| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 760,485 | G→A | G594S (GGC→AGC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 760,485 | 0 | G | A | 94.1% | 77.5 / NA | 35 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (19/13); total (21/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTACAC > NC_000913/760345‑760624 | aCAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGCTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGCGAGAAAATGTTCGACTGgcgc > 2:280772/1‑143 (MQ=255) gTTGAGAGGGGGCGCCGGCAGGGGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGc < 2:801591/143‑1 (MQ=255) ttGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGCGAAACTGTTCGACTGGAGCGGGGCg > 1:850158/1‑143 (MQ=255) agaTGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGCTCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaa > 1:544313/1‑143 (MQ=255) agaTGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaa > 2:955658/1‑143 (MQ=255) gcgcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAGGGAGTTGAAATGCAGTGTCGCGTTGCCAAGATTTATGGCGATGGCCAGGCGATGGCTGCCGGTGAGACACTGTTCGACTGGGGCGGTGCGGAGAGCCTCg > 2:213894/1‑143 (MQ=255) cgcCTGCATGAGCTGGCGAAACGCACCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGACACTGTTCGACTGGAGCGGTGCGGAAAACCTCGc < 2:216405/143‑1 (MQ=255) cccgCAGGACCTGTCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCtt < 1:290653/140‑1 (MQ=255) tGCAGGCGCTGGCGAGACGCATCAGGACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTAc < 1:552594/143‑1 (MQ=255) gCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATGTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGACACTGTGCGGCAGCCCTCGCTTagg > 2:744323/1‑141 (MQ=255) gCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGc > 1:292043/1‑143 (MQ=255) gCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGCTGCGGACAACCTCGCTTACGTCAGGCTGGt > 2:908743/1‑143 (MQ=255) cACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGCAAACCTCGCTTGCGCCACGCTGGTTGGTGAAGGCCTTc > 1:24279/1‑143 (MQ=255) cACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGACAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGCAGGCATTc > 1:165982/1‑143 (MQ=255) tGCCGGAAGCAGTGGAGATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGtt < 2:275250/143‑1 (MQ=255) gTTGAAATGCAGTCTCGCGTTGCCAAGAGTTATGGCGGTCGCCAGGCGATGGCTGCCGGTGAGAACCTGTTCGACTGGAGCGGTGCGGAGAACCTCGCTTACGCCACGCGGGTTGATGAAGGCATTCCGGTTCGCGCGTCgag > 2:587535/1‑141 (MQ=255) tggaaTGCAGTCTCGCGTTGCCAAGATTGATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTg < 2:629117/140‑1 (MQ=255) ggaaTGCAGCCGCGCGTTGCCAAGATGTATGGCGATCGCCGGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGa < 2:689495/141‑1 (MQ=255) gAAATGCAGTCTCGCGTTGCCAAGCTTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGCGCGGAAACCCTCAGGTACGCCACGAGGGTTGGTGAAGGCATTAAGGTTCGCCTGTCGGGGGa > 2:785718/1‑143 (MQ=255) tGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATTGCTGCCGGGGAGAAACTGTTCGACTCGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCATGTCGTGAGAAGAc > 2:71562/1‑143 (MQ=255) cgcgTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTCCCGGTGAGGCACTGTTCGACTGCAGCGGTGCGGAACACCTCGCTTACGCCACGCTGGTTGATGGAGGCAGTCCGGCTCCGCTGTCGCGTGCACCCTCCGGTcg > 2:814109/1‑143 (MQ=255) tGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTa < 1:666346/143‑1 (MQ=255) gagggtttAGGGCGCTCGCCAGGCGATGGCGGGCGCGGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCGACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACct < 2:379357/138‑1 (MQ=255) aTGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAc < 1:930619/143‑1 (MQ=255) aTGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAc < 2:415488/143‑1 (MQ=255) cAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTGACGCCACGCTGGCTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGt > 1:573264/1‑143 (MQ=255) aGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTg > 1:110498/1‑143 (MQ=255) gATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATcc > 1:825635/1‑143 (MQ=255) tGGCTGCCGGTGAGAAGCTGTTCGACTGGAGCGGTGCGGAAATCCTCGCTGACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCcac < 2:633365/143‑1 (MQ=255) tGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTACGCCTGACGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCCCGCGGTGATCCACAAcc > 2:373370/1‑143 (MQ=255) tGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTGCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTa > 1:345546/1‑143 (MQ=255) gagaAACTGGTCGACTGGAGCGGTGCGGAAAGCCTCGCTTACGCCACGCTGGTTGATGAGGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTaa < 2:178696/143‑1 (MQ=255) aCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCCGCCGGGTGAAGACTCCGGTCGCGGTACCTTCGTCCACCGGCACGCGCGGAGCCACAACCAGGCGAACgcct > 2:24444/1‑140 (MQ=255) gACTGGAGCGTTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGGAGGCGTTCCGGTTCGCCTGTCGGCTGAAGACTCCGGACGCGGTCGCGTCTTCCACCGCCACGCGGCGATCCCCACCCAGCCGAACCGTTCCAcgcc > 2:494212/1‑140 (MQ=255) gACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGCAGACTCCGGTCGCGGTGCCTTCTCCCACCGCCTAGCGGTGTTCCACGACCAGTCTAGCGGTTGCACTTa > 1:197792/1‑143 (MQ=255) tGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTacac < 2:528568/143‑1 (MQ=255) | ACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTACAC > NC_000913/760345‑760624 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |