| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,368,101 | T→C | 100% | A21A (GCT→GCC) | arnA → | fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,368,101 | 0 | T | C | 93.8% | 17.3 / ‑6.2 | 10 | A21A (GCT→GCC) | arnA | fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (4/5); minor base A (1/0); total (5/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCT > NC_000913/2367963‑2368235 | tCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGccgccg < 1:260024/143‑1 (MQ=255) acCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCCTCCCGCTTGGAAATTGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCAGCCGGTTAc > 1:76287/1‑143 (MQ=255) cgcgCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCATCGTTTTTACAAACAACAATAAAGAATGACTCGATATTGAAGCCCTGCTGGCCGCCGTTTACGAAATAAgc > 1:239606/1‑143 (MQ=255) aTTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTTTTTCTTTTTGGGATTCTTTGGTATTGAAGCCCTGATGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATa < 1:226403/143‑1 (MQ=255) ttttGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCCGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATAc > 2:360222/1‑143 (MQ=255) tGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGa < 2:222082/143‑1 (MQ=255) gTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCACTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGGGACAAAg > 2:176011/1‑143 (MQ=255) caGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTAACACGATATGGGATGCCTCGGTATTGAAGCGCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACACAGACTGGTAATAGCGGTGAAAAAggcagata > 2:153912/1‑136 (MQ=255) gCAAGGAAAATGAATAATGAAATCCGTCGTTTTTGCCTACCACGATGTGGGATGCCTGGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCTCGGTGAAAAAGCCTTTTATg < 2:308262/143‑1 (MQ=255) gggATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGTTATTCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATgcgc < 1:176011/143‑1 (MQ=255) ctgccgGCCGGCGGTTACGAAATGAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCt < 2:338575/143‑1 (MQ=255) | TCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCT > NC_000913/2367963‑2368235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |