Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	124,241	C→T	100%	Q409* (CAG→TAG)	aceE →	pyruvate dehydrogenase E1 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	124,241	0	C	T	100.0%	32.3 / NA	14	Q409* (CAG→TAG)	aceE	pyruvate dehydrogenase E1 component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/9);  total (5/9)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCT  >  NC_000913/124115‑124378                                                                                                                               |                                                                                                                                          ccGAAGAAAATCTACTCTGCATTCAAGAAATCGCAGGAAACCAAAGTCAAAGCTACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGCAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGaa                                                                                                                           <  1:234533/143‑1 (MQ=255)                            aaaGTGCAGGACACCAATGGCAAAGCGACAGTAATCCGTGCTCATACCTTTAAAGGTTACGGCATGGGCGACGCGGCTGACGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGa                                                                                                <  2:50770/143‑1 (MQ=255)                                   aGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTc                                                                                         >  1:216191/1‑143 (MQ=255)                                   aGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTc                                                                                         >  2:330221/1‑143 (MQ=255)                                          cAATGGCAATGCGACTGTAATCCTTGCTCATCCCATTAATGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAATCATCTCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTTATATCCGCGACCGTTTCAATGTGc                                                                                  <  2:294117/143‑1 (MQ=255)                                                 aaaGCGACAGTACTCCTTGCTCATACCATTAAGGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTc                                                                           <  1:321684/143‑1 (MQ=255)                                                                    cTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaaaaa                                                        <  1:279074/143‑1 (MQ=255)                                                                        tACCATGAAAGGGTACCGCGTGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGATCATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGc                                                    <  2:130475/143‑1 (MQ=255)                                                                          tcgttAAAGGTTACGGCATGGGCGAGTCGGCTGAAGGTAAAGACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTGAATGTGCCGGTGTCTGATGCAGATATCGAAAAGCTGCCg                                                  <  2:65147/140‑1 (MQ=255)                                                                                    ttACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGGACTAGGATAAGAAAATGAACATGGACGGTGGGCGTCATATCCGCGACCGTTTCAATGAGCCGGTGGCTGAGACGGGAAGCGAAAAACAGCCGGACAATACCt                                        >  2:212185/1‑143 (MQ=255)                                                                                               gCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGt                             >  2:260879/1‑143 (MQ=255)                                                                                                 gACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTc                           <  1:330221/143‑1 (MQ=255)                                                                                                       gCTGAACGTGAATATATCGCGTACTAGGTTACTAATATTCATATGCACGGTGTGCGTCTTATCCGCGACTTTTTCAATGTGCCGGTGTCTGATGCTGATATCGAAAATCTGCCGTACTTCTCCTTCCCGGAAGGTTCTGAaga                     <  2:222808/143‑1 (MQ=255)                                                                                                                          cgcACTAGGTTAAGAAAATGAACATGGATGGTGTGCGTCATATCCGCGACAGCTTCAATGTGCAGGTGTCTGATGCAGATATGGAAAAACTGCCGTACATCACCTTCACGGAAGGTTATGAAGAGAATATCTATATGCAGGcg  >  2:173722/1‑142 (MQ=255)                                                                                                                               |                                                                                                                                          CCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCT  >  NC_000913/124115‑124378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑