| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 124,241 | C→T | 100% | Q409* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 124,241 | 0 | C | T | 95.8% | 64.8 / ‑6.3 | 29 | Q409* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (14/11); minor base G (2/2); total (16/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
GATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGC > NC_000913/124112‑124361 | aaTCCGAAGAAAATCTACGCGGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAAt < 1:505340/142‑1 (MQ=255) gaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACGAGGTTAAGAAAATGAACATgg > 1:238866/1‑143 (MQ=255) aaaaTCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGa > 2:439701/1‑143 (MQ=255) cTACGCTGCATTCAAGAAAGCGCAGGAAACCCAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGgtg < 1:194190/143‑1 (MQ=255) tGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTCGGTTAAGAAAATGAACATGGACGGTGTGCGTc > 2:134955/1‑143 (MQ=255) aTTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAACGGTTACGGCAGGGGCGACGCGGCTGAAGGTAACAACATAGCGCACGAGGGTAAGAGAATGGACATGGACGGTGGGCGGCatg > 2:656766/1‑142 (MQ=255) ttCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCAGAAGGTAAGAACATCGCGCACTAGGTCAAGAAAATGAACATGGACGGTGTGCGTCatat > 2:441753/1‑143 (MQ=255) ttCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCGCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAGAACATCGCGCACTAGGCCAAGACAATGAACATGGACGGTGTGCGTCatat > 2:442279/1‑143 (MQ=255) gAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTGAAGAAAATGAACATGGACGGCGAGCGTGATAGCGGCGACCGtgtgga > 2:595468/1‑138 (MQ=255) cAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATCTGc > 1:23246/1‑143 (MQ=255) aaaGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGcc > 1:532011/1‑143 (MQ=255) aaGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCg < 2:276722/143‑1 (MQ=255) agCAAAGCTACAGGAATCCCTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGgt < 1:70033/142‑1 (MQ=255) gCGACAGTAATCCTTGCTCATACCATTAAAGTTTACGGCATGGGCGACGCGGCTGAAGGTAAAACCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGa < 2:273710/143‑1 (MQ=255) aGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAg > 1:469575/1‑143 (MQ=255) aGTAAACCCTGCTCATACAATTAAAGCTTACGGCATTGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGCTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGAGACCGTTTCAATGTGCCGGTGTCTGATGCAg < 1:110441/143‑1 (MQ=255) ttGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCAATCGGTTAAGAAGATGAACATGGACGGTGTGCGTCATATCAGCGACCGTTCCAATGTGCCGGTGTCTGATGCAGCTATCGaa > 1:150642/1‑143 (MQ=255) gCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaaaa < 2:33198/143‑1 (MQ=255) aaTACCATCAGCGGTCACGCCAGCGGCGACGGGGCTGATGGTAAAAACGTCGCGCACGAGGTTAAGAAAATGAACATGTACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACt < 2:382557/142‑1 (MQ=255) cATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGt < 1:216044/143‑1 (MQ=255) ggaaaggggGACGCGGCAAAAGGTAAAAACCACGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTccc < 1:22071/138‑1 (MQ=255) tGGGCGACGCGGCTGAAGGTAAAATCATCGCGCACTAGGTGAAGAATATGAACATGGACGGTGTGCGTCATATCTGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGaa < 2:211150/143‑1 (MQ=255) tGGGCGACGCGACTGAAGGTAAAAACATCGCGTACTAGGTTAAGAAAATGAACATGGACGGTGTGAGTCGTCTCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGaa < 2:495427/143‑1 (MQ=255) gggCGACGCGGCTGAAGGTAAAAACATCGGGCACGAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATAGCCGCGACCGTTTCAATGTGCCGGTGTCTGAGGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAg < 1:574897/143‑1 (MQ=255) gCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGt > 1:437082/1‑143 (MQ=255) cGACGCGGCTGCAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGCCTGTTTCAATGTGCCGGTGTCTGATGCAGACATCGAAAAACTGCCGGACATCACCTTTCCgcgaggaa > 2:372178/1‑136 (MQ=255) gACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAAAATGCCAGAATCTGCCGTCCATCCCCTTCCCGGAAGGTTc > 1:445213/1‑143 (MQ=255) cgGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGaa > 1:174737/1‑143 (MQ=255) tGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCCCCTTCCCGGAAGGTTCTggcgggg > 1:91989/1‑137 (MQ=255) | GATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGC > NC_000913/124112‑124361 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |