| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,750,629 | A→G | 16.9% | Y129H (TAT→CAT) | ydhX ← | putative 4Fe‑4S ferredoxin‑type protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,750,629 | 0 | A | G | 16.9% | 39.7 / 4.1 | 25 | Y129H (TAT→CAT) | ydhX | putative 4Fe‑4S ferredoxin‑type protein |
| Reads supporting (aligned to +/- strand): ref base A (10/10); new base G (2/2); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01 | |||||||||||
TTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATACTGCGT > NC_000913/1750495‑1750767 | ttCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGc < 2:79940/143‑1 (MQ=255) gggCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGACAGTCGAGACTCAGCGCAGAGATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTAGGGACAAGGGCCAAGGCAATAACTGACGCCgca > 2:291611/1‑141 (MQ=255) cggACGCAAATGGGCGGAAATCGTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTCCCACTTGAGTGACAGGGTTAAGGCAGCGAACCTGGTACGGACAAGCGCCAATCCAATAACTGCAGCCGATGCACTGCgg < 2:347733/141‑2 (MQ=255) gCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGGCAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGAttttt > 1:202417/1‑143 (MQ=255) gCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATGACTGCAGCCGATGCACTGCGATTTTTCTACCCGc > 2:212261/1‑143 (MQ=255) gAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTCTCTGCCACTTTAGTGACAGGGTTTAGGTCGCGGACCCGCCCCACAAATGGACTAAGTTTATAACTGCAGCCGATGCACTCCGACTTTTCTCCCCGCACg > 2:75187/1‑143 (MQ=255) gAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACg > 1:293013/1‑143 (MQ=255) cTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGCTTTTTCTACCCGCACGATcccc > 1:52477/1‑143 (MQ=255) cTTTTACGAGTCGGGACACAGCGAAGAAAGCACATGTAGCGGCCACTGTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATcccc < 1:133986/143‑1 (MQ=255) aGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATGACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGACCGACACCCCACGCCGccccc > 2:170942/1‑143 (MQ=255) cacaTTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGACCCCGTCggg > 1:288907/1‑143 (MQ=255) ttATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATGACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAAc < 1:170942/143‑1 (MQ=255) cTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATTCTTTATCTGCAGGCGACGCACTCCGACGTGTCTTCCCCCCCGATCCCCTGCCCATCCCGCCACGACGCCCCCGTCGGGCAAACGTCg < 2:164101/143‑1 (MQ=255) aCTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCa < 2:52477/143‑1 (MQ=255) aCTTTAGTGACAGAGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTCCTACCCGCACGATCCCCTGCTCATCACGCAACGACGCCCCCGGCGGGCAAAAGTCGATGcc > 2:25037/1‑142 (MQ=255) tgtcgtgtCAGGGTTATGGCATCGAACCTGGTACGGACAAGCGCCAATGCAATACCTGCCGCCGATGCACTGCGATTTTTCTACCCTCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACg < 2:187506/139‑1 (MQ=255) aGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATGACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAg < 1:212261/143‑1 (MQ=255) tGACAGGGTTAAGGTAGCGAACATGGTACGGACAAGCGCCAATGCAGTAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGACCCCCTGCTCATCACGCCACGACGCCCCCGCAGAGCACACGTCGATGCACgcagca > 2:56229/1‑143 (MQ=255) aGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGAACTACGATTTTTCTACCCGCACGATCCCCTGCTCATCATGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCtt < 2:125814/143‑1 (MQ=255) ttAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCa > 1:324981/1‑143 (MQ=255) ggTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGACGCACGGACCACCTTCGCCGTGc > 2:167812/1‑143 (MQ=255) gTAGCGAACCTGGTACGGACAAGAGCCAATCCAGTTACGGCATCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCt > 1:249370/1‑143 (MQ=255) aGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGt < 1:241082/143‑1 (MQ=255) ccAATGCAATAACTGCAGCCGTTGCACTACCAGTTTTGTGTCCCCACCACCCCCTGCTCATCACGTCACGACGCCCCCGGCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATa < 1:262793/143‑1 (MQ=255) ccaTAACTGCGCCCCATGCACCGCGATTTTTCTCGCCGGTTGTTCCCCTGCTCATCTCGCCACGACGCCCCCGCCTGGTAAACGTCGATGCACTGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATACTGCGt < 2:97849/141‑1 (MQ=255) | TTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATACTGCGT > NC_000913/1750495‑1750767 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |