| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,473,614 | C→T | 100% | W156* (TGG→TGA) | rpsG ← | 30S ribosomal subunit protein S7 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,473,614 | 0 | C | T | 97.9% | 117.7 / ‑5.7 | 40 | W156* (TGG→TGA) | rpsG | 30S ribosomal subunit protein S7 |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (17/22); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGT > NC_000913/3473475‑3473752 | cGATGTCACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTCCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCaa < 1:176779/143‑1 (MQ=255) gTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATACTCTACGGTAGTGTGCGAAc > 1:71385/1‑143 (MQ=255) cGATGGGGGTTGTACGAGCCATTTGTTTCCGCGTTTATCTTTTAGGCGTGCAATTTAAGTAGCCCAAAGCGGGCTGCTGACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGcc < 1:405366/143‑1 (MQ=255) aTGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCtt < 1:578789/143‑1 (MQ=255) aCGCGCCAGTTTTCTGCTCTTGTATCTCTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAATGGATAATCAACGGTAGTGTGCTAACGCCTTGTTGGCTTCgg < 2:36053/143‑1 (MQ=255) aCGAGCCATTTGTTTCCTCGGTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCgg < 2:6634/143‑1 (MQ=255) cGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATATAAGTAGCCTAAAGCGGACTGCTTACTGGAAGCGCCCGCCTGGTGACTAAACCTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCCTGTTGGCTTCGGc > 2:429771/1‑143 (MQ=255) gAGCCATTTGTTTCCTCTTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGcc < 1:131672/143‑1 (MQ=255) ccATTTGTTTCCTCGTTTATCTTTTAGGCGGTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGGCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGCGTGCGAACGCCTTGTTGGCTTCGGCCATa < 1:292766/143‑1 (MQ=255) ccATTTGTTTCCTCGTTTAACTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACAGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGcacta > 1:138768/1‑139 (MQ=255) tttGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAGGCGGGCTGCTTACTGGAAGCGACCGCCTGATCACTAAAACTCCGAACGGATCATCCACGGCAGGGTGCGAACGCGCTGTGGGCTTCGGCCATACgg > 2:200321/1‑143 (MQ=255) ttGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCGCGCCTGGTGACGAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGt < 1:363388/143‑1 (MQ=255) tttCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGaa < 1:238413/143‑1 (MQ=255) ttCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCGGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAAc < 1:611419/143‑1 (MQ=255) ccTCGTTTATCTTTTAGGCGTTCAAGTTAAGGAGCCCAAAGCGGGCTGCTGACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGAATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGt < 2:367545/143‑1 (MQ=255) tCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGGACGTCt > 1:253089/1‑143 (MQ=255) tgttATCTTTTAGGCGTTCAATTTAAGTAGCCAAAAGCGGGATGAGTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTc < 1:347348/141‑1 (MQ=255) aTCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGGAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGt < 1:513092/143‑1 (MQ=255) aTCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGt > 1:156719/1‑143 (MQ=255) cTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTCCTGGACGCGCCCGCGTGGTGACTAAAAGTCCGAAGGGATCGCCAACGGGAGTGTGCGCACGCCTTGTTGGCTTCGGCCATACGGTGAGGGTCTTCGCGtct > 1:26608/1‑141 (MQ=255) cccAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGCAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGtgtcc > 2:438010/1‑139 (MQ=255) cAAATCGGGCTGCTTACTGGAAGCGCCCGCCTGGCGACTAAAACTCCGAAGGGAGAATCACCGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATTATGTTAACGTCTCCACGTCATCCAACTGCTGTTCCTTTATTCTCTg > 2:426367/1‑143 (MQ=255) cAAAGCGGGATGCTTACGGGAAGCGGACGATTGGTCCTTGTATCGCCGAAGGGATAATCAACGGTAGGGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTCTCTGAACTGCAGTGCCTTTGTTTTCTg > 2:470361/1‑143 (MQ=255) cGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgcagcc > 2:339478/1‑142 (MQ=255) gggCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCAt < 1:334607/143‑1 (MQ=255) gctgctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGGCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCa > 1:24607/1‑143 (MQ=255) ctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGaaa < 1:498111/143‑1 (MQ=255) ctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGaaa > 1:20771/1‑143 (MQ=255) aCTGGACGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAgtt < 1:27018/143‑1 (MQ=255) cTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAgttc < 1:644658/143‑1 (MQ=255) tGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTCACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAgttcg > 1:508279/1‑143 (MQ=255) gctcgccTGCTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCa < 2:98155/140‑1 (MQ=255) ggTGACTAAAACTCCGAAGGGATAATCGACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCaga < 1:251390/143‑1 (MQ=255) gTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCGTCGGAAAGTTCGTTCGCCAGGCGCagag > 1:279882/1‑143 (MQ=255) tAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGCACGTATTCACGTTTTTTATCTGCATTAGCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATg < 1:151901/143‑1 (MQ=255) aaCTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGAt < 2:226899/143‑1 (MQ=255) aaCTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCACCAGAAAGTTCGCTCTCCAGGCGCAGAGCCATGGAt > 2:416239/1‑143 (MQ=255) ttcGAGGGGATAGTCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTa < 2:348376/141‑1 (MQ=255) gAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCa > 2:280201/1‑143 (MQ=255) aTAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGACAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGt > 2:340740/1‑143 (MQ=255) | CGATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGT > NC_000913/3473475‑3473752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |