| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,661,821 | C→G | C104S (TGC→TCC) | iscR ← | DNA‑binding transcriptional dual regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,661,821 | 0 | C | G | 94.4% | 93.5 / NA | 37 | C104S (TGC→TCC) | iscR | DNA‑binding transcriptional dual regulator IscR |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (16/18); minor base T (1/1); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCAC > NC_000913/2661686‑2661956 | cGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATTCAGCTCTTTCTGGTTATTAATCAGTTCGTCTAAATTTTTGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATc < 1:445358/143‑1 (MQ=255) gTATGCTGACGGCCAGACACACCCAGCACTTGCTGTTTATTAACCAGTTCGCCTAAATTAATGTTGTTGAGAAACCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAgcc < 2:783708/143‑1 (MQ=255) aTGCTGACGTCTAGACTCATCTAGAAGTTCTTTGTTATTAACGTGTTCGCCTTATGTTATGTAGTTAAGAAAGTCGTTGAGACGGTCGCTCAAATCACGCCACAGCGCTTTTGTCAGTTATTTATCGCCGCCCTGGCAgccgc < 2:689633/143‑1 (MQ=255) aTGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAgccgc < 2:338843/143‑1 (MQ=255) cTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTCAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCAACAGCGAGTAGGACAGGAATTTATAACCGCCCTGGCAGCAGCCtt > 1:97143/1‑143 (MQ=255) cTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTACCGCCGCCCTGGCCGCCGCCtt > 2:160211/1‑143 (MQ=255) gacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTAAGGGATGTATCCCCGCCCTGGCAGCCGCCTCTAccc > 2:568629/1‑143 (MQ=255) gacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGGATTTATCGACGCCCTGGCAGCCGCCTTTAgcc > 2:661847/1‑143 (MQ=255) acCAGACACATCCAGCACTTCATGGTTATTAACCAGTTCGCCTAATTTTATGTTGTTGAGTAATCCGTTTTTACTGTTTCTTATTTTACGCCTCAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCt < 1:113135/143‑1 (MQ=255) tggACACATCCGGGCCTGTCTGGTGATTAACTGGTTCGGCTAGAGTAATGTTGTTGAGAAAACGGGTGAGACGGTCGCTCACATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGa < 2:345913/141‑1 (MQ=255) tCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGt > 2:698415/1‑143 (MQ=255) aGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGc < 2:395895/143‑1 (MQ=255) aCTTCCTGGTTATTAACCAGTTCGCCTCAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTGAAATCACGTCACAGCGCGTGGGGCAGGGTTTTATAGCGGCCCTGGCAGCGGCCTTTAGCCTGACAACGGGTCGCATc > 2:543725/1‑143 (MQ=255) ttattaACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACAGGTGAGACGGTCGCTCAAATCACGACACAGCGCGTGGGTCAGGGATTTATCGCCGCCATAGCAGCCGACTTTACCATGACAAAGGGTTGCATCTACAGATTc > 2:140492/1‑143 (MQ=255) aCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTAGCATCTACAGATTCATCAAc > 1:536080/1‑143 (MQ=255) ccTAAAGTAAGGTTGTTTAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCGGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAAt < 2:307015/143‑1 (MQ=255) cTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGACCTGGCAGCCGCCTTTACCCTGGCCACCGGTTGCATCTCCAGATTCGGCACCGGCGCTACtt > 2:97986/1‑143 (MQ=255) tAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACAGCGCAAATTa > 1:549143/1‑143 (MQ=255) gttgttGAGAAAACCGGTGAGAAGGTCGCTCAAATCACAACACAGCGCGTGGGTAAGGGATGTATCGCCGCCCTGGCAACAGCCTTTAACCTGAGAACGGGAGGCATCTACAGAATCGTCAACAGCAGTAAGTACTTGTCCaa > 2:432350/1‑143 (MQ=255) tgttgAGAAAAACGGAGAGACGGTAGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACg < 1:327436/143‑1 (MQ=255) ttgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCGACGGCGCTAATTACTTAGCCaaacgc > 1:725693/1‑139 (MQ=255) ttataacaCCTGTGAGACGGTCGCGCAAGACACGACACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCg < 1:645882/136‑1 (MQ=255) gaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGTCCTAATTACTCCGCCACCGGCGAtg > 1:724676/1‑143 (MQ=255) aaaaCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAtgc > 1:600561/1‑143 (MQ=255) cGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGctgctg < 1:698415/143‑1 (MQ=255) tGAGACGGTCGCTCAAATCACGCCACATCGCGTCGTTGAGGTATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGCCGATGCTGCTGGcc > 1:855010/1‑142 (MQ=255) cGGTCGCGCAAATCACGCCACGGCGCGTGGGTCAGGGATGTAGCGCCGCCCTGGTTGTCGCCGTCACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCttt < 1:191114/143‑1 (MQ=255) gTCGCTCAAATCGCGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGc < 2:844060/143‑1 (MQ=255) gTCGCGCAAATCACGCCACAGCGCGGGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGc < 1:144884/143‑1 (MQ=255) agcGCACAAATCACGCCACAACACAACCATCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGc < 1:344960/141‑1 (MQ=255) ttttaTCACGCCACAGGGCGTGGGTCAGGGATTTAGCGCCGCCGTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAAc < 1:170046/139‑1 (MQ=255) tCAAATCACGCTACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCAGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAAc < 1:177394/143‑1 (MQ=255) gagtCACGCCCCAGCGCGTGGGTCCGGGATCTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTTGCATCTACGGCTTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAg < 2:770527/140‑1 (MQ=255) aTCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTGTGCCTACCAGac > 2:160846/1‑142 (MQ=255) aGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAGCCTGCTGGCCTCTTTGCCTCACAGATAACCGCGGcc > 2:486014/1‑143 (MQ=255) gTGGGTCAGGGATTTATCGCCGCCCGGGCAGCCGCCTTTACCCGAACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAAATACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTc < 2:657715/143‑1 (MQ=255) ggTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATAACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAAACACCGCACGGTcccc > 2:322623/1‑141 (MQ=255) | CGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCAC > NC_000913/2661686‑2661956 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |