| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,532,534 | G→C | 15.6% | A217G (GCC→GGC) | yjhP ← | KpLE2 phage‑like element; putative methyltransferase YjhP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,532,534 | 0 | G | C | 15.6% | 52.7 / 4.3 | 24 | A217G (GCC→GGC) | yjhP | KpLE2 phage‑like element; putative methyltransferase YjhP |
| Reads supporting (aligned to +/- strand): ref base G (10/10); new base C (2/2); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 | |||||||||||
CGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAA > NC_000913/4532402‑4532675 | cGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTCGGTCACGTAGCGTTTCGGCGCGCTGTTTAGTTCGGCCCTGACTTCGGCGACGACGTc > 1:58791/1‑143 (MQ=255) tatTTCCAGCCAGAAATACCGGGAAGCTGGTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGCCGCCGAAGTCagg < 2:446554/143‑3 (MQ=255) tagttCCACCCAGAAATACCGGGAACCTGTTGTTGCCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATc < 2:450803/140‑1 (MQ=255) gatTTCGCGCCAGAGATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTGCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATc < 1:637699/142‑1 (MQ=255) tttCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCACAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAATTCGGCCCTGACTTCGGCGGCGAAGTCATCGt > 1:63599/1‑143 (MQ=255) tgtCCGGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGt < 2:283526/141‑1 (MQ=255) agcgaTACCGGGAACCCGTTGTTACCGGGCGATGAGTGCGAACGCGCGCCAGCCAAAGCGTTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCg < 2:204691/139‑1 (MQ=255) gaCCGGGAACTTGTTGTTACTGGGCGATGATTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTcc < 2:27574/142‑1 (MQ=255) aCCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTcca < 1:67375/143‑1 (MQ=255) ccGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTccag > 2:54710/1‑143 (MQ=255) cAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGGGGCTTCATACCGGTc > 2:21791/1‑143 (MQ=255) aaaGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCCTTTTGCGGCTTCATACCGGGCCCCGc > 2:152398/1‑143 (MQ=255) aaaGCATTCCCGCGCGTAGGTCACGTAGAGTTTCGGCGCTATTTTGAGTTCGGCCCTGACTGCGGCGGCGGAGTCATCGACTGGAGGCGCGTCCAGCCAACGGCGCAGGGTCAGCCATCTTGCGGCTTCATACCGGTCCCAGc > 2:499595/1‑143 (MQ=255) gtCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCt < 2:631229/142‑1 (MQ=255) gcccGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTg < 1:133916/142‑1 (MQ=255) ccGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTggc > 1:579381/1‑142 (MQ=255) ccGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGt > 1:460611/1‑143 (MQ=255) cGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTccac > 1:242248/1‑143 (MQ=255) gcgATGTTTAGTTCGGCCCTGAATACGGCGGCGAAGTAAGCGTCAGGAATCGCTTCCAGCCCGCGGCGCATGGTCAGCAATTTTGAGGCGGCATACGGGGCCCAGCCCTCCGGGTCTTCGGCTACCTTCCCCACCACGTCAtc > 2:76862/1‑142 (MQ=255) tGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCGTTGTCGGTAGCCTCATCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCg < 1:649621/143‑1 (MQ=255) gCCCTGACTTCGGATGCGATGTCTACGTCAGCATTCGCTTCCAGCCAGCGGCGCATGGTCATCCATTTTGCGGCTTCATACCGTTCCCAGCCTTCCTGGTCTGCCATTACCATTTCCACCACGTCATAGCCGAGATCGTCGaa < 2:103007/143‑1 (MQ=255) gatGACTTCGCCGCCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACg > 2:518852/3‑143 (MQ=255) gatGACTTCGCCGCCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGACCGCCGAACg > 1:534358/3‑143 (MQ=255) tCGCCGGCGAAGTCATCGTCTGGATTCGCCGCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGAc < 1:404705/143‑1 (MQ=255) ggcggcGAAGGCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATCCCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACaa < 2:239826/143‑1 (MQ=255) | CGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAA > NC_000913/4532402‑4532675 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |