| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 760,485 | G→A | 100% | G594S (GGC→AGC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 760,485 | 0 | G | A | 99.2% | 39.5 / ‑7.1 | 18 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (8/9); minor base T (0/1); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTA > NC_000913/760350‑760621 | agaatgaTGTAGCGTGTGTAGGAGCAGGCGAAACGCAGCAGCACGGTGCCGGAAGCAGTTGAAATGCAGGCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGc < 1:269682/138‑1 (MQ=255) ttGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGCAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGACCCTGTTCGACTGGAGCGGTGCg > 1:35609/1‑143 (MQ=255) aGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAGATGCAGTCTCGCGTTGCCACGCTTTATGGCGACCGCCAGGCGACGGCTGCCGGCGTGAAACTGTTCGACAGGAGCGGTGCGGGACAccac > 2:178789/1‑141 (MQ=255) gcgcCTGCGGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCg < 2:348977/143‑1 (MQ=255) tGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTAc < 2:151562/143‑1 (MQ=255) cAGCACGGTGGCGGAAGCAGTTGAAATGCAGGCTCGCGGTGCCAAGATTTATGGCGAGCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCa < 2:194084/143‑1 (MQ=255) cACGGTGCCGGAAGCAGCTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGCAACTGTTCGACTGGAGCGGTGCGGAAGACCTCGCTTACGCCACGCTGGCTGGTGAGGGCAGTc > 2:225390/1‑143 (MQ=255) cGGAAGCAGGTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc < 2:345175/143‑1 (MQ=255) ggAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGAGCGGAAAACCTCGCTTACGCCACGCTGGTTGAGGAAGGCATTCCGGtgaccc > 2:39973/1‑138 (MQ=255) ggAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCAACAACAGAGAAACTGCTCCACTGGAGCGGAGCGGAAAACCTCGCTTACGCCCCGCTGGCAGATGAAGGCACACCGGtcggca > 2:238220/1‑138 (MQ=255) aaaTGCGGTCTCGCGTTGCCAAGATTTATGGCTATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGaa < 2:163752/143‑1 (MQ=255) gTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACGCCg > 2:351949/1‑143 (MQ=255) cgTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTcgcg > 1:171885/1‑143 (MQ=255) cAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGATCGGTGCGGAAAACCTCACTTACGCCACGCTGATTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACct > 1:220404/1‑143 (MQ=255) gCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCGCGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCAcgcg < 2:289870/143‑1 (MQ=255) gggCGGTGGCTGCCGGTGGGACGCGTTTCTGCGGGTGCTGTGCCGAAATCCGGGCTTGCGCCACGCTGGGTGATGGAGGCATTCCGGTTCGCCTGTCGGGTGAGGCCTCCAGGCGCGGTACCTTCTTCCACCGCCActttttc < 2:298386/142‑7 (MQ=255) tGGCTCCCGGGCAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCATGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCcac < 1:46918/143‑1 (MQ=255) gACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTa < 2:171885/143‑1 (MQ=255) | GTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTA > NC_000913/760350‑760621 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |