| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 124,241 | C→T | Q409* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 124,241 | 0 | C | T | 98.0% | 142.3 / NA | 49 | Q409* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (18/30); total (19/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGC > NC_000913/124111‑124377 | cgctCCGAAGCAAATCGACGCTGCATTCAAGACAGCGCAGGAAACCAAGGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGaaaa < 1:340003/140‑1 (MQ=255) gATCCCAAGAAAATCCACGCGGCATTCAAGAAAGCGCAGGAGACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAAt < 2:451824/143‑1 (MQ=255) aTCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATg > 1:318915/1‑143 (MQ=255) ctgaagaaAATTTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCTAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGaa < 2:372284/141‑1 (MQ=255) cGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAAc > 1:72243/1‑143 (MQ=255) aagaaAATCTACGCTGCATGCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACAt < 1:158438/143‑1 (MQ=255) aacaaaaTCTCCGCTGCATGCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACAt < 2:430618/140‑1 (MQ=255) agaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAGATGAACATg > 1:237714/1‑143 (MQ=255) gaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATCAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATgg < 2:453879/143‑1 (MQ=255) aTCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACgg > 1:904888/1‑143 (MQ=255) cTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGgtg < 1:388049/143‑1 (MQ=255) tACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGgtgt < 1:789129/143‑1 (MQ=255) aCGCTGCATTCAAGAAAGCGCAGGCAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCGGCAGGGAACAACATCGCCCACTCGGCTAAGAAAACGACCATGGACGGcgtg > 2:180102/1‑143 (MQ=255) gCTGCATTCAATACAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCg < 2:638208/143‑1 (MQ=255) aaGAAAGCGCAGGAAACCAACGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcg < 1:249940/143‑1 (MQ=255) aGGAAACCAAAGGCAAAGCGACCGCAATCCTTGCTCATACCATTAAAGGTAACGGCATGGCCGACGCGGCTGACGGTACAAACATCGCGCACCAGGTTAAGGCGGTGAACAAAGACGGTCTGCGTCATATCCGCGGGCGTTTc > 2:206052/1‑143 (MQ=255) gAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCaa > 1:775378/1‑143 (MQ=255) gAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACAGTTTCaa > 1:776072/1‑143 (MQ=255) gAAACCAAAGGCAAAGCGACAGGAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCaa > 1:697698/1‑143 (MQ=255) acaCCAAAGGCAAAGCGACAGTAATCCTTGCTCAGACCAGGAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAAGGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAAt < 1:958509/141‑1 (MQ=255) aCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAAtgt < 1:785219/143‑1 (MQ=255) aaggcaaggcGACAGTAACCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCg < 1:354930/143‑1 (MQ=255) aaGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCg > 1:39958/1‑143 (MQ=255) aGGCAAAGCGACAGTAACCCTTGCTCATCCCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCgg < 1:711476/143‑1 (MQ=255) ggCAGAGCGACAGTAATCCGTGCGCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACTGCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGgt < 2:264076/143‑1 (MQ=255) cAAAGGCACAGTAATCCTTGCTCATACCATTAAAGGGTACGGCATGGGCGACGCGGCTGAAGGTAAAAACGTCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGAGCGTCATATCCGCGACCGTTTCAATGTGCCGgtgt < 2:876471/143‑1 (MQ=255) cAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGgtgt < 2:39958/143‑1 (MQ=255) cAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTCCAATGTGCCGgtgt > 1:328727/1‑143 (MQ=255) agggACAGTAATCCTTGCTCACACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTg < 1:588604/140‑1 (MQ=255) cGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAAGGTGCCGGTGTCTGAt > 1:744071/1‑143 (MQ=255) aCAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGc > 2:24487/1‑143 (MQ=255) cAGTAATACTGGCTCATACCATAAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCa < 1:73141/143‑1 (MQ=255) tGCTCATACCATTAACGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaaa < 2:560006/143‑1 (MQ=255) ggcgcATACCATTACAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATACCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaaa < 1:698035/139‑1 (MQ=255) cgCTCATACCATTACAGGTTCCAGCATGGGCGCCGCGGCTGCAGGTACAGACATCGTGCAATTACTTAGCTAATTGTACATGGACGGTGGGCGTCCTACCCGAGCCCATTCAAATGTGTCGGTGTCTGATGCAGATATCGaaa < 2:300388/142‑1 (MQ=255) tACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGc > 2:68084/1‑143 (MQ=255) ttAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATGCGCGACCGGTTCAATGTGCCGGTGTCTGATGCGGATACCGACAACCCGCCGGAc > 2:448137/1‑143 (MQ=255) acgggTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACAt < 2:582364/140‑1 (MQ=255) aaGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATc > 1:85076/1‑143 (MQ=255) gTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGGCTGGTGCAGATAGCGAAAAACTGCCGCACaccacc > 1:93932/1‑143 (MQ=255) cGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTcc < 1:24487/143‑1 (MQ=255) ggCATAGGCGACGAGACTGAAGGTAAAAGCATCGCGTACTAGGTGCACACAATAAACATGTGCGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAACAACTGCCGTACATCACCTTccc < 2:172033/143‑1 (MQ=255) tGGGCAACGCGGCTGAAGTTAAAAACAGCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGaa < 2:145567/143‑1 (MQ=255) gcgGCTGAAGGTAAGAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATGACCTTCCCGGGAGGGTCTGa > 1:917377/1‑143 (MQ=255) aaGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGAGTGCGTCATATCCGCGACCGTTTCAATGTTCCGGTGTCTGATGCGGATATCGAAAAACCGCCATCCAACACCTCCCGGGAAGGGTCTGAAGAGCac > 2:292926/1‑142 (MQ=255) tAAAAACATCGCGCACTAACTAAAGAAAATGAACATGGACGGTGTGCGTCAAATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAATAAATGTAGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCt < 2:712680/143‑1 (MQ=255) aaaaCATCGCGCACTCGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTAt < 2:318915/143‑1 (MQ=255) cATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGTATACCTATCTGc < 1:813759/143‑1 (MQ=255) gtgcACTAGGTTAAGAAAATGAGCATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGc < 2:728955/141‑1 (MQ=255) | CGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGC > NC_000913/124111‑124377 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |