| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,032,336 | G→C | 11.5% | intergenic (‑19/+48) | dcm ← / ← yedJ | DNA‑cytosine methyltransferase/putative HD superfamily phosphohydrolase YedJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,032,336 | 0 | G | C | 11.5% | 92.0 / 4.9 | 35 | intergenic (‑19/+48) | dcm/yedJ | DNA‑cytosine methyltransferase/putative HD superfamily phosphohydrolase YedJ |
| Reads supporting (aligned to +/- strand): ref base G (18/13); new base C (2/2); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01 | |||||||||||
TACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCG > NC_000913/2032198‑2032474 | tACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCGGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGAt < 1:433523/143‑1 (MQ=255) cATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTCAGATGGCCg < 1:27920/143‑1 (MQ=255) cATTAAGCTGCGCCACCAACGTTTTAACATCAGAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTCAGATGGCCg < 2:512507/143‑1 (MQ=255) ttAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGACCGAc > 2:622595/1‑143 (MQ=255) gCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGt > 1:437873/1‑143 (MQ=255) ccaccaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTCCATCGATTTCACCGGCCATCTGATATGGCAGACAGGTTAACaa > 1:461224/1‑142 (MQ=255) aCGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATATATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTAc < 2:46420/143‑1 (MQ=255) cgctttGACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACa < 2:245858/140‑1 (MQ=255) cGTTTTAACATCATACATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATGTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACa < 2:506361/143‑1 (MQ=255) catcatAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTa > 1:656559/1‑143 (MQ=255) tcatAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACGGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAgg < 2:631237/143‑1 (MQ=255) aaaTTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCCTAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATCATTAGAGACTTTTAaggtga > 1:405646/1‑140 (MQ=255) aaTTTGCATCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCAc < 1:117603/143‑1 (MQ=255) tctcCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCg < 2:651323/143‑1 (MQ=255) tcCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGa > 1:438536/1‑143 (MQ=255) ccTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCa < 1:185423/143‑1 (MQ=255) tgtgCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAgg < 1:267209/86‑1 (MQ=255) tgtgCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAgg > 2:267209/1‑86 (MQ=255) gCGGCACTCCCGGTGCTGGATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTAt < 1:75696/143‑1 (MQ=255) ccGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTCAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGCAAACGCGTCTATTACCTtgtg > 2:298690/1‑143 (MQ=255) ccGGTGCTGTATGAATCGGTCACTGTTCTATTTTCCTGCATAGATTTCCCCGGCCACCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTtgtg < 2:411747/143‑1 (MQ=255) ggTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCCCGCGCTCCGCCCGCGGATGAAAGCGCATCTATCAACTTGTGaa > 1:116695/1‑142 (MQ=255) cTGTGTGAATCGGGTTGTGATATAGTTCCCTGGATTGATTTCACCGGCCTTCTGAGGTGGCCGACGGGATAGCGTAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGac < 2:167141/143‑1 (MQ=255) tGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCg > 2:411739/1‑143 (MQ=255) tGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCg > 2:179886/1‑143 (MQ=255) gCATAGATTTCCCCGTCGATCTGGGATGGGCGACAGTTTAACATAATTACAGCTATTTAGGGCCACGCGCTTAGTCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACATCTTCATTCTCCCCCGCCAGTTCGGCACTg < 2:67995/143‑1 (MQ=255) aTAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAg > 2:45242/1‑143 (MQ=255) gATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCtt > 2:102495/1‑143 (MQ=255) tttCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCACTCTCCCCCGCCAGCTCGGCACTGAGCTCCg > 2:308099/1‑143 (MQ=255) tCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGGCACCTTCATCCTCCCCCGCCCGTTAGACCCTGAGCTTCGcc > 2:112858/1‑143 (MQ=255) cGGCCATCTCAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCAtga > 1:145064/1‑141 (MQ=255) ggCCATCTCAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCCGCACTGAGCTTCGCCATaaa > 2:287217/1‑143 (MQ=255) gCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATacac > 1:247666/1‑143 (MQ=255) ccATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGACACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCGCGCCCAGTTCTGCACTTAGCATCGCCACAAAcg > 2:653163/1‑142 (MQ=255) aTCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCCCTGAGCTTCACCATAAACTCg > 1:580911/1‑143 (MQ=255) | TACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCG > NC_000913/2032198‑2032474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |