Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	97.8%	117.6 / NA	46	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (22/23);  minor base G (0/1);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGC  >  NC_000913/696331‑696607                                                                                                                                            |                                                                                                                                          gTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGa                                                                                                                                        >  1:608708/1‑143 (MQ=255)    gCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGCATGCCGGAATTAGAATc                                                                                                                                     >  2:87547/1‑143 (MQ=255)    gCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCGCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATc                                                                                                                                     <  1:217307/143‑1 (MQ=255)        cctaCATTGAAGGCTAAGACGAGACAACATCGCATCAGACATTGAATGAACGCAGAAAAGCGAAAAGCTCGCCGAAGCGAGCGTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGt                                                                                                                                 <  2:922802/140‑1 (MQ=37)        ccGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGt                                                                                                                                 <  1:796379/143‑1 (MQ=255)         cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGAAATAGATACTCGGAATGCCGGAATTAGAATCCGGTg                                                                                                                                >  1:181326/1‑143 (MQ=255)          gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGc                                                                                                                               <  1:14505/143‑1 (MQ=255)             aTTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCGGGTGCCtt                                                                                                                            >  1:344201/1‑143 (MQ=255)                      tAAGACGCGCCAACATCGCACTCGAGATTGAATGAACACAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                   <  1:392202/143‑1 (MQ=25)                         gACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGa                                                                                                                >  2:220287/1‑143 (MQ=255)                            gtgtCTAGACCGCATCCGACATGGTATGGTCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGTGTATTCGAACTTCGGAATGCCGGAATTAGAATCCGTTGCCTTACCGCTTGGCGATAc                                                                                                             <  2:137425/143‑1 (MQ=21)                               tCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGCTAcccc                                                                                                          >  2:628430/1‑143 (MQ=255)                                     tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                                                                                    >  1:237172/1‑143 (MQ=255)                                     gcGCATTCGCCATTGAGGGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                                                                                    <  2:102997/142‑1 (MQ=255)                                      cGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCCACTggg                                                                                                   >  1:172623/1‑143 (MQ=255)                                                 agaaatgaaCGAAGTTAAACAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACaa                                                                                        >  2:217332/4‑143 (MQ=21)                                                   gaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAgg                                                                                      >  1:625011/1‑143 (MQ=255)                                                   gaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACACGCTGGACTTACAAgg                                                                                      >  2:493105/1‑143 (MQ=21)                                                     atgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTGTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACCGGGGGCACTTaggtggga                                                                                    >  1:330400/1‑136 (MQ=21)                                                      tgaaCGCAGAAACGCCAAAAGCTCGCCGTAGCGAGCTTTTTGAACGCGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTaa                                                                                   <  1:427323/143‑1 (MQ=21)                                                        accataGAAAAGCACAGAGCTCGCCGAAGCTCGCTTTTCTAATGTTGCTGGCGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGc                                                                                 <  2:650798/138‑1 (MQ=21)                                                        aaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCACTTGCAAgggcagc                                                                                 >  2:956567/1‑138 (MQ=21)                                                               aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGGACTTAGAAGGTACGAGCCTTGa                                                                          >  1:269763/1‑143 (MQ=21)                                                                aaaGCACTATGCTCGCCGCCGCGGTCGTTTTTTATGTGGCTGGGGTACGAGGTGTCGAACCTCGGAATGCCGGAATTAGAGTCCGGTGGCTCACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGaa                                                                         <  2:319735/143‑1 (MQ=18)                                                                    ccaaaaGCTTGCCGCCGCGACCGGTGCTAATGTGGCTGGGGTACGAGGATTCGAGCCTCGGAGTGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATaaa                                                                     <  1:499863/141‑1 (MQ=18)                                                                      aacaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCGGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAtt                                                                   <  1:808850/140‑1 (MQ=255)                                                                      aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAtt                                                                   <  1:805522/143‑1 (MQ=255)                                                                            tCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCATTACCTCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgg                                                             >  1:729666/1‑143 (MQ=255)                                                                                 gAAGCGAGCTTTTTTAATGTGGCTGGGGTAAGAGGATTCGAACAGCGGAATGCCGGGATTAGAATACGGTGCCTTACCGCATGGCGATACCCCGACTGGGTGCAGTTACAAGGGAAGAATATAGACTAAATTGTCTggagaac                                                        >  2:808300/1‑138 (MQ=255)                                                                                        gCTTTTGTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAtt                                                 <  1:199795/143‑1 (MQ=255)                                                                                         cTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTc                                                <  2:563160/143‑1 (MQ=255)                                                                                           tttttAATGTGGCTGGGGTACCAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGa                                              <  2:821026/143‑1 (MQ=255)                                                                                            ttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTCCGCGCCTTGAATAAATTTGCTGGGGTACGAGGATTCGaa                                             >  1:824860/1‑143 (MQ=21)                                                                                            ttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGCCTTGAATAAATTGGCTGGGGTACGAGGCTTCGaa                                             >  1:569692/1‑143 (MQ=255)                                                                                               taatccggCTGGGGTACGAGGATTCGAACCTCGTAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCt                                          <  1:434855/137‑1 (MQ=255)                                                                                                 aTGTGGTTGGGGTACGAGGGTTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCg                                        <  2:401878/143‑1 (MQ=255)                                                                                                        tGGGGTAGGAGGATTTTCACCTCGGAAGGGCCGAATTTGAACCCCGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGcc                                 <  2:278338/143‑1 (MQ=37)                                                                                                          gggTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCgg                               <  1:428138/143‑1 (MQ=255)                                                                                                            gTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGGTTCGAACCTCGGAATGCCGGaa                             >  1:735410/1‑143 (MQ=255)                                                                                                               cGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa                          <  1:220287/143‑1 (MQ=255)                                                                                                                   gATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGCCTTGAATAAATTGGCTGGCGTACGAGGCTTCGAACCTCGGCATGCCGGCATCAGaac                      >  1:473930/1‑142 (MQ=25)                                                                                                                     ttCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAATGTCAGCGCCTTGACTAAATTGTCGGGGGTACGTGGATTCGACCCTAGGCGGGCCGGTCTCAGCATcc                    >  2:113905/1‑143 (MQ=18)                                                                                                                      tCGAACCTCGGAATGCCGGAATTAGTATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCg                   <  1:76918/143‑1 (MQ=255)                                                                                                                          gccTCGGAAGGCTGGGATGAGAATCCGGCGCCTTAGCGCTTGGCGATACGCCGACTGGCTGCACTTACGAGGTAGGCGGCTTGATTAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc               <  2:320055/142‑1 (MQ=21)                                                                                                                                      cGGAATTAGAATCCGGTGCCTCACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTCCGCGTCTTGAATAAACTGGCTGGGGTCCGAGGATTCGAACCTCGCAATGCCGGAATCAGAATCCGGTGCCTTACTGCtttc   >  2:467796/1‑141 (MQ=25)                                                                                                                                       ggAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc  <  1:159778/143‑1 (MQ=255)                                                                                                                                            |                                                                                                                                          GTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGC  >  NC_000913/696331‑696607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑