Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913	3,815,859	Δ82 bp	100%		[rph]	[rph]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3815859	3815940	82	27 [0]	[0] 27	[rph]	[rph]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3815858	0 (0.000)	25 (0.820)	22/244	NT	100%	intergenic (‑90/+5)	pyrE/rph	orotate phosphoribosyltransferase/truncated RNase PH
	?	NC_000913	3815941 =	0 (0.000)					coding (609/687 nt)	rph	truncated RNase PH

TTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT  >  NC_000913/3815725‑3815997                                                                                                                                       |                                                                                                                                            ttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctg                                                                                                                                    <  1:105483‑M1/143‑10 (MQ=255)         cgcAAACTCAATAACCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaa                                                                                                                            <  1:131086‑M1/143‑18 (MQ=255)                 cAATAACCTGGCGCTAATATGTTTTCATGCCTTCGCTCCTCATCTTACTTTCCCACCGAC‑GAAAAAAACGCGACACCTCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgc                                                                                                                    <  2:640713‑M1/143‑26 (MQ=255)                  aaTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAGAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcc                                                                                                                   >  1:511537‑M1/1‑117 (MQ=255)                   aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcggcccctgcacttcaatgctgcgccc                                                                                                                  >  2:290694‑M1/1‑116 (MQ=255)                   aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGACAGCGCCGCCTCATGCcgtcccctgcgattcaatcagccgccc                                                                                                                  >  2:182031‑M1/1‑116 (MQ=255)                         tGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACCGAC‑AAAAAACAGGCGACTCATCAGTCGCGTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcattgatgcgaccatcgtt                                                                                                            >  1:329095‑M1/1‑110 (MQ=255)                                  tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcgggcatcac                                                                                                   >  2:372158‑M1/1‑101 (MQ=255)                                    tGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcacta                                                                                                 <  1:560179‑M1/143‑45 (MQ=255)                                            tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactccgttcat                                                                                          >  1:580735‑M1/1‑92 (MQ=255)                                            tGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAACAGGCGACTCATCAGTCGCCTTCAAAATCAGTTTGCCAGCGCCCCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactaggttcatg                                                                                         >  1:571446‑M1/1‑91 (MQ=255)                                               cTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTCTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcg                                                                                      <  1:519766‑M1/143‑56 (MQ=255)                                                   gCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCTCCGCCTTCTGCcgtcccctgcacttcattgatgcgcccgtcttcggtcatcactacgttcatgtcggtct                                                                                  <  1:248550‑M1/143‑60 (MQ=255)                                                           tCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggca                                                                          >  1:374060‑M1/1‑76 (MQ=255)                                                                 ttttCTACAGAC‑AAAAAAAAGGCGACTCATCAGGCGCCTTAAAAATCAGATTGCCAGCGCCGCCTTCTGCcgacccctgcacttcaatgatgcgcccgtcttcggtcctccctacgttcatgtcgcgctctgcggcagcgcct                                                                    >  2:270654‑M1/1‑70 (MQ=255)                                                                   ttCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccggcttcggtcatcactacgttcatgtcgggctctgcggcagagtcttc                                                                  >  1:50748‑M1/1‑68 (MQ=255)                                                                    tctcCAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttca                                                                 <  1:375211‑M1/143‑77 (MQ=255)                                                                              aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattcc                                                        >  1:657470‑M1/1‑58 (MQ=255)                                                                               gaaaaaagGCGCCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtctgcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattcca                                                       <  1:137479‑M1/142‑87 (MQ=255)                                                                               aaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattcca                                                       >  1:356629‑M1/1‑57 (MQ=255)                                                                               aaaaaaaGGCGACTCATCAGTCGCATTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcaccactacgctcctgtcggtctctgcggccgagtctccaacgtatccca                                                       >  2:453041‑M1/1‑57 (MQ=255)                                                                                                   tCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaccgtattccagatcgcaaacccctccgccg                                   >  2:277543‑M1/1‑37 (MQ=255)                                                                                                       cTTAATAATCACTTTCCCAGCGCCGCCTTCTGCcgccccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcaaaccgcttcgccgttca                               <  2:430603‑M1/143‑111 (MQ=255)                                                                                                        ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattcccgatcgccaaccgcttcgccgttccc                              >  1:316628‑M1/1‑32 (MQ=255)                                                                                                                       ccAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcaaaccgcttcgccgttcacaattccgacagaaac               >  1:12043‑M1/1‑17 (MQ=255)                                                                                                                           cgccgcCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcaaaccgcttcgccgttcacaattccgacagaaactgcg           <  2:374060‑M1/143‑131 (MQ=255)                                                                                                                                    cTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcctcactacgttcacgtcggcccctgccgcagagtcttcaacgtattccagatcgcaaaccgcctcgccggtcacaactccgaccaaaacggaggctacaatc  >  2:209232‑M1/1‑4 (MQ=255)                                                                                                                                       |                                                                                                                                            TTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT  >  NC_000913/3815725‑3815997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑