| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,368,101 | T→C | 100% | A21A (GCT→GCC) | arnA → | fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,368,101 | 0 | T | C | 97.1% | 66.0 / ‑6.0 | 26 | A21A (GCT→GCC) | arnA | fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base C (16/8); minor base A (0/1); total (16/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTGTGG > NC_000913/2367963‑2368239 | tCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGACTAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGccgccg > 1:535574/1‑143 (MQ=255) ggtgcccccccCCGCTCTTTTGTTCAGCAAGTTATCCGTCCCTCCAGCATGGAAAATGAATAATTAAAACCGTCGTTTTTGCCTATCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAgc < 1:480773/140‑1 (MQ=255) ttttGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATAc < 2:331573/143‑1 (MQ=255) ttctttcgCAACTTATCCTGCCATCAACGAAGGAGACTGACTAATGACAACCGTTGTTTTTGCCTCCCACCATTAGCGTTGCCTCGTTATTGAAGCCCTGCTTGCTGCCGTTTATGAATTTAGCGCCATTTTTACCCATACTg < 2:658712/136‑1 (MQ=255) cAGCAAGTTATCCGTCCATCCAGCAAGGAAACTGAATAATGAAAACCGTCGTTTTTGCCTACCACGCTCTGGGCTGCCTCGGTATCGAAGCCCTGCTGGCCGCCGGTTACGAAAATAGCGACATTTTTACAAATACTGaaaaa > 1:191702/1‑142 (MQ=255) gCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGGTACGCAGTTAGCGCCATTTTTACCCATACTGATAATcc > 1:490336/1‑143 (MQ=255) gTCCATCCAGCAAGGAAAATGAATGATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGCAGCCCTGCTGGCCGCCGCTTACGAACTTAGCGCCATTTTTACCCATACTGATAAACACGGTaaaaaaa > 1:13860/1‑142 (MQ=255) ggAAAATGAAGAATGAAAACCGTCGTTTTTGCCTACCTCGATATGGGATGCCTCTGTATTGAAGCCTTGTTGGTCGTCGGTTACGAAATTAGCGCCAATTATAGCCATACTGATAAGCCCGGTGAAAAAGCCTTTTATGGTTc < 1:69883/143‑1 (MQ=255) tGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTCCGAAATTAGCGCTATTTTTACACATAATGATAATCACGGTGAAAAAGCGTTTTATGGTTCGGTGGc > 1:388183/1‑143 (MQ=255) aaCCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTTCTCTTACTGATAATGCCGGTGAAAAAGCCTGTTATGGTTCGGTGGCTCGTCTggcgg < 1:557041/143‑1 (MQ=255) tcgtTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGaa > 2:451227/1‑143 (MQ=255) gtttgGCCTACCACGATATCGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGATATTATCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTGGATGGTTCGGTGGCTCGTCTGGCGGCGGAAaga < 1:130984/142‑1 (MQ=255) tGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCATTGTGTGGTTCGGTGGCTCGTCTGGCGGCGGAAACAGGCa > 1:191985/1‑143 (MQ=255) tACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTcc > 2:392611/1‑143 (MQ=255) tACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGCCGGGCAGAGGCGTTcc > 1:82145/1‑143 (MQ=255) aCGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGCAAGAGGCATTCCGGtt > 1:380856/1‑143 (MQ=255) ttatGGGATGCCTCGGTATTGAAGCCCTGCTTGCCGCCGGTTTTGTAGTTAGCCCCATTTTTTTTCTTACTAATAAACCCGGTGAAAAAGCCTTTTATGGATCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTAt < 1:122789/142‑1 (MQ=255) tGCCTCGGTATTGAAGCCCGGCTATCCTCTGATTACGATAGTAGTGGCATTATCACCCACACTGAGAATCGCTGTTGAAAAGCCTTTGATGGGGCGGTTGGTTGTCTGTCGTCGGACAGGGGCATGGGGGTTTATGCGCCGGa > 2:435682/1‑143 (MQ=255) cTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATac > 2:380961/1‑142 (MQ=255) cTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATaa > 1:275886/1‑143 (MQ=255) tCGGTATTGAGGCCCTGCTGGCCGCGGGTTACGAAATTAGCGCCATTTTTACCCAGGCTGAGAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGTCATTCCGGTTTATGCGCCGGATAAc < 2:527313/143‑1 (MQ=255) tCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAAc > 1:64076/1‑143 (MQ=255) gTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTCTGGTTCGGTGGCTCGTCTGGCGGCGGAAACAGGCATTCCGGTTTATGCGCCGGATAACGtt > 1:64037/1‑143 (MQ=255) aTTGAATCCCTCCGCGCATCCGGTGACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCTGTGAAAAAGCCTTTTAGGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTaa < 2:490336/143‑1 (MQ=255) aaGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTCTTATGGTTCGGTGGCTCGTCTGCCTGCGAAAAGAGGCATTCCAGTTTATGCGGCGGCACACGATAatcat > 2:44574/1‑143 (MQ=255) cccTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGACGTAAAGACGCATTCCGGGTTATGCGCCGGATAACGTTAATCATCCg > 1:148825/1‑143 (MQ=255) ccTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGTTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGc < 1:523291/143‑1 (MQ=255) ctcccggccgCCGATTACGAACTTAACGCCATTGTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCCGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCt < 2:611210/138‑1 (MQ=255) cctggccgCCGGTTACGAAATGCGCGCCATTTTGACCCATACTGATCATCCCGCCGAGAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCtgt < 2:458854/142‑1 (MQ=255) tggccgCCGGTTACGAAATTAGCGCCATTTGTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTgtgg > 1:232738/1‑143 (MQ=255) | TCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTGTGG > NC_000913/2367963‑2368239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |