTTGTCAAACGGCACTTGCGAACGGTGCGCAGTCTGCTGCTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTg                                                      <  1:424484/143‑1 (MQ=255)                                                                             ccTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGCTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATCTCCTGAGGCTGACGCGGCTCATGAACGCTGATATTGgcg                                                   <  2:743748/143‑1 (MQ=255)                                                                              cttgcgcgAGTGCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGCTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGgcgc                                                  <  2:87712/136‑1 (MQ=255)                                                                              cTGCGGGGAGTTCCGGCCCGGCGCGCAGGCACACGGCAGTTGCGAACGGTGCGCAGTCGGCTTCTTGTTACCTTCCATCGATAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTTATGAACGCTGATATTGgcgc                                                  <  2:662859/143‑1 (MQ=255)                                                                               ttcGGGGAGTTCCAGCCCGGCGCCCAGGCAAACTGCACTTGCGAACTGTGCGCAGTCGGCTGCTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTCCGGCTGACGCGGCTCATGAACGCTGATATTGgcgcg                                                 <  2:401265/141‑1 (MQ=255)                                                                                                 gtcgcCCAGGCAAACTGCACTTGCGAACGGTGCGCAGCCGGCTGCTTGTTACCTTCCATCGAGCGGGGGAACACCGTGGCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCt                               <  1:715141/141‑1 (MQ=255)                                                                                                 ggCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGCTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCt                               <  1:419188/143‑1 (MQ=255)                                                                                                 ggCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGCTTGGTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCt                               <  1:827923/143‑1 (MQ=255)                                                                                                         ggCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGCTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGCCGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACTACCGCTGTAACAGt                       >  1:676422/1‑143 (MQ=255)                                                                                                                              gggcgcGGTCGGCGTCTTGTTACCTTCCGTCGAGAATGTGAACATGGTGTCAATGTCCTGCGGCTGACGCGGCTTATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCt  <  2:10773/141‑1 (MQ=255)                                                                                                                                             |                                                                                                                                CTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCT  >  NC_000913/2397800‑2398067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑