| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,876,107 | G→A | 11.8% | M145I (ATG→ATA) | nimT → | 2‑nitroimidazole exporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,876,107 | 0 | G | A | 11.8% | 80.5 / 3.2 | 34 | M145I (ATG→ATA) | nimT | 2‑nitroimidazole exporter |
| Reads supporting (aligned to +/- strand): ref base G (18/12); new base A (2/2); total (20/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
TCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAAC > NC_000913/1875965‑1876246 | tcCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCCGACTTACCGGCGCATATTCCCTGACAATg > 2:235763/1‑143 (MQ=255)ctgtgtCGCCGTCCTTGTGATTTGGCGGTCCAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATCTCTCACTGCCAGGATTAATTGAACGTGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATg < 2:487594/138‑1 (MQ=255) tacttaTTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGc < 2:500901/143‑1 (MQ=255) ttGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGCGACTgcgg > 1:54180/1‑140 (MQ=255) cGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGAGTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGCTCgg > 1:93309/1‑143 (MQ=255) cGGTCAGGGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATCAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTc < 1:88243/143‑1 (MQ=255) ggCGCTGGGACTGCATTCGGCTGGGTCTTACTGCCAAGATCAATTGAACGCGATTTCCCTCATTCCGTCGTCGGACTTACCGGCGCATATTTCCTGGCAATGGGAGCTGCTGCGGCACTGGGCTCGGCTATGGTCGTGCCGCt < 2:344301/143‑1 (MQ=255) gggATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCttt < 1:656756/143‑1 (MQ=255) aTTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGtt > 2:543491/1‑143 (MQ=255) ttAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACACTGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGttt > 1:130296/1‑143 (MQ=255) aaTGTCTTACTGCCAGGATTAATTAAACGCGGTTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTtggctg > 1:379580/1‑143 (MQ=255) cAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATAGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTg > 2:204665/1‑143 (MQ=255) cAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATAGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTg > 2:204762/1‑143 (MQ=255) aGGATTAATTAAACGCGATGTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGc > 2:539668/1‑143 (MQ=255) ggATTAATTAAACGCGATTTCCCTCATGCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCt < 1:235605/143‑1 (MQ=255) attaattaAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCa > 1:12455/1‑143 (MQ=255) taattaaACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATg > 1:338778/1‑143 (MQ=255) taaACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGa > 1:372249/1‑143 (MQ=255) gcgATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGCCTTTGAACGGCTTCGGCTGGCCAGGCGCGTTGCTCCTTCTGAtcggt > 1:37859/1‑139 (MQ=255) gATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGttg > 1:538546/1‑142 (MQ=255) aTTTCCCTCAGGCCGTCGCCAGACGTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGtttt < 1:124912/143‑1 (MQ=255) tttCCCTCCTTGCGTGGCCTGACTTGCCGGCGCATATTGCCTGACGGTGGGAGCTGCAGCGGCACTGGGATCGGCTGTGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTc < 2:15444/143‑1 (MQ=255) tttCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTc > 2:15506/1‑143 (MQ=255) cccTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCctc > 2:304767/1‑143 (MQ=255) cgtgaTTCGGTCGCCCGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCCGCACTGGGATCGGCTATGGTCGTGCCGCGGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCctct < 1:480927/139‑1 (MQ=255) ccAGACTTACCGGCGCAGATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGACTGTCAAGTCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCttttt < 1:416345/143‑1 (MQ=255) cTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATg < 1:543491/143‑1 (MQ=255) ttACCGGCGCATATTCCCTGACAATAGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATgg < 1:204665/143‑1 (MQ=255) ttACCGGCGCATATTCCCTGACAATAGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTCCTGGCTCTTTTTTTATgg < 1:204762/143‑1 (MQ=255) cGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGCACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGc > 2:174222/1‑143 (MQ=255) cGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTGTCCCGCTGCTGGCGCTTGTGTCAGGGCTGc > 2:248629/1‑143 (MQ=255) ttCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTCAACGGATTTGGCTGGCAAGGCGCGGTGCTCATGCTGATGTGTTTTCCTCTGCTGACGCTTATTTTATGGCTGCCACAGTgtc > 2:361061/1‑141 (MQ=255) ccTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCGAGGCGCGTTGCTCATGCTGATGGGTTGTCCTCTGCTGGCTATTTCTTTATGGCTGCCGCAGTGGCGaa > 2:584798/1‑143 (MQ=255) ccTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGaa > 1:487474/1‑143 (MQ=255) ccTGACAATGGGAGCTGCAGCGGCACTGGGAGCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGaa < 1:289420/143‑1 (MQ=255) aaTGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTcaac < 2:338778/143‑1 (MQ=255) | TCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAAC > NC_000913/1875965‑1876246 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |