Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,041,634	A→C	100%	T108P (ACC→CCC)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,634	0	A	C	100.0%	124.5 / NA	40	T108P (ACC→CCC)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/21);  total (19/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCT  >  NC_000913/3041501‑3041767                                                                                                                                      |                                                                                                                                      cGAGGCCAAAGGTAAAATGTGGAGCAATTGACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCgcgc                                                                                                                              <  2:511794/143‑1 (MQ=255) cGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCgcgc                                                                                                                              <  1:108534/143‑1 (MQ=255)         aaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAgacgac                                                                                                                      <  1:281835/143‑1 (MQ=255)         aaGGTAAAAGGTGGAGCAAGATACGTCTCTTCCGCGACGGCGATGGCTTTGAATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAgacgac                                                                                                                      <  1:38565/143‑1 (MQ=255)            gTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAg                                                                                                                   >  1:190068/1‑143 (MQ=255)               aaaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTAAACGGCGCAGCGTGCGCGAACCGCAGCTGACTGAACTGAAAAAATATGCGGGAGTCCCTAAAGAGCCCATCGCGACAGACGACGAGCgc                                                                                                                >  2:22762/1‑142 (MQ=255)                    tgGAGCAATTTACGTCTGGTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGtgct                                                                                                           <  2:282396/143‑1 (MQ=255)                         cAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                                                                      >  2:625803/1‑143 (MQ=255)                              tACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGtt                                                                                                 <  2:621551/143‑1 (MQ=255)                                  tCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg                                                                                             <  1:625803/143‑1 (MQ=255)                                  tCTGGTCCGCGACGGCGATGGCGTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg                                                                                             <  2:220979/143‑1 (MQ=255)                                        ccGCGTCGGCGAGGGCGGGGCATGGGTGGCTGGGCGGAGGGTGCGTGAGCCGCAGCGGACTGAACGTAAAAAATAGGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                                                                                       <  2:191863/143‑1 (MQ=255)                                        ccGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                                                                                       <  2:306494/143‑1 (MQ=255)                                                  gATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcgc                                                                             >  1:627128/1‑143 (MQ=255)                                                        tttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCt                                                                       >  2:219825/1‑143 (MQ=255)                                                         tcgcatgcatGGACCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTCTTCAGGCGCGCGCCGCGCTg                                                                      <  2:577059/141‑1 (MQ=255)                                                           gCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGCTATTCTCTAAAGTGCCCACCGTGCCAGACGACGAGCGTGTGCCGCTTGCTGCTGCCGGCTTTCAGGCCCGCGCCGCCCTGGc                                                                    >  2:616478/1‑143 (MQ=255)                                                              tGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaaa                                                                 <  1:539343/143‑1 (MQ=255)                                                                 aTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct                                                              >  1:408312/1‑143 (MQ=255)                                                                   tGAACGGCGCAGCGTGCGGGAACCGCAGCTGACTGAACTGCAAAAACATGCGTCATTCTCTATAGTGCTCTTCGCGCCAGACGACCAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct                                                            <  2:197974/143‑1 (MQ=255)                                                                      aCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCCAATCTCTTTa                                                         <  2:267795/143‑1 (MQ=255)                                                                      aCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCACGCAAGACGACGAGCGTGTGTAGACTGGCTTGGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa                                                         >  1:122727/1‑143 (MQ=255)                                                                      aCGGCCCAGCGTGCGTGAACCGCAGCTCACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa                                                         <  2:220717/143‑1 (MQ=255)                                                                       cGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCAGCGCTGTCACAGctctcaag                                                        >  2:501094/1‑139 (MQ=255)                                                                             aGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATAGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTGTTTAGCGAACt                                                  >  1:623401/1‑143 (MQ=255)                                                                              gcgtgcgtGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAACTCTTGATCGGACTg                                                 >  1:406512/1‑143 (MQ=255)                                                                                     tgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTGGCGAACTGCCTGCga                                          >  1:175464/1‑143 (MQ=255)                                                                                     tgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCga                                          >  1:635108/1‑143 (MQ=255)                                                                                       aaCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTACAGTGCCCATCGCGCCAGACGACGGGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa                                        <  2:54311/143‑1 (MQ=255)                                                                                          cgccgcTGACTGAACTGCAGATATATGCGGTATTCTCTAATGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaagaa                                     <  2:233437/143‑1 (MQ=255)                                                                                          cGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGCGCGTGTGCTGCTTGGTGTTGCCGGATTTCAGGCGCGCGCCGCGCTGGCAAGTATCTCTCGCGCACTCCCAGCgaaagaa                                     >  2:254770/1‑143 (MQ=255)                                                                                                  agtgaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGt                             <  2:384267/141‑1 (MQ=255)                                                                                                    tgaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACCGGTAg                           >  1:349487/1‑143 (MQ=255)                                                                                                    ggaactgaaAAAATATGCGGTATTCTCCAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAg                           <  1:136509/142‑1 (MQ=255)                                                                                                      aactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGACAAACAGGTAGTc                         >  1:188581/1‑143 (MQ=255)                                                                                                          gaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAGTCTCTTTGGCGAACTGCCTTCGAAAGAAAAACAGGCAGTCAaag                     >  2:60906/1‑143 (MQ=255)                                                                                                          gaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGCAAGAAAAACAGGTAGTCAcag                     >  1:224852/1‑143 (MQ=255)                                                                                                            taaaaTGGGCGGGATTCTCTAAAGTGCCCGGCGCGCCAGAGGACGAGCGTGTGCTGCTTGGTGTTGCCGGTGTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaa                   <  2:407286/142‑1 (MQ=255)                                                                                                                atatGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgcg               <  1:153866/143‑1 (MQ=255)                                                                                                                             tctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTGTCAGGCGCGCGCCGCGCTCGGAACTCTCTTTAGCGAACTGCCTTCGAAAGAAACACAGGTAATAAAGGGAAGCGCGATCACTATggt  >  2:44556/1‑141 (MQ=255)                                                                                                                                      |                                                                                                                                      CGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCT  >  NC_000913/3041501‑3041767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑