| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 83,590 | G→T | 12.9% | intergenic (‑61/+32) | leuA ← / ← leuL | 2‑isopropylmalate synthase/leu operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 83,590 | 0 | G | T | 12.9% | 86.5 / 3.5 | 38 | intergenic (‑61/+32) | leuA/leuL | 2‑isopropylmalate synthase/leu operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base G (16/16); new base T (2/3); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 | |||||||||||
CACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGA > NC_000913/83455‑83726 | cacaCTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCggg < 2:157952/143‑1 (MQ=255) cTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCGTTTAGAGCGCCTCGCTTCGGGCATCAAAACACCCGCGCAATGGCGCGGGtttt > 1:211413/1‑143 (MQ=255) tccggCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTAGCGAGAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCGTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttttt < 2:603772/139‑1 (MQ=255) tCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCGATGGCGCGGGttttt > 2:273865/1‑143 (MQ=255) tCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttttt > 2:266441/1‑143 (MQ=255) cAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGtttttt > 1:643246/1‑143 (MQ=255) cAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGtttttt < 1:612213/143‑1 (MQ=255) ggcgggCCTGGAACGCGTGTGCACCGTCGCGCAGTGTGGTATCGAAAATAATGACTTGGTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgt < 1:269505/138‑1 (MQ=255) cTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgttt > 1:461247/1‑143 (MQ=255) gCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGAc > 1:269708/1‑143 (MQ=255) tCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTaa < 1:557655/143‑1 (MQ=255) cgcAATGTGGTATCGAAAATAATGACTTGCTGGCTCATAGTTTGGGTCCTGGTCTCTTTTAGAGCGCCTAGCTTCGGGGATAAAAAAACCCGCGCAATGGTGCGGGGTGTTTGTGTGGCTGCGTGGTGGCTGAATGATggtgg > 1:275512/1‑140 (MQ=255) tCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGt < 1:266441/143‑1 (MQ=255) tCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCGTAATGCTGGATGCCGCTCACTCGt > 1:309374/1‑143 (MQ=255) cTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaag > 2:221479/1‑143 (MQ=255) ttGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATACCGCTCACTCGTCTACCGCGCAaaga < 1:511544/143‑1 (MQ=255) ttGCTGGCTCATGGTTTGGGCCCTTGTCTCTTTTAGAGCGCCTCGCTTCGCGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaaga < 1:511581/143‑1 (MQ=255) tGCTGGCGCATGGTGTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaagaa < 1:267777/143‑1 (MQ=255) tGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGGTGCCGCTCACTCGTCTACCGCGCAACGACGAt > 2:448694/1‑143 (MQ=255) ggtCATGGTTTGGGTCCTTGTCCCTTTCAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGc < 1:274921/141‑1 (MQ=255) catgcgttGGGGCCTTGGCTCTTTGAGAGCGCCGCGCTTGTGGCATAGAAAGACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGtt < 2:158101/137‑1 (MQ=255) ggTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAGAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtag < 2:4350/143‑1 (MQ=255) ggTTTGGGTCCTTGGCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAGGCCCGCGCGATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATTCTGGATGCCGCTCACTCGTCGACCGCGCAAAGAAGATGCGTTtag < 1:81405/143‑1 (MQ=255) tCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagta > 1:439394/1‑143 (MQ=255) ttGTCTCTTTTAGAGCGCCTAGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAGAGAAGATGCGTTTAGTAGTAGTAGAc > 2:160741/1‑143 (MQ=255) ggtggctTTTAGGGCGCCGCGCTTCGGGCATAAAAAGAGCCGCGCAATTGCGCGGGTTTTTTGTTTGCCTCCCTTCTTGCTTACTGCTGGGTGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGAcc < 2:232550/138‑1 (MQ=255) tctcTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGCCCGa > 1:39177/1‑143 (MQ=255) tctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATa < 2:82068/143‑1 (MQ=255) tctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATCCCGCTCCCTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGCTa > 1:24628/1‑143 (MQ=255) gtttAGAGCGCCTCGCGTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATaaa < 1:40630/142‑1 (MQ=255) gcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGAt > 1:576834/1‑143 (MQ=255) aTAAAAAAACCCGCGCAAGGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAAt < 1:400394/143‑1 (MQ=255) tAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGGTGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATc > 2:550456/1‑143 (MQ=255) aaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCACTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAg > 1:119549/1‑143 (MQ=255) aagaCCCGCGCAAGGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCt < 1:159835/140‑1 (MQ=255) aCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCa > 2:178477/1‑143 (MQ=255) cgcgCAATTGCGCGGGTTTTTCGTTTGACTGCGTGCGGGCTTAATACTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGAt < 1:211073/143‑1 (MQ=255) cgcAATCGCGCGGGTCTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGa < 1:171930/143‑1 (MQ=255) | CACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGA > NC_000913/83455‑83726 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |