Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,634	A→C	T108P (ACC→CCC)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,634	0	A	C	97.8%	118.3 / ‑6.7	47	T108P (ACC→CCC)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (24/21);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGT  >  NC_000913/3041496‑3041772                                                                                                                                           |                                                                                                                                           gaTTGCGACGCGAAAGGTAAAACGTGGAGCAAGTTACGTCTGTTCCGCGACGGCGATGGCTGTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCGGGCTGCACTGAAAAAATATGCGGTATGCTCTAAAGTGCCCAt                                                                                                                                        <  2:219384/142‑1 (MQ=255)   ttGCGGCGCCGACGGTAAAATGTGGGGAAATTTAGGTGTGTTCAGCGCCGGCGATGGCTTTGCACGGATTGAACCGCGAAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATcg                                                                                                                                      <  2:656761/143‑1 (MQ=255)      cGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCgcgc                                                                                                                                   <  1:329209/143‑1 (MQ=255)            cAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAgacg                                                                                                                             >  1:583092/1‑143 (MQ=255)             aaaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGGCTTCTCTAAAGTGCCCCACGCCCCAgacga                                                                                                                            >  2:730751/1‑143 (MQ=255)                     aaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCggg                                                                                                                    >  2:17312/1‑141 (MQ=255)                              cgtttgaCGTCTGTTGCGCGCCTGCGATGGCTTTGCATCGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGTAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                                                                           <  2:777030/137‑1 (MQ=255)                               aaTTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGCACGGCGCACCATGCGTGAACCGCAGCTGGCTGAACTGAAAAAAAATACGATATTCTCTAAAGTACCCATCGAGCCAGACGACGAGCGTGTACTGATTga                                                                                                          >  1:432859/1‑142 (MQ=255)                                aTTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAATGCCAATCGCGCCAGACGACGAGCGTGTACTGATTGgt                                                                                                         >  1:357087/1‑143 (MQ=255)                                  ttACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtgt                                                                                                       >  2:73593/1‑143 (MQ=255)                                       tCTGTTCCGCGACGGCGATGGCTCTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg                                                                                                  <  1:73593/143‑1 (MQ=255)                                         tGTTCCGCGACGGCGATGGCTTTGAATGGATTGAACGGCGCAGGGTGCGTGAACCGGAGCTGACGGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGACAGACGATGAGCGTGAGCGGCTTGGTGTTCCGGGt                                                                                                >  2:329375/1‑143 (MQ=255)                                                cgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCAATCGCGCCCAACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTAAgg                                                                                         >  2:369312/1‑143 (MQ=255)                                                    ggCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGCGCGTGTGCTGCTTGGTGTTGCCGGCTATCAGgcgcg                                                                                     >  1:648389/1‑143 (MQ=255)                                                          ggCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCCTCGCGCCGGACGACGAGCGTGTGATGCTTGGTGATGCAGGATTTCAAGCGcgcgccgc                                                                               >  1:22239/1‑143 (MQ=255)                                                                      aTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGCTGCCGTTGCTCGGCCGCGCGCCGTGTAGACAAAtct                                                                   >  1:109497/1‑143 (MQ=255)                                                                        tgaccagcgcAGTGAGAGTGAACCGCAGCTGACTGAACTGCAAAAAGATGCGGTATTCTCTAAAGTGCCCATCGAGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct                                                                 <  1:495319/137‑1 (MQ=255)                                                                                gcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGaa                                                         >  1:792590/1‑143 (MQ=255)                                                                                 cAGCGTGCGTGAACCGCAGCTGAGTGAACTGAAGAAATATGCTGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAAc                                                        <  2:452384/143‑1 (MQ=255)                                                                                   gcgtgggtGACCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGTCGCGCTGGCAAATCTCTTTAGCGAACTg                                                      <  2:493604/143‑1 (MQ=255)                                                                                    cgtgcgtgAACCGCAGCTGACTAGACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGc                                                     <  1:729157/143‑1 (MQ=255)                                                                                      tgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCCCCGCGCTGGCAAATCTCGTTAGCGAACTGCCt                                                   >  1:271798/1‑143 (MQ=255)                                                                                        cttgAATCTCATCTGACTGATTTGAAAATATATGTGGTATTCTCTAAAGTGCCCAGCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTc                                                 <  1:494615/141‑1 (MQ=255)                                                                                        cgtgAACCGCAGCTTATTGAACTGAAAAATTATGCTTTATTCTCTAAAGTGCCCATCGGGCCAGACGAGGAGCGTGGGCGGCGTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTc                                                 <  1:140666/143‑1 (MQ=255)                                                                                          tgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCga                                               >  1:513098/1‑143 (MQ=255)                                                                                           taaCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaa                                              <  2:520898/142‑1 (MQ=255)                                                                                            aaCCGCAGCTGACTTAACTGAAAAAATATGCGTTATTCTCTAAAGTGTTCATCTCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTGTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa                                             <  1:211534/143‑1 (MQ=255)                                                                                            aaCCGCAGCTGACTGAACAGAAAAAAGATGCGGTATTCTCGAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa                                             <  1:132726/143‑1 (MQ=255)                                                                                               ctgaGCTCACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTTgaaagaa                                          <  1:542351/140‑1 (MQ=255)                                                                                               cGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATGGCGCCAGAAGACGAGCGTGTGCTGCTTGGTGTTGCCGGTGTTCAGGCGCGCGCCCAGCTGGCAAACCTATTTACCGAAGTGCGTTCgaagacg                                          >  2:772830/1‑139 (MQ=255)                                                                                                 taGCTGATTGAAATGAAAAAATATGCGTTATTCTCGAAAGGGCCCATCGTGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGaaaa                                        <  2:367032/142‑1 (MQ=255)                                                                                                    ctgactgaACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTTGCGAGCTGCCTTCGAAAGCAAcaca                                     >  1:489876/1‑143 (MQ=255)                                                                                                     tgactgacCCGAAAAAATAGGCGGTATGCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAg                                    <  2:772044/143‑1 (MQ=255)                                                                                                           aactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTc                              >  1:406794/1‑143 (MQ=255)                                                                                                           aactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCACACGACGAGCGTGTGCTGCTTGGTGGTGCACGCTATCAGACGCGCACCGAGCTCACAAAGATCTTTATCCAACTGCCCTCGCAAGAAAAACacctacac                              >  2:722788/1‑136 (MQ=255)                                                                                                              tgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCCTCGAACGAAAAACAGGTACTCaca                           >  2:389336/1‑141 (MQ=255)                                                                                                               gaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATATCTTTAACTAACTGCCTTCGAAAGAAAAACAGGTAGTCAaag                          >  1:658315/1‑143 (MQ=255)                                                                                                               gaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCCAGAAACACACCGAGTCTTTTTGCCTTCGAAAGAAAAACACGTAGGCAaag                          >  2:596233/1‑143 (MQ=255)                                                                                                                    gatctGCGGTATTATTTAGACTGCCCATAGCGCCAGTCGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgc                     <  2:287130/139‑1 (MQ=255)                                                                                                                    aaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGAGGGTTAACACCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgc                     >  1:386653/1‑143 (MQ=255)                                                                                                                        tGCGGTACTCGCGAAAGCGCCCCTCGCGCCAGACGACGAGCTTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCGTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGa                 <  1:17312/143‑1 (MQ=255)                                                                                                                           ggTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCGGCTCTGGCAAATGTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCa              >  2:53940/1‑143 (MQ=255)                                                                                                                           ggTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTAAAAGAAGGAGCGACCa              >  1:561654/1‑143 (MQ=255)                                                                                                                                tctcTAAATTGCCCATCGCGCCAGACGACTAGCGTGTGCTGCTTGGTGTTGCCTTTTTTCAGGCTCGCGTCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTg         <  1:227262/143‑1 (MQ=255)                                                                                                                                 ctctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAGTCTCAGGTAGTCAAAGAAGGCGCGACCACTTTGc        <  2:513098/143‑1 (MQ=255)                                                                                                                                 ctctAAAGTGCCAATCGCGCCAGACGACGAGCGTGTGCAGCTCGGTGTTGCCGCTCTCCAGCCCCGCGCCGCGCTGCCAAATCTCTTTAGCGAACAAAAAAAAAAAAAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGc        <  2:540265/143‑1 (MQ=255)                                                                                                                                       aGTGACGACCGGGCCAGACGACGGGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGt  <  2:581035/143‑1 (MQ=255)                                                                                                                                           |                                                                                                                                           CATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGT  >  NC_000913/3041496‑3041772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑